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Article: Differential expression of sphingosine-1-phosphate receptors in abdominal aortic aneurysms

TitleDifferential expression of sphingosine-1-phosphate receptors in abdominal aortic aneurysms
Authors
Issue Date2012
Citation
Mediators Of Inflammation, 2012, v. 2012, article no. 643609 How to Cite?
AbstractObjective. Inflammation plays a key role in the pathophysiology of abdominal aortic aneurysms (AAAs). Newly discovered Sphingosine-1-Phosphate Receptors (S1P receptors) are critical in modulating inflammatory response via prostaglandin production. The aim of the current study was to investigate the expression of different S1P receptors in AAAs and compared with normal aortas at the protein level. Materials and Methods. Aortic specimens were harvested during aortic reconstructive surgery for the AAA group or during organ transplant for the control group. The protein expression of S1P1, 2 and 3 in AAAs and normal aortas was assessed by Western blotting and immunohistochemical analysis. Results. There were 40 AAAs and 20 control aortas collected for the receptor analysis. For Western blot analysis, S1P1 expression was not detected in either group; S1P2 protein was constitutively detected in both types of aortas but its expression level was significantly decreased by 73 (P < 0.05) in AAAs compared with the control group. In contrast, strong S1P3 expression was detected in AAAs aortas but not in normal aortas. Immumohistochemical staining showed similar results, except a weak S1P3 signal was detectable in normal aortas. Conclusions. Western blot and staining results consistently showed the down-regulation of the S1P2 protein with simultaneous up-regulation of the S1P3 protein in AAAs. Since those newly discovered receptors play an important role in the inflammatory cascade, the modulating of S1P signaling, particularly via S1P2 and S1P3, could represent novel therapeutic targets in future AAA treatments. Copyright 2012 Z. Qu et al.
Persistent Identifierhttp://hdl.handle.net/10722/169756
ISSN
2015 Impact Factor: 3.418
2015 SCImago Journal Rankings: 1.370
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorQu, Zen_US
dc.contributor.authorCheuk, BLYen_US
dc.contributor.authorCheng, SWKen_US
dc.date.accessioned2012-10-25T04:54:53Z-
dc.date.available2012-10-25T04:54:53Z-
dc.date.issued2012en_US
dc.identifier.citationMediators Of Inflammation, 2012, v. 2012, article no. 643609en_US
dc.identifier.issn0962-9351en_US
dc.identifier.urihttp://hdl.handle.net/10722/169756-
dc.description.abstractObjective. Inflammation plays a key role in the pathophysiology of abdominal aortic aneurysms (AAAs). Newly discovered Sphingosine-1-Phosphate Receptors (S1P receptors) are critical in modulating inflammatory response via prostaglandin production. The aim of the current study was to investigate the expression of different S1P receptors in AAAs and compared with normal aortas at the protein level. Materials and Methods. Aortic specimens were harvested during aortic reconstructive surgery for the AAA group or during organ transplant for the control group. The protein expression of S1P1, 2 and 3 in AAAs and normal aortas was assessed by Western blotting and immunohistochemical analysis. Results. There were 40 AAAs and 20 control aortas collected for the receptor analysis. For Western blot analysis, S1P1 expression was not detected in either group; S1P2 protein was constitutively detected in both types of aortas but its expression level was significantly decreased by 73 (P < 0.05) in AAAs compared with the control group. In contrast, strong S1P3 expression was detected in AAAs aortas but not in normal aortas. Immumohistochemical staining showed similar results, except a weak S1P3 signal was detectable in normal aortas. Conclusions. Western blot and staining results consistently showed the down-regulation of the S1P2 protein with simultaneous up-regulation of the S1P3 protein in AAAs. Since those newly discovered receptors play an important role in the inflammatory cascade, the modulating of S1P signaling, particularly via S1P2 and S1P3, could represent novel therapeutic targets in future AAA treatments. Copyright 2012 Z. Qu et al.en_US
dc.languageengen_US
dc.relation.ispartofMediators of Inflammationen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAorta - metabolism-
dc.subject.meshAortic Aneurysm, Abdominal - metabolism-
dc.subject.meshImmunohistochemistry - methods-
dc.subject.meshProstaglandins - metabolism-
dc.subject.meshReceptors, Lysosphingolipid - metabolism-
dc.titleDifferential expression of sphingosine-1-phosphate receptors in abdominal aortic aneurysmsen_US
dc.typeArticleen_US
dc.identifier.emailCheuk, BLY: bernice@hku.hken_US
dc.identifier.emailCheng, SWK: wkcheng@hkucc.hku.hken_US
dc.identifier.authorityCheuk, BLY=rp01671en_US
dc.identifier.authorityCheng, SWK=rp00374en_US
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1155/2012/643609en_US
dc.identifier.pmid22547907-
dc.identifier.pmcidPMC3323867-
dc.identifier.scopuseid_2-s2.0-84861063929en_US
dc.identifier.hkuros199407-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84861063929&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume2012en_US
dc.identifier.isiWOS:000302652300001-
dc.identifier.scopusauthoridQu, Z=55218389400en_US
dc.identifier.scopusauthoridCheuk, BLY=7801343617en_US
dc.identifier.scopusauthoridCheng, SWK=7404684779en_US
dc.customcontrol.immutablejt 130514-

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