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Article: Cyclooxygenase-2 regulates apoptosis in rat epididymis through prostaglandin D 2 1

TitleCyclooxygenase-2 regulates apoptosis in rat epididymis through prostaglandin D 2 1
Authors
Issue Date2002
PublisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/
Citation
Biology Of Reproduction, 2002, v. 66 n. 2, p. 374-380 How to Cite?
AbstractIn previous studies, cyclooxygenase (COX)-1 and COX-2 isozymes have been detected in the rat epididymis. COX-1 mediates electrolyte and fluid secretion induced by a number of peptide hormones, including bradykinin, angiotensin, and endothelin, via local formation of prostaglandin (PG) E 2; however, the physiological role of COX-2 remains largely unknown. Marked apoptotic cell death in the rat epididymis following androgen depletion has been reported. Because expression of both COX isozymes is dependent on androgen, we investigated whether these isozymes control apoptosis in the epididymis. Apoptosis was detected in rat epididymal epithelial cells by in situ staining using the TUNEL method and by the presence of internucleosomal DNA fragmentation using capillary electrophoresis with laser-induced fluorescence detection. Specific COX inhibitors were used to delineate the roles of the 2 isozymes. There was no significant apoptotic cell death in normal and specific COX-1 inhibitor (SC-560)-treated epididymal cells. However, application of a specific COX-2 inhibitor (NS-398) induced apoptosis in a dose- and time-dependent manner. A similar apoptotic effect of COX-2 inhibitor was seen in the in vivo study. The drastic DNA fragmentation induced by COX-2 inhibitor could be reversed completely by PGD 2 and partially by PGE 2. In addition, the protective effect of PGD 2 against COX-2 inhibition was significantly blocked by a PGDP-receptor antagonist, BWA868C. These results indicate that the COX-2 products PGD 2 and, to a lesser extent, PGE 2 control apoptosis in cultured rat epididymal cells in vitro.
Persistent Identifierhttp://hdl.handle.net/10722/169744
ISSN
2015 Impact Factor: 3.471
2015 SCImago Journal Rankings: 1.646
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCheuk, BLYen_US
dc.contributor.authorCheng Chew, SBen_US
dc.contributor.authorFiscus, RRen_US
dc.contributor.authorWong, PYDen_US
dc.date.accessioned2012-10-25T04:54:48Z-
dc.date.available2012-10-25T04:54:48Z-
dc.date.issued2002en_US
dc.identifier.citationBiology Of Reproduction, 2002, v. 66 n. 2, p. 374-380en_US
dc.identifier.issn0006-3363en_US
dc.identifier.urihttp://hdl.handle.net/10722/169744-
dc.description.abstractIn previous studies, cyclooxygenase (COX)-1 and COX-2 isozymes have been detected in the rat epididymis. COX-1 mediates electrolyte and fluid secretion induced by a number of peptide hormones, including bradykinin, angiotensin, and endothelin, via local formation of prostaglandin (PG) E 2; however, the physiological role of COX-2 remains largely unknown. Marked apoptotic cell death in the rat epididymis following androgen depletion has been reported. Because expression of both COX isozymes is dependent on androgen, we investigated whether these isozymes control apoptosis in the epididymis. Apoptosis was detected in rat epididymal epithelial cells by in situ staining using the TUNEL method and by the presence of internucleosomal DNA fragmentation using capillary electrophoresis with laser-induced fluorescence detection. Specific COX inhibitors were used to delineate the roles of the 2 isozymes. There was no significant apoptotic cell death in normal and specific COX-1 inhibitor (SC-560)-treated epididymal cells. However, application of a specific COX-2 inhibitor (NS-398) induced apoptosis in a dose- and time-dependent manner. A similar apoptotic effect of COX-2 inhibitor was seen in the in vivo study. The drastic DNA fragmentation induced by COX-2 inhibitor could be reversed completely by PGD 2 and partially by PGE 2. In addition, the protective effect of PGD 2 against COX-2 inhibition was significantly blocked by a PGDP-receptor antagonist, BWA868C. These results indicate that the COX-2 products PGD 2 and, to a lesser extent, PGE 2 control apoptosis in cultured rat epididymal cells in vitro.en_US
dc.languageengen_US
dc.publisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/en_US
dc.relation.ispartofBiology of Reproductionen_US
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosis - Physiologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCyclooxygenase 1en_US
dc.subject.meshCyclooxygenase 2en_US
dc.subject.meshCyclooxygenase 2 Inhibitorsen_US
dc.subject.meshCyclooxygenase Inhibitors - Pharmacologyen_US
dc.subject.meshDna Fragmentationen_US
dc.subject.meshElectrophoresis, Capillaryen_US
dc.subject.meshEpididymis - Cytologyen_US
dc.subject.meshFluorescenceen_US
dc.subject.meshIn Situ Nick-End Labelingen_US
dc.subject.meshIsoenzymes - Physiologyen_US
dc.subject.meshLasersen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Proteinsen_US
dc.subject.meshNitrobenzenesen_US
dc.subject.meshProstaglandin D2 - Physiologyen_US
dc.subject.meshProstaglandin-Endoperoxide Synthases - Physiologyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshSulfonamidesen_US
dc.titleCyclooxygenase-2 regulates apoptosis in rat epididymis through prostaglandin D 2 1en_US
dc.typeArticleen_US
dc.identifier.emailCheuk, BLY: bernice@hku.hken_US
dc.identifier.authorityCheuk, BLY=rp01671en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1095/biolreprod66.2.374-
dc.identifier.pmid11804951-
dc.identifier.scopuseid_2-s2.0-0036155206en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036155206&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume66en_US
dc.identifier.issue2en_US
dc.identifier.spage374en_US
dc.identifier.epage380en_US
dc.identifier.isiWOS:000173481900015-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridCheuk, BLY=7801343617en_US
dc.identifier.scopusauthoridCheng Chew, SB=6701358810en_US
dc.identifier.scopusauthoridFiscus, RR=7004669350en_US
dc.identifier.scopusauthoridWong, PYD=7403980262en_US

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