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Article: In vitro and in vivo characteristics of stem cells derived from the periodontal ligament of human deciduous and permanent teeth
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TitleIn vitro and in vivo characteristics of stem cells derived from the periodontal ligament of human deciduous and permanent teeth
 
AuthorsSong, JS1
Kim, SO1
Kim, SH1
Choi, HJ1
Son, HK1
Jung, HS1
Kim, CS1
Lee, JH1
 
Issue Date2012
 
PublisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=263
 
CitationTissue Engineering - Part A, 2012, v. 18 n. 19-20, p. 2040-2051 [How to Cite?]
DOI: http://dx.doi.org/10.1089/ten.tea.2011.0318
 
AbstractIn many studies, adult stem cells have been found in human periodontal ligament (PDL), but in most cases they were found in the permanent teeth. The aim of the present study was to characterize stem cells from the PDL of deciduous teeth (dPDLSCs) and compare them with those from the PDL of permanent teeth (pPDLSCs). Stem cell markers were examined by a flow cytometric analysis. The results of in vitro differentiation into adipogenic and osteogenic lineages were analyzed by histochemical staining and quantitative reverse transcription-polymerase chain reaction (RT-PCR). The results of in vivo transplantation were analyzed by histological staining, immunohistochemical staining, and quantitative RT-PCR. There were no significant differences in the proliferation rate, cell cycle distribution, expressions of stem cell markers such as Stro-1 and CD146, or in vitro differentiation. The pPDLSC transplants made more typical cementum/PDL-like tissues and expressed more cementum/PDL-related genes (CP23 and collagen XII) than did the dPDLSC transplants. Together, these results suggest that pPDLSCs are better candidates for use in reconstructing periodontium. © Copyright 2012, Mary Ann Liebert, Inc.
 
ISSN1937-3341
2013 SCImago Journal Rankings: 1.683
 
DOIhttp://dx.doi.org/10.1089/ten.tea.2011.0318
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorSong, JS
 
dc.contributor.authorKim, SO
 
dc.contributor.authorKim, SH
 
dc.contributor.authorChoi, HJ
 
dc.contributor.authorSon, HK
 
dc.contributor.authorJung, HS
 
dc.contributor.authorKim, CS
 
dc.contributor.authorLee, JH
 
dc.date.accessioned2012-10-25T04:53:18Z
 
dc.date.available2012-10-25T04:53:18Z
 
dc.date.issued2012
 
dc.description.abstractIn many studies, adult stem cells have been found in human periodontal ligament (PDL), but in most cases they were found in the permanent teeth. The aim of the present study was to characterize stem cells from the PDL of deciduous teeth (dPDLSCs) and compare them with those from the PDL of permanent teeth (pPDLSCs). Stem cell markers were examined by a flow cytometric analysis. The results of in vitro differentiation into adipogenic and osteogenic lineages were analyzed by histochemical staining and quantitative reverse transcription-polymerase chain reaction (RT-PCR). The results of in vivo transplantation were analyzed by histological staining, immunohistochemical staining, and quantitative RT-PCR. There were no significant differences in the proliferation rate, cell cycle distribution, expressions of stem cell markers such as Stro-1 and CD146, or in vitro differentiation. The pPDLSC transplants made more typical cementum/PDL-like tissues and expressed more cementum/PDL-related genes (CP23 and collagen XII) than did the dPDLSC transplants. Together, these results suggest that pPDLSCs are better candidates for use in reconstructing periodontium. © Copyright 2012, Mary Ann Liebert, Inc.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationTissue Engineering - Part A, 2012, v. 18 n. 19-20, p. 2040-2051 [How to Cite?]
DOI: http://dx.doi.org/10.1089/ten.tea.2011.0318
 
dc.identifier.doihttp://dx.doi.org/10.1089/ten.tea.2011.0318
 
dc.identifier.epage2051
 
dc.identifier.issn1937-3341
2013 SCImago Journal Rankings: 1.683
 
dc.identifier.issue19-20
 
dc.identifier.pmid22571499
 
dc.identifier.scopuseid_2-s2.0-84867009349
 
dc.identifier.spage2040
 
dc.identifier.urihttp://hdl.handle.net/10722/169596
 
dc.identifier.volume18
 
dc.languageeng
 
dc.publisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=263
 
dc.publisher.placeUnited States
 
dc.relation.ispartofTissue Engineering - Part A
 
dc.relation.referencesReferences in Scopus
 
dc.titleIn vitro and in vivo characteristics of stem cells derived from the periodontal ligament of human deciduous and permanent teeth
 
dc.typeArticle
 
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Author Affiliations
  1. Yonsei University