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Article: Rgs19 regulates mouse palatal fusion by modulating cell proliferation and apoptosis in the MEE
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TitleRgs19 regulates mouse palatal fusion by modulating cell proliferation and apoptosis in the MEE
 
AuthorsSohn, WJ2
Ji, YR2
Kim, HS2
Gwon, GJ2
Chae, YM2
An, CH2
Park, Hd1
Jung, HS3
Ryoo, ZY2
Lee, S2
Kim, JY2
 
KeywordsApoptosis
Genome Wide Screening
Palatal Fusion
Palatogenesis
Rgs19
 
Issue Date2012
 
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/modo
 
CitationMechanisms Of Development, 2012, v. 129 n. 9-12, p. 244-254 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.mod.2012.07.004
 
AbstractPalatal development is one of the critical events in craniofacial morphogenesis. During fusion of the palatal shelves, removal of the midline epithelial seam (MES) is a fundamental process for achieving proper morphogenesis of the palate. The reported mechanisms for removing the MES are the processes of apoptosis, migration or general epithelial-to-mesenchymal transition (EMT) through modulations of various signaling molecules including Wnt signaling. RGS19, a regulator of the G protein signaling (RGS) family, interacts selectively with the specific α subunits of the G proteins (Gαi, Gαq) and enhances their GTPase activity. Rgs19 was reported to be a modulator of the Wnt signaling pathway. In mouse palatogenesis, the restricted epithelial expression pattern of Rgs19 was examined in the palatal shelves, where expression of Wnt11 was observed. Based on these specific expression patterns of Rgs19 in the palatal shelves, the present study examined the detailed developmental function of Rgs19 using AS-ODN treatments during in vitro palate organ cultivations as a loss-of-function study. After the knockdown of Rgs19, the morphological changes in the palatal shelves was examined carefully using a computer-aided three dimensional reconstruction method and the altered expression patterns of related signaling molecules were evaluated using genome wide screening methods. RT-qPCR and in situ hybridization methods were also used to confirm these array results. These morphological and molecular examinations suggested that Rgs19 plays important roles in palatal fusion through the degradation of MES via activation of the palatal fusion related and apoptotic related genes. Overall, inhibition of the proliferation related and Wnt responsive genes by Rgs19 are required for proper palatal fusion. © 2012 Elsevier Ireland Ltd. All rights reserved.
 
ISSN0925-4773
2013 Impact Factor: 2.238
2013 SCImago Journal Rankings: 1.424
 
DOIhttp://dx.doi.org/10.1016/j.mod.2012.07.004
 
DC FieldValue
dc.contributor.authorSohn, WJ
 
dc.contributor.authorJi, YR
 
dc.contributor.authorKim, HS
 
dc.contributor.authorGwon, GJ
 
dc.contributor.authorChae, YM
 
dc.contributor.authorAn, CH
 
dc.contributor.authorPark, Hd
 
dc.contributor.authorJung, HS
 
dc.contributor.authorRyoo, ZY
 
dc.contributor.authorLee, S
 
dc.contributor.authorKim, JY
 
dc.date.accessioned2012-10-25T04:53:17Z
 
dc.date.available2012-10-25T04:53:17Z
 
dc.date.issued2012
 
dc.description.abstractPalatal development is one of the critical events in craniofacial morphogenesis. During fusion of the palatal shelves, removal of the midline epithelial seam (MES) is a fundamental process for achieving proper morphogenesis of the palate. The reported mechanisms for removing the MES are the processes of apoptosis, migration or general epithelial-to-mesenchymal transition (EMT) through modulations of various signaling molecules including Wnt signaling. RGS19, a regulator of the G protein signaling (RGS) family, interacts selectively with the specific α subunits of the G proteins (Gαi, Gαq) and enhances their GTPase activity. Rgs19 was reported to be a modulator of the Wnt signaling pathway. In mouse palatogenesis, the restricted epithelial expression pattern of Rgs19 was examined in the palatal shelves, where expression of Wnt11 was observed. Based on these specific expression patterns of Rgs19 in the palatal shelves, the present study examined the detailed developmental function of Rgs19 using AS-ODN treatments during in vitro palate organ cultivations as a loss-of-function study. After the knockdown of Rgs19, the morphological changes in the palatal shelves was examined carefully using a computer-aided three dimensional reconstruction method and the altered expression patterns of related signaling molecules were evaluated using genome wide screening methods. RT-qPCR and in situ hybridization methods were also used to confirm these array results. These morphological and molecular examinations suggested that Rgs19 plays important roles in palatal fusion through the degradation of MES via activation of the palatal fusion related and apoptotic related genes. Overall, inhibition of the proliferation related and Wnt responsive genes by Rgs19 are required for proper palatal fusion. © 2012 Elsevier Ireland Ltd. All rights reserved.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationMechanisms Of Development, 2012, v. 129 n. 9-12, p. 244-254 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.mod.2012.07.004
 
dc.identifier.citeulike11799673
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.mod.2012.07.004
 
dc.identifier.issn0925-4773
2013 Impact Factor: 2.238
2013 SCImago Journal Rankings: 1.424
 
dc.identifier.scopuseid_2-s2.0-84867899114
 
dc.identifier.urihttp://hdl.handle.net/10722/169594
 
dc.languageeng
 
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/modo
 
dc.publisher.placeIreland
 
dc.relation.ispartofMechanisms of Development
 
dc.subjectApoptosis
 
dc.subjectGenome Wide Screening
 
dc.subjectPalatal Fusion
 
dc.subjectPalatogenesis
 
dc.subjectRgs19
 
dc.titleRgs19 regulates mouse palatal fusion by modulating cell proliferation and apoptosis in the MEE
 
dc.typeArticle
 
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<contributor.author>Ji, YR</contributor.author>
<contributor.author>Kim, HS</contributor.author>
<contributor.author>Gwon, GJ</contributor.author>
<contributor.author>Chae, YM</contributor.author>
<contributor.author>An, CH</contributor.author>
<contributor.author>Park, Hd</contributor.author>
<contributor.author>Jung, HS</contributor.author>
<contributor.author>Ryoo, ZY</contributor.author>
<contributor.author>Lee, S</contributor.author>
<contributor.author>Kim, JY</contributor.author>
<date.accessioned>2012-10-25T04:53:17Z</date.accessioned>
<date.available>2012-10-25T04:53:17Z</date.available>
<date.issued>2012</date.issued>
<identifier.citation>Mechanisms Of Development, 2012, v. 129 n. 9-12, p. 244-254</identifier.citation>
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<description.abstract>Palatal development is one of the critical events in craniofacial morphogenesis. During fusion of the palatal shelves, removal of the midline epithelial seam (MES) is a fundamental process for achieving proper morphogenesis of the palate. The reported mechanisms for removing the MES are the processes of apoptosis, migration or general epithelial-to-mesenchymal transition (EMT) through modulations of various signaling molecules including Wnt signaling. RGS19, a regulator of the G protein signaling (RGS) family, interacts selectively with the specific &#945; subunits of the G proteins (G&#945;i, G&#945;q) and enhances their GTPase activity. Rgs19 was reported to be a modulator of the Wnt signaling pathway. In mouse palatogenesis, the restricted epithelial expression pattern of Rgs19 was examined in the palatal shelves, where expression of Wnt11 was observed. Based on these specific expression patterns of Rgs19 in the palatal shelves, the present study examined the detailed developmental function of Rgs19 using AS-ODN treatments during in vitro palate organ cultivations as a loss-of-function study. After the knockdown of Rgs19, the morphological changes in the palatal shelves was examined carefully using a computer-aided three dimensional reconstruction method and the altered expression patterns of related signaling molecules were evaluated using genome wide screening methods. RT-qPCR and in situ hybridization methods were also used to confirm these array results. These morphological and molecular examinations suggested that Rgs19 plays important roles in palatal fusion through the degradation of MES via activation of the palatal fusion related and apoptotic related genes. Overall, inhibition of the proliferation related and Wnt responsive genes by Rgs19 are required for proper palatal fusion. &#169; 2012 Elsevier Ireland Ltd. All rights reserved.</description.abstract>
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<subject>Apoptosis</subject>
<subject>Genome Wide Screening</subject>
<subject>Palatal Fusion</subject>
<subject>Palatogenesis</subject>
<subject>Rgs19</subject>
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Author Affiliations
  1. Forensic Science
  2. Kyungpook National University
  3. Yonsei Center of Biotechnology