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Article: Wnt5a plays a crucial role in determining tooth size during murine tooth development
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TitleWnt5a plays a crucial role in determining tooth size during murine tooth development
 
AuthorsCai, J3 4
Mutoh, N1
Shin, JO3
TaniIshii, N1
Ohshima, H2
Cho, SW3
Jung, HS3
 
Issue Date2011
 
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm
 
CitationCell And Tissue Research, 2011, v. 345 n. 3, p. 367-377 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00441-011-1224-4
 
AbstractWe have previously demonstrated that tooth size is determined by dental mesenchymal factors. Exogenous bone morphogenetic protein (BMP)4, Noggin, fibroblast growth factor (FGF)3 and FGF10 have no effect on tooth size, despite the expressions of Bmp2, Bmp4, Fgf3, Fgf10 and Lef1 in the dental mesenchyme. Among the wingless (Wnt) genes that are differentially expressed during tooth development, only Wnt5a is expressed in the dental mesenchyme. The aims of the present study were to clarify the expression pattern of Wnt5a in developing tooth germs and the role of Wnt5a in the regulation of tooth size by treatment with exogenous WNT5A with/without an apoptosis inhibitor on in vitro tooth germs combined with transplantation into kidney capsules. Wnt5a was intensely expressed in both the dental epithelium and mesenchyme during embryonic days 14-17, overlapping partly with the expressions of both Shh and Bmp4. Moreover, WNT5A retarded the development of tooth germs by markedly inducing cell death in the non-dental epithelium and mesenchyme but not widely in the dental region, where the epithelial-mesenchymal gene interactions among Wnt5a, Fgf10, Bmp4 and Shh might partly rescue the cells from death in the WNT5A-treated tooth germ. Together, these results indicate that WNT5A-induced cell death inhibited the overall development of the tooth germ, resulting in smaller teeth with blunter cusps after tooth-germ transplantation. Thus, it is suggested that Wnt5a is involved in regulating cell death in non-dental regions, while in the dental region it acts as a regulator of other genes that rescue tooth germs from cell death. © 2011 Springer-Verlag.
 
ISSN0302-766X
2013 Impact Factor: 3.333
 
DOIhttp://dx.doi.org/10.1007/s00441-011-1224-4
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorCai, J
 
dc.contributor.authorMutoh, N
 
dc.contributor.authorShin, JO
 
dc.contributor.authorTaniIshii, N
 
dc.contributor.authorOhshima, H
 
dc.contributor.authorCho, SW
 
dc.contributor.authorJung, HS
 
dc.date.accessioned2012-10-25T04:53:10Z
 
dc.date.available2012-10-25T04:53:10Z
 
dc.date.issued2011
 
dc.description.abstractWe have previously demonstrated that tooth size is determined by dental mesenchymal factors. Exogenous bone morphogenetic protein (BMP)4, Noggin, fibroblast growth factor (FGF)3 and FGF10 have no effect on tooth size, despite the expressions of Bmp2, Bmp4, Fgf3, Fgf10 and Lef1 in the dental mesenchyme. Among the wingless (Wnt) genes that are differentially expressed during tooth development, only Wnt5a is expressed in the dental mesenchyme. The aims of the present study were to clarify the expression pattern of Wnt5a in developing tooth germs and the role of Wnt5a in the regulation of tooth size by treatment with exogenous WNT5A with/without an apoptosis inhibitor on in vitro tooth germs combined with transplantation into kidney capsules. Wnt5a was intensely expressed in both the dental epithelium and mesenchyme during embryonic days 14-17, overlapping partly with the expressions of both Shh and Bmp4. Moreover, WNT5A retarded the development of tooth germs by markedly inducing cell death in the non-dental epithelium and mesenchyme but not widely in the dental region, where the epithelial-mesenchymal gene interactions among Wnt5a, Fgf10, Bmp4 and Shh might partly rescue the cells from death in the WNT5A-treated tooth germ. Together, these results indicate that WNT5A-induced cell death inhibited the overall development of the tooth germ, resulting in smaller teeth with blunter cusps after tooth-germ transplantation. Thus, it is suggested that Wnt5a is involved in regulating cell death in non-dental regions, while in the dental region it acts as a regulator of other genes that rescue tooth germs from cell death. © 2011 Springer-Verlag.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationCell And Tissue Research, 2011, v. 345 n. 3, p. 367-377 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00441-011-1224-4
 
dc.identifier.doihttp://dx.doi.org/10.1007/s00441-011-1224-4
 
dc.identifier.epage377
 
dc.identifier.issn0302-766X
2013 Impact Factor: 3.333
 
dc.identifier.issue3
 
dc.identifier.pmid21879290
 
dc.identifier.scopuseid_2-s2.0-80052775652
 
dc.identifier.spage367
 
dc.identifier.urihttp://hdl.handle.net/10722/169585
 
dc.identifier.volume345
 
dc.languageeng
 
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm
 
dc.publisher.placeGermany
 
dc.relation.ispartofCell and Tissue Research
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAmino Acid Chloromethyl Ketones - Pharmacology
 
dc.subject.meshAnimals
 
dc.subject.meshApoptosis - Drug Effects
 
dc.subject.meshBiological Assay
 
dc.subject.meshBody Patterning - Drug Effects
 
dc.subject.meshBone Morphogenetic Protein 4 - Genetics - Metabolism
 
dc.subject.meshBuffers
 
dc.subject.meshFibroblast Growth Factor 10 - Genetics - Metabolism
 
dc.subject.meshGene Expression Regulation, Developmental - Drug Effects
 
dc.subject.meshMice
 
dc.subject.meshMice, Inbred Icr
 
dc.subject.meshModels, Biological
 
dc.subject.meshOrgan Size - Drug Effects
 
dc.subject.meshTooth - Anatomy & Histology - Cytology - Drug Effects - Embryology
 
dc.subject.meshTooth Germ - Cytology - Drug Effects - Embryology - Metabolism
 
dc.subject.meshWnt Proteins - Genetics - Metabolism - Pharmacology
 
dc.titleWnt5a plays a crucial role in determining tooth size during murine tooth development
 
dc.typeArticle
 
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<contributor.author>Jung, HS</contributor.author>
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Author Affiliations
  1. Kanagawa Dental College
  2. Niigata University School of Medicine
  3. Yonsei University
  4. Chinese Academy of Sciences