Article: Wnt5a plays a crucial role in determining tooth size during murine tooth development

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TitleWnt5a plays a crucial role in determining tooth size during murine tooth development
AuthorsCai, J2 4
Mutoh, N1
Shin, JO2
TaniIshii, N1
Ohshima, H3
Cho, SW2
Jung, HS2
Issue Date2011
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm
CitationCell And Tissue Research, 2011, v. 345 n. 3, p. 367-377 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00441-011-1224-4
AbstractWe have previously demonstrated that tooth size is determined by dental mesenchymal factors. Exogenous bone morphogenetic protein (BMP)4, Noggin, fibroblast growth factor (FGF)3 and FGF10 have no effect on tooth size, despite the expressions of Bmp2, Bmp4, Fgf3, Fgf10 and Lef1 in the dental mesenchyme. Among the wingless (Wnt) genes that are differentially expressed during tooth development, only Wnt5a is expressed in the dental mesenchyme. The aims of the present study were to clarify the expression pattern of Wnt5a in developing tooth germs and the role of Wnt5a in the regulation of tooth size by treatment with exogenous WNT5A with/without an apoptosis inhibitor on in vitro tooth germs combined with transplantation into kidney capsules. Wnt5a was intensely expressed in both the dental epithelium and mesenchyme during embryonic days 14-17, overlapping partly with the expressions of both Shh and Bmp4. Moreover, WNT5A retarded the development of tooth germs by markedly inducing cell death in the non-dental epithelium and mesenchyme but not widely in the dental region, where the epithelial-mesenchymal gene interactions among Wnt5a, Fgf10, Bmp4 and Shh might partly rescue the cells from death in the WNT5A-treated tooth germ. Together, these results indicate that WNT5A-induced cell death inhibited the overall development of the tooth germ, resulting in smaller teeth with blunter cusps after tooth-germ transplantation. Thus, it is suggested that Wnt5a is involved in regulating cell death in non-dental regions, while in the dental region it acts as a regulator of other genes that rescue tooth germs from cell death. © 2011 Springer-Verlag.
ISSN0302-766X
2011 Impact Factor: 3.114
2011 SCImago Journal Rankings: 0.333
DOIhttp://dx.doi.org/10.1007/s00441-011-1224-4
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorCai, J
dc.contributor.authorMutoh, N
dc.contributor.authorShin, JO
dc.contributor.authorTaniIshii, N
dc.contributor.authorOhshima, H
dc.contributor.authorCho, SW
dc.contributor.authorJung, HS
dc.date.accessioned2012-10-25T04:53:10Z
dc.date.available2012-10-25T04:53:10Z
dc.date.issued2011
dc.description.abstractWe have previously demonstrated that tooth size is determined by dental mesenchymal factors. Exogenous bone morphogenetic protein (BMP)4, Noggin, fibroblast growth factor (FGF)3 and FGF10 have no effect on tooth size, despite the expressions of Bmp2, Bmp4, Fgf3, Fgf10 and Lef1 in the dental mesenchyme. Among the wingless (Wnt) genes that are differentially expressed during tooth development, only Wnt5a is expressed in the dental mesenchyme. The aims of the present study were to clarify the expression pattern of Wnt5a in developing tooth germs and the role of Wnt5a in the regulation of tooth size by treatment with exogenous WNT5A with/without an apoptosis inhibitor on in vitro tooth germs combined with transplantation into kidney capsules. Wnt5a was intensely expressed in both the dental epithelium and mesenchyme during embryonic days 14-17, overlapping partly with the expressions of both Shh and Bmp4. Moreover, WNT5A retarded the development of tooth germs by markedly inducing cell death in the non-dental epithelium and mesenchyme but not widely in the dental region, where the epithelial-mesenchymal gene interactions among Wnt5a, Fgf10, Bmp4 and Shh might partly rescue the cells from death in the WNT5A-treated tooth germ. Together, these results indicate that WNT5A-induced cell death inhibited the overall development of the tooth germ, resulting in smaller teeth with blunter cusps after tooth-germ transplantation. Thus, it is suggested that Wnt5a is involved in regulating cell death in non-dental regions, while in the dental region it acts as a regulator of other genes that rescue tooth germs from cell death. © 2011 Springer-Verlag.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationCell And Tissue Research, 2011, v. 345 n. 3, p. 367-377 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00441-011-1224-4
dc.identifier.doihttp://dx.doi.org/10.1007/s00441-011-1224-4
dc.identifier.epage377
dc.identifier.issn0302-766X
2011 Impact Factor: 3.114
2011 SCImago Journal Rankings: 0.333
dc.identifier.issue3
dc.identifier.pmid21879290
dc.identifier.scopuseid_2-s2.0-80052775652
dc.identifier.spage367
dc.identifier.urihttp://hdl.handle.net/10722/169585
dc.identifier.volume345
dc.languageeng
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm
dc.publisher.placeGermany
dc.relation.ispartofCell and Tissue Research
dc.relation.referencesReferences in Scopus
dc.subject.meshAmino Acid Chloromethyl Ketones - Pharmacology
dc.subject.meshAnimals
dc.subject.meshApoptosis - Drug Effects
dc.subject.meshBiological Assay
dc.subject.meshBody Patterning - Drug Effects
dc.subject.meshBone Morphogenetic Protein 4 - Genetics - Metabolism
dc.subject.meshBuffers
dc.subject.meshFibroblast Growth Factor 10 - Genetics - Metabolism
dc.subject.meshGene Expression Regulation, Developmental - Drug Effects
dc.subject.meshMice
dc.subject.meshMice, Inbred Icr
dc.subject.meshModels, Biological
dc.subject.meshOrgan Size - Drug Effects
dc.subject.meshTooth - Anatomy & Histology - Cytology - Drug Effects - Embryology
dc.subject.meshTooth Germ - Cytology - Drug Effects - Embryology - Metabolism
dc.subject.meshWnt Proteins - Genetics - Metabolism - Pharmacology
dc.titleWnt5a plays a crucial role in determining tooth size during murine tooth development
dc.typeArticle
Author Affiliations
  1. Kanagawa Dental College
  2. Yonsei University
  3. Niigata University School of Medicine
  4. Chinese Academy of Sciences