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Article: Wnt5a plays a crucial role in determining tooth size during murine tooth development

TitleWnt5a plays a crucial role in determining tooth size during murine tooth development
Authors
Issue Date2011
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm
Citation
Cell And Tissue Research, 2011, v. 345 n. 3, p. 367-377 How to Cite?
AbstractWe have previously demonstrated that tooth size is determined by dental mesenchymal factors. Exogenous bone morphogenetic protein (BMP)4, Noggin, fibroblast growth factor (FGF)3 and FGF10 have no effect on tooth size, despite the expressions of Bmp2, Bmp4, Fgf3, Fgf10 and Lef1 in the dental mesenchyme. Among the wingless (Wnt) genes that are differentially expressed during tooth development, only Wnt5a is expressed in the dental mesenchyme. The aims of the present study were to clarify the expression pattern of Wnt5a in developing tooth germs and the role of Wnt5a in the regulation of tooth size by treatment with exogenous WNT5A with/without an apoptosis inhibitor on in vitro tooth germs combined with transplantation into kidney capsules. Wnt5a was intensely expressed in both the dental epithelium and mesenchyme during embryonic days 14-17, overlapping partly with the expressions of both Shh and Bmp4. Moreover, WNT5A retarded the development of tooth germs by markedly inducing cell death in the non-dental epithelium and mesenchyme but not widely in the dental region, where the epithelial-mesenchymal gene interactions among Wnt5a, Fgf10, Bmp4 and Shh might partly rescue the cells from death in the WNT5A-treated tooth germ. Together, these results indicate that WNT5A-induced cell death inhibited the overall development of the tooth germ, resulting in smaller teeth with blunter cusps after tooth-germ transplantation. Thus, it is suggested that Wnt5a is involved in regulating cell death in non-dental regions, while in the dental region it acts as a regulator of other genes that rescue tooth germs from cell death. © 2011 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/169585
ISSN
2014 Impact Factor: 3.565
2014 SCImago Journal Rankings: 1.293
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCai, Jen_US
dc.contributor.authorMutoh, Nen_US
dc.contributor.authorShin, JOen_US
dc.contributor.authorTaniIshii, Nen_US
dc.contributor.authorOhshima, Hen_US
dc.contributor.authorCho, SWen_US
dc.contributor.authorJung, HSen_US
dc.date.accessioned2012-10-25T04:53:10Z-
dc.date.available2012-10-25T04:53:10Z-
dc.date.issued2011en_US
dc.identifier.citationCell And Tissue Research, 2011, v. 345 n. 3, p. 367-377en_US
dc.identifier.issn0302-766Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/169585-
dc.description.abstractWe have previously demonstrated that tooth size is determined by dental mesenchymal factors. Exogenous bone morphogenetic protein (BMP)4, Noggin, fibroblast growth factor (FGF)3 and FGF10 have no effect on tooth size, despite the expressions of Bmp2, Bmp4, Fgf3, Fgf10 and Lef1 in the dental mesenchyme. Among the wingless (Wnt) genes that are differentially expressed during tooth development, only Wnt5a is expressed in the dental mesenchyme. The aims of the present study were to clarify the expression pattern of Wnt5a in developing tooth germs and the role of Wnt5a in the regulation of tooth size by treatment with exogenous WNT5A with/without an apoptosis inhibitor on in vitro tooth germs combined with transplantation into kidney capsules. Wnt5a was intensely expressed in both the dental epithelium and mesenchyme during embryonic days 14-17, overlapping partly with the expressions of both Shh and Bmp4. Moreover, WNT5A retarded the development of tooth germs by markedly inducing cell death in the non-dental epithelium and mesenchyme but not widely in the dental region, where the epithelial-mesenchymal gene interactions among Wnt5a, Fgf10, Bmp4 and Shh might partly rescue the cells from death in the WNT5A-treated tooth germ. Together, these results indicate that WNT5A-induced cell death inhibited the overall development of the tooth germ, resulting in smaller teeth with blunter cusps after tooth-germ transplantation. Thus, it is suggested that Wnt5a is involved in regulating cell death in non-dental regions, while in the dental region it acts as a regulator of other genes that rescue tooth germs from cell death. © 2011 Springer-Verlag.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htmen_US
dc.relation.ispartofCell and Tissue Researchen_US
dc.subject.meshAmino Acid Chloromethyl Ketones - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosis - Drug Effectsen_US
dc.subject.meshBiological Assayen_US
dc.subject.meshBody Patterning - Drug Effectsen_US
dc.subject.meshBone Morphogenetic Protein 4 - Genetics - Metabolismen_US
dc.subject.meshBuffersen_US
dc.subject.meshFibroblast Growth Factor 10 - Genetics - Metabolismen_US
dc.subject.meshGene Expression Regulation, Developmental - Drug Effectsen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Icren_US
dc.subject.meshModels, Biologicalen_US
dc.subject.meshOrgan Size - Drug Effectsen_US
dc.subject.meshTooth - Anatomy & Histology - Cytology - Drug Effects - Embryologyen_US
dc.subject.meshTooth Germ - Cytology - Drug Effects - Embryology - Metabolismen_US
dc.subject.meshWnt Proteins - Genetics - Metabolism - Pharmacologyen_US
dc.titleWnt5a plays a crucial role in determining tooth size during murine tooth developmenten_US
dc.typeArticleen_US
dc.identifier.emailJung, HS: hsjung@yuhs.acen_US
dc.identifier.authorityJung, HS=rp01683en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00441-011-1224-4en_US
dc.identifier.pmid21879290en_US
dc.identifier.scopuseid_2-s2.0-80052775652en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80052775652&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume345en_US
dc.identifier.issue3en_US
dc.identifier.spage367en_US
dc.identifier.epage377en_US
dc.identifier.isiWOS:000294681100007-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridCai, J=9246458800en_US
dc.identifier.scopusauthoridMutoh, N=21733750900en_US
dc.identifier.scopusauthoridShin, JO=37361704500en_US
dc.identifier.scopusauthoridTaniIshii, N=6602564578en_US
dc.identifier.scopusauthoridOhshima, H=7202879991en_US
dc.identifier.scopusauthoridCho, SW=32967447200en_US
dc.identifier.scopusauthoridJung, HS=7403030195en_US

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