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Article: Identification and characterization of adenovirus early region 1B-associated protein 5 as a surface marker on undifferentiated human embryonic stem cells

TitleIdentification and characterization of adenovirus early region 1B-associated protein 5 as a surface marker on undifferentiated human embryonic stem cells
Authors
Issue Date2011
PublisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/jht
Citation
Stem Cells And Development, 2011, v. 20 n. 4, p. 609-620 How to Cite?
AbstractPluripotent human embryonic stem cells (hESCs) provide appropriate systems for developmental studies and prospective donor cell sources for regenerative medicine. Identification of surface markers specific to hESCs is a prerequisite for studying hESC biology and can be used to generate clinical-level donor cell preparations that are free from tumorigenic undifferentiated hESCs. We previously reported the generation of monoclonal antibodies that specifically recognize hESC surface antigens using a decoy immunization strategy. In this study, we show that monoclonal antibody 57-C11 recognizes a phosphorylated form of adenovirus early region 1B-associated protein 5 (E1B-AP5). E1B-AP5 is a nuclear RNA-binding protein, but we report that 57-C11-reactive E1B-AP5 is expressed on the surface of undifferentiated hESCs. In undifferentiated hESCs, 57-C11-reactive E1B-AP5 is localized to SSEA3-, SSEA4-, TRA-1-60-, TRA-1-81-, OCT4-, SOX2-, and NANOG-positive hESCs. In mixtures of undifferentiated hESCs and hESC-derived neurons, 57-C11 exclusively recognizes undifferentiated hESCs but not hESC-derived neuronal cells. Further, the expression of 57-C11-reactive E1B-AP5 decreases upon differentiation. Our results demonstrate that 57-C11-reactive E1B-AP5 is a novel surface molecule that is involved in the undifferentiated state of hESCs. As far as we know, this is the first report demonstrating that heterogeneous nuclear RNA-binding protein is expressed on the surface of undifferentiated hESCs. © Copyright 2011, Mary Ann Liebert, Inc. 2011.
Persistent Identifierhttp://hdl.handle.net/10722/169578
ISSN
2015 Impact Factor: 3.777
2015 SCImago Journal Rankings: 1.703
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChoi, HSen_US
dc.contributor.authorKim, WTen_US
dc.contributor.authorKim, Hen_US
dc.contributor.authorKim, JJen_US
dc.contributor.authorKo, JYen_US
dc.contributor.authorLee, SWen_US
dc.contributor.authorJang, YJen_US
dc.contributor.authorKim, SJen_US
dc.contributor.authorLee, MJen_US
dc.contributor.authorJung, HSen_US
dc.contributor.authorKzhyshkowska, Jen_US
dc.contributor.authorUm, SJen_US
dc.contributor.authorLee, MYen_US
dc.contributor.authorLee, SHen_US
dc.contributor.authorKim, CHen_US
dc.contributor.authorRyu, CJen_US
dc.date.accessioned2012-10-25T04:53:04Z-
dc.date.available2012-10-25T04:53:04Z-
dc.date.issued2011en_US
dc.identifier.citationStem Cells And Development, 2011, v. 20 n. 4, p. 609-620en_US
dc.identifier.issn1547-3287en_US
dc.identifier.urihttp://hdl.handle.net/10722/169578-
dc.description.abstractPluripotent human embryonic stem cells (hESCs) provide appropriate systems for developmental studies and prospective donor cell sources for regenerative medicine. Identification of surface markers specific to hESCs is a prerequisite for studying hESC biology and can be used to generate clinical-level donor cell preparations that are free from tumorigenic undifferentiated hESCs. We previously reported the generation of monoclonal antibodies that specifically recognize hESC surface antigens using a decoy immunization strategy. In this study, we show that monoclonal antibody 57-C11 recognizes a phosphorylated form of adenovirus early region 1B-associated protein 5 (E1B-AP5). E1B-AP5 is a nuclear RNA-binding protein, but we report that 57-C11-reactive E1B-AP5 is expressed on the surface of undifferentiated hESCs. In undifferentiated hESCs, 57-C11-reactive E1B-AP5 is localized to SSEA3-, SSEA4-, TRA-1-60-, TRA-1-81-, OCT4-, SOX2-, and NANOG-positive hESCs. In mixtures of undifferentiated hESCs and hESC-derived neurons, 57-C11 exclusively recognizes undifferentiated hESCs but not hESC-derived neuronal cells. Further, the expression of 57-C11-reactive E1B-AP5 decreases upon differentiation. Our results demonstrate that 57-C11-reactive E1B-AP5 is a novel surface molecule that is involved in the undifferentiated state of hESCs. As far as we know, this is the first report demonstrating that heterogeneous nuclear RNA-binding protein is expressed on the surface of undifferentiated hESCs. © Copyright 2011, Mary Ann Liebert, Inc. 2011.en_US
dc.languageengen_US
dc.publisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/jhten_US
dc.relation.ispartofStem Cells and Developmenten_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Differentiation - Metabolismen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshCoculture Techniquesen_US
dc.subject.meshDown-Regulationen_US
dc.subject.meshEmbryonic Stem Cells - Cytology - Metabolismen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshHeterogeneous-Nuclear Ribonucleoproteins - Genetics - Metabolismen_US
dc.subject.meshHomeodomain Proteins - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoprecipitationen_US
dc.subject.meshMembrane Proteins - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNeoplastic Stem Cells - Metabolismen_US
dc.subject.meshNuclear Proteins - Genetics - Metabolismen_US
dc.subject.meshPhosphorylationen_US
dc.subject.meshPluripotent Stem Cells - Cytology - Metabolismen_US
dc.subject.meshSoxb1 Transcription Factors - Metabolismen_US
dc.subject.meshTranscription Factors - Genetics - Metabolismen_US
dc.titleIdentification and characterization of adenovirus early region 1B-associated protein 5 as a surface marker on undifferentiated human embryonic stem cellsen_US
dc.typeArticleen_US
dc.identifier.emailJung, HS: hsjung@yuhs.acen_US
dc.identifier.authorityJung, HS=rp01683en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1089/scd.2010.0265en_US
dc.identifier.pmid21083500-
dc.identifier.scopuseid_2-s2.0-79953700592en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79953700592&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume20en_US
dc.identifier.issue4en_US
dc.identifier.spage609en_US
dc.identifier.epage620en_US
dc.identifier.isiWOS:000289053000006-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChoi, HS=9735793100en_US
dc.identifier.scopusauthoridKim, WT=34770435300en_US
dc.identifier.scopusauthoridKim, H=47461405700en_US
dc.identifier.scopusauthoridKim, JJ=24344310900en_US
dc.identifier.scopusauthoridKo, JY=7402678450en_US
dc.identifier.scopusauthoridLee, SW=36071696900en_US
dc.identifier.scopusauthoridJang, YJ=8359341300en_US
dc.identifier.scopusauthoridKim, SJ=7601599770en_US
dc.identifier.scopusauthoridLee, MJ=35264472100en_US
dc.identifier.scopusauthoridJung, HS=7403030195en_US
dc.identifier.scopusauthoridKzhyshkowska, J=6603091281en_US
dc.identifier.scopusauthoridUm, SJ=7005044024en_US
dc.identifier.scopusauthoridLee, MY=34667980000en_US
dc.identifier.scopusauthoridLee, SH=36068032600en_US
dc.identifier.scopusauthoridKim, CH=34667941600en_US
dc.identifier.scopusauthoridRyu, CJ=7101906825en_US

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