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Article: Immunohistochemical Localization of Cytokeratins in the Junctional Region of Ectoderm and Endoderm

TitleImmunohistochemical Localization of Cytokeratins in the Junctional Region of Ectoderm and Endoderm
Authors
Issue Date2010
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/113463905
Citation
Anatomical Record, 2010, v. 293 n. 11, p. 1864-1872 How to Cite?
AbstractAlthough tridermic species have two junctional regions of ectoderm and endoderm between their epidermis and digestive tract, we actually know little about these particular boundaries. Cytokeratins are the major intermediate filaments of epithelial cells and show a high degree of tissue specificity. Therefore, to characterize the epithelial cells in the junctional region of ectoderm and endoderm, we immunohistochemically examined the localization of cytokeratins 5, 7/17, 14, 18, Sox17, and alpha-fetoprotein (AFP) in the oropharyngeal and anorectal regions during the mouse gastrulation process. At embryonic day (E) 9.5, cytokeratins 5, 7/17, 14, and 18 were detected in all epithelial cells of the oropharyngeal region. At E12.5, cytokeratin 5-positive cells were not observed in the middle area of the oral cavity; however, the immunoreactivity was strong in the anterior and posterior areas. The immunoreaction of cytokeratins 18 was seen only in the middle and posterior areas of the oral mucosa. Cytokeratins 7/17 and 14 were localized in all areas of the oropharyngeal region. Sox17 and AFP, which are endodermal markers, were detected in the middle and posterior areas of the oral mucosa, but not in the anterior area. Moreover, this same localization pattern of cytokeratins also existed in the anorectal region of the E12.5 embryo, suggesting that the localization of cytokeratins and endodermal markers might give an implication for the boundary between ectoderm and endoderm. These results also suggest that these cytokeratins are useful molecules for monitoring the epithelial cell differentiation in the junctional region of the germ layers. Anat Rec, 2010. © 2010 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/169573
ISSN
2015 Impact Factor: 1.507
2015 SCImago Journal Rankings: 0.778
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHosoya, Aen_US
dc.contributor.authorKwak, Sen_US
dc.contributor.authorKim, EJen_US
dc.contributor.authorLunny, DPen_US
dc.contributor.authorBirgitte Lane, Een_US
dc.contributor.authorCho, SWen_US
dc.contributor.authorJung, HSen_US
dc.date.accessioned2012-10-25T04:53:01Z-
dc.date.available2012-10-25T04:53:01Z-
dc.date.issued2010en_US
dc.identifier.citationAnatomical Record, 2010, v. 293 n. 11, p. 1864-1872en_US
dc.identifier.issn1932-8486en_US
dc.identifier.urihttp://hdl.handle.net/10722/169573-
dc.description.abstractAlthough tridermic species have two junctional regions of ectoderm and endoderm between their epidermis and digestive tract, we actually know little about these particular boundaries. Cytokeratins are the major intermediate filaments of epithelial cells and show a high degree of tissue specificity. Therefore, to characterize the epithelial cells in the junctional region of ectoderm and endoderm, we immunohistochemically examined the localization of cytokeratins 5, 7/17, 14, 18, Sox17, and alpha-fetoprotein (AFP) in the oropharyngeal and anorectal regions during the mouse gastrulation process. At embryonic day (E) 9.5, cytokeratins 5, 7/17, 14, and 18 were detected in all epithelial cells of the oropharyngeal region. At E12.5, cytokeratin 5-positive cells were not observed in the middle area of the oral cavity; however, the immunoreactivity was strong in the anterior and posterior areas. The immunoreaction of cytokeratins 18 was seen only in the middle and posterior areas of the oral mucosa. Cytokeratins 7/17 and 14 were localized in all areas of the oropharyngeal region. Sox17 and AFP, which are endodermal markers, were detected in the middle and posterior areas of the oral mucosa, but not in the anterior area. Moreover, this same localization pattern of cytokeratins also existed in the anorectal region of the E12.5 embryo, suggesting that the localization of cytokeratins and endodermal markers might give an implication for the boundary between ectoderm and endoderm. These results also suggest that these cytokeratins are useful molecules for monitoring the epithelial cell differentiation in the junctional region of the germ layers. Anat Rec, 2010. © 2010 Wiley-Liss, Inc.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/113463905en_US
dc.relation.ispartofAnatomical Recorden_US
dc.subject.meshAnal Canal - Cytology - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiological Markersen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshEctoderm - Cytology - Metabolismen_US
dc.subject.meshEmbryo, Mammalian - Cytology - Metabolismen_US
dc.subject.meshEndoderm - Cytology - Metabolismen_US
dc.subject.meshEpithelial Cells - Cytology - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshGastrulationen_US
dc.subject.meshIntercellular Junctions - Metabolismen_US
dc.subject.meshKeratin-14 - Metabolismen_US
dc.subject.meshKeratin-18 - Metabolismen_US
dc.subject.meshKeratin-5 - Metabolismen_US
dc.subject.meshKeratin-7 - Metabolismen_US
dc.subject.meshKeratins - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshOropharynx - Cytology - Metabolismen_US
dc.subject.meshPregnancyen_US
dc.subject.meshRectum - Cytology - Metabolismen_US
dc.titleImmunohistochemical Localization of Cytokeratins in the Junctional Region of Ectoderm and Endodermen_US
dc.typeArticleen_US
dc.identifier.emailJung, HS: hsjung@yuhs.acen_US
dc.identifier.authorityJung, HS=rp01683en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/ar.21233en_US
dc.identifier.pmid20818615-
dc.identifier.scopuseid_2-s2.0-78049482943en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78049482943&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume293en_US
dc.identifier.issue11en_US
dc.identifier.spage1864en_US
dc.identifier.epage1872en_US
dc.identifier.isiWOS:000283958300007-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridHosoya, A=8651007100en_US
dc.identifier.scopusauthoridKwak, S=7202607781en_US
dc.identifier.scopusauthoridKim, EJ=36064163200en_US
dc.identifier.scopusauthoridLunny, DP=6603385849en_US
dc.identifier.scopusauthoridBirgitte Lane, E=24319822100en_US
dc.identifier.scopusauthoridCho, SW=32967447200en_US
dc.identifier.scopusauthoridJung, HS=7403030195en_US

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