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Article: Mapping of BrdU label-retaining dental pulp cells in growing teeth and their regenerative capacity after injuries

TitleMapping of BrdU label-retaining dental pulp cells in growing teeth and their regenerative capacity after injuries
Authors
Issue Date2010
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00418/index.htm
Citation
Histochemistry And Cell Biology, 2010, v. 134 n. 3, p. 227-241 How to Cite?
AbstractRecent studies have demonstrated that human dental pulp contains adult stem cells. A pulse of the thymidine analog BrdU given to young animals at the optimal time could clarify where slow-cycling long-term label-retaining cells (LRCs), putative adult stem cells, reside in the pulp tissue. This study focuses on the mapping of LRCs in growing teeth and their regenerative capacity after tooth injuries. Two to seven peritoneal injections of BrdU into pregnant Wistar rats revealed slow-cycling long-term dense LRCs in the mature tissues of born animals. Numerous dense LRCs were postnatally decreased in number and reached a plateau at 4 weeks after birth when they mainly resided in the center of the dental pulp, associating with blood vessels. Mature dental pulp cells were stained with Hoechst 33342 and sorted into (<0.76%) side population cells using FACS, which included dense LRCs. Some dense LRCs co-expressed mesenchymal stem cell markers such as STRO-1 or CD146. Tooth injuries caused degeneration of the odontoblast layer, and newly differentiated odontoblast-like cells contained LRCs. Thus, dense LRCs in mature pulp tissues were supposed to be dental pulp stem cells possessing regenerative capacity for forming newly differentiated odontoblast-like cells. The present study proposes the new hypothesis that both granular and dense LRCs are equipped in the dental pulp and that the dense LRCs with proliferative capacity play crucial roles in the pulpal healing process following exogenous stimuli in cooperation with the granular LRCs. © 2010 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/169572
ISSN
2015 Impact Factor: 2.78
2015 SCImago Journal Rankings: 1.287
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorIshikawa, Yen_US
dc.contributor.authorIdaYonemochi, Hen_US
dc.contributor.authorSuzuki, Hen_US
dc.contributor.authorNakakuraOhshima, Ken_US
dc.contributor.authorJung, HSen_US
dc.contributor.authorHonda, MJen_US
dc.contributor.authorIshii, Yen_US
dc.contributor.authorWatanabe, Nen_US
dc.contributor.authorOhshima, Hen_US
dc.date.accessioned2012-10-25T04:53:00Z-
dc.date.available2012-10-25T04:53:00Z-
dc.date.issued2010en_US
dc.identifier.citationHistochemistry And Cell Biology, 2010, v. 134 n. 3, p. 227-241en_US
dc.identifier.issn0948-6143en_US
dc.identifier.urihttp://hdl.handle.net/10722/169572-
dc.description.abstractRecent studies have demonstrated that human dental pulp contains adult stem cells. A pulse of the thymidine analog BrdU given to young animals at the optimal time could clarify where slow-cycling long-term label-retaining cells (LRCs), putative adult stem cells, reside in the pulp tissue. This study focuses on the mapping of LRCs in growing teeth and their regenerative capacity after tooth injuries. Two to seven peritoneal injections of BrdU into pregnant Wistar rats revealed slow-cycling long-term dense LRCs in the mature tissues of born animals. Numerous dense LRCs were postnatally decreased in number and reached a plateau at 4 weeks after birth when they mainly resided in the center of the dental pulp, associating with blood vessels. Mature dental pulp cells were stained with Hoechst 33342 and sorted into (<0.76%) side population cells using FACS, which included dense LRCs. Some dense LRCs co-expressed mesenchymal stem cell markers such as STRO-1 or CD146. Tooth injuries caused degeneration of the odontoblast layer, and newly differentiated odontoblast-like cells contained LRCs. Thus, dense LRCs in mature pulp tissues were supposed to be dental pulp stem cells possessing regenerative capacity for forming newly differentiated odontoblast-like cells. The present study proposes the new hypothesis that both granular and dense LRCs are equipped in the dental pulp and that the dense LRCs with proliferative capacity play crucial roles in the pulpal healing process following exogenous stimuli in cooperation with the granular LRCs. © 2010 Springer-Verlag.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00418/index.htmen_US
dc.relation.ispartofHistochemistry and Cell Biologyen_US
dc.subject.meshAdult Stem Cells - Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBromodeoxyuridine - Diagnostic Useen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Proliferationen_US
dc.subject.meshDental Cavity Preparationen_US
dc.subject.meshDental Pulp - Cytology - Physiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshMesenchymal Stem Cells - Cytologyen_US
dc.subject.meshPregnancyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Wistaren_US
dc.subject.meshRegenerationen_US
dc.subject.meshSide-Population Cells - Cytologyen_US
dc.subject.meshTooth Injuries - Physiopathologyen_US
dc.subject.meshTooth Replantationen_US
dc.titleMapping of BrdU label-retaining dental pulp cells in growing teeth and their regenerative capacity after injuriesen_US
dc.typeArticleen_US
dc.identifier.emailJung, HS: hsjung@yuhs.acen_US
dc.identifier.authorityJung, HS=rp01683en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00418-010-0727-5en_US
dc.identifier.pmid20676671-
dc.identifier.scopuseid_2-s2.0-77956920668en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77956920668&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume134en_US
dc.identifier.issue3en_US
dc.identifier.spage227en_US
dc.identifier.epage241en_US
dc.identifier.isiWOS:000281249500001-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridIshikawa, Y=37026187600en_US
dc.identifier.scopusauthoridIdaYonemochi, H=6602645881en_US
dc.identifier.scopusauthoridSuzuki, H=8740460700en_US
dc.identifier.scopusauthoridNakakuraOhshima, K=6602464566en_US
dc.identifier.scopusauthoridJung, HS=7403030195en_US
dc.identifier.scopusauthoridHonda, MJ=35263957300en_US
dc.identifier.scopusauthoridIshii, Y=35366660600en_US
dc.identifier.scopusauthoridWatanabe, N=7403340913en_US
dc.identifier.scopusauthoridOhshima, H=7202879991en_US

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