File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Immunolocalization of laminin and integrin in regenerating junctional epithelium of mice after gingivectomy

TitleImmunolocalization of laminin and integrin in regenerating junctional epithelium of mice after gingivectomy
Authors
Issue Date2009
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1
Citation
Journal Of Periodontal Research, 2009, v. 44 n. 4, p. 489-495 How to Cite?
AbstractMasaoka T, Hashimoto S, Kinumatsu T, Muramatsu T, Jung H-S, Yamada S, Shimono M. Immunolocalization of laminin and integrin in regenerating junctional epithelium of mice after gingivectomy. J Periodont Res 2009; 44: 489-495. © 2008 The Authors. Journal compilation© 2008 Blackwell MunksgaardBackground and Objective: The expression patterns of adhesive proteins and extracellular matrix proteins in regenerating gingival epithelium after gingivectomy are unknown. The aim of this study was to examine the expression of laminin 1, laminin γ2 (a specific component of laminin 5), integrin β4 and integrin α3 in the regenerating gingival epithelium in order to understand the mechanism of wound healing during reconstitution of the sulcular environment. Material and Methods: The palatal gingivae of the maxillary molars of Institute of Cancer Research mice were excised, and the regenerating tissues were examined 1, 3, 5, 7 and 14 days later. Fresh, non-fixed and non-decalcified frozen sections were prepared and stained using immunofluorescence. Results: At 1 day post-surgery, intense expression of laminin γ2, integrin β4 and integrin α3 was distinct in the frontal margin of the regenerating oral epithelium. Laminin γ2 was diffusely detected on the root surface and in connective tissues beneath the regenerating oral epithelium at 3 and 5 days. At 7 days, laminin γ2 was intermittently recognizable in the internal basal lamina (IBL) close to tooth-facing cells, while laminin γ2, integrin β4 and integrin α3 were observed in the IBL and in the external basal lamina (EBL) of the regenerating junctional epithelium at 14 days. Conclusion: These results suggest that secretion of laminin 5 in the connective tissue may induce epithelial cell migration, and that binding of laminin 5 to integrin α6β4 and integrin α3β1 in the IBL may provoke cell adhesion and migration of cells facing the tooth on the enamel surface of the regenerating junctional epithelium. © 2008 Blackwell Munksgaard.
Persistent Identifierhttp://hdl.handle.net/10722/169562
ISSN
2015 Impact Factor: 2.474
2015 SCImago Journal Rankings: 0.932
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMasaoka, Ten_US
dc.contributor.authorHashimoto, Sen_US
dc.contributor.authorKinumatsu, Ten_US
dc.contributor.authorMuramatsu, Ten_US
dc.contributor.authorJung, HSen_US
dc.contributor.authorYamada, Sen_US
dc.contributor.authorShimono, Men_US
dc.date.accessioned2012-10-25T04:52:54Z-
dc.date.available2012-10-25T04:52:54Z-
dc.date.issued2009en_US
dc.identifier.citationJournal Of Periodontal Research, 2009, v. 44 n. 4, p. 489-495en_US
dc.identifier.issn0022-3484en_US
dc.identifier.urihttp://hdl.handle.net/10722/169562-
dc.description.abstractMasaoka T, Hashimoto S, Kinumatsu T, Muramatsu T, Jung H-S, Yamada S, Shimono M. Immunolocalization of laminin and integrin in regenerating junctional epithelium of mice after gingivectomy. J Periodont Res 2009; 44: 489-495. © 2008 The Authors. Journal compilation© 2008 Blackwell MunksgaardBackground and Objective: The expression patterns of adhesive proteins and extracellular matrix proteins in regenerating gingival epithelium after gingivectomy are unknown. The aim of this study was to examine the expression of laminin 1, laminin γ2 (a specific component of laminin 5), integrin β4 and integrin α3 in the regenerating gingival epithelium in order to understand the mechanism of wound healing during reconstitution of the sulcular environment. Material and Methods: The palatal gingivae of the maxillary molars of Institute of Cancer Research mice were excised, and the regenerating tissues were examined 1, 3, 5, 7 and 14 days later. Fresh, non-fixed and non-decalcified frozen sections were prepared and stained using immunofluorescence. Results: At 1 day post-surgery, intense expression of laminin γ2, integrin β4 and integrin α3 was distinct in the frontal margin of the regenerating oral epithelium. Laminin γ2 was diffusely detected on the root surface and in connective tissues beneath the regenerating oral epithelium at 3 and 5 days. At 7 days, laminin γ2 was intermittently recognizable in the internal basal lamina (IBL) close to tooth-facing cells, while laminin γ2, integrin β4 and integrin α3 were observed in the IBL and in the external basal lamina (EBL) of the regenerating junctional epithelium at 14 days. Conclusion: These results suggest that secretion of laminin 5 in the connective tissue may induce epithelial cell migration, and that binding of laminin 5 to integrin α6β4 and integrin α3β1 in the IBL may provoke cell adhesion and migration of cells facing the tooth on the enamel surface of the regenerating junctional epithelium. © 2008 Blackwell Munksgaard.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1en_US
dc.relation.ispartofJournal of Periodontal Researchen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBasement Membrane - Pathologyen_US
dc.subject.meshCell Adhesion - Physiologyen_US
dc.subject.meshCell Adhesion Molecules - Analysisen_US
dc.subject.meshCell Movement - Physiologyen_US
dc.subject.meshConnective Tissue - Pathologyen_US
dc.subject.meshEpithelial Attachment - Pathologyen_US
dc.subject.meshEpithelium - Pathologyen_US
dc.subject.meshGingiva - Pathologyen_US
dc.subject.meshGingivectomyen_US
dc.subject.meshIntegrin Alpha3 - Analysisen_US
dc.subject.meshIntegrin Beta4 - Analysisen_US
dc.subject.meshIntegrins - Analysisen_US
dc.subject.meshLaminin - Analysisen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Icren_US
dc.subject.meshRegeneration - Physiologyen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTooth Cervix - Pathologyen_US
dc.subject.meshTooth Root - Pathologyen_US
dc.titleImmunolocalization of laminin and integrin in regenerating junctional epithelium of mice after gingivectomyen_US
dc.typeArticleen_US
dc.identifier.emailJung, HS: hsjung@yuhs.acen_US
dc.identifier.authorityJung, HS=rp01683en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1600-0765.2008.01142.xen_US
dc.identifier.pmid18973515en_US
dc.identifier.scopuseid_2-s2.0-67650787419en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67650787419&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume44en_US
dc.identifier.issue4en_US
dc.identifier.spage489en_US
dc.identifier.epage495en_US
dc.identifier.isiWOS:000267426900009-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridMasaoka, T=35170444200en_US
dc.identifier.scopusauthoridHashimoto, S=35556628400en_US
dc.identifier.scopusauthoridKinumatsu, T=6507201640en_US
dc.identifier.scopusauthoridMuramatsu, T=7402581093en_US
dc.identifier.scopusauthoridJung, HS=7403030195en_US
dc.identifier.scopusauthoridYamada, S=7404921517en_US
dc.identifier.scopusauthoridShimono, M=7006406232en_US
dc.identifier.citeulike5007061-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats