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Article: Characteristics of ethylnitrosourea-induced cataracts
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TitleCharacteristics of ethylnitrosourea-induced cataracts
 
AuthorsLee, MJ1
Kim, JY2
Kim, YJ1
Cho, JW3
Cho, KH3
Song, CW3
Jung, HS1
 
Issue Date2009
 
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/02713683.asp
 
CitationCurrent Eye Research, 2009, v. 34 n. 5, p. 360-368 [How to Cite?]
DOI: http://dx.doi.org/10.1080/02713680902843047
 
AbstractPurpose: This study analyzed genes associated with the morphology and regulation of ethylnitrosourea (ENU)-induced cataract mouse. Materials and Methods: Immunohistochemistry analysis using anti-crystallins and PCNA antibody revealed that the localization pattern of these specific markers differed between the cataractous and wild-type lens epithelium. Two-dimensional electrophoresis and microarray techniques were used to identify the proteins and genes related to ENU-induced cataract. Results: A novel ENU-induced mutation in the mouse led to nuclear and cortical opacity of the eye lens at 5 weeks postnatal. This cataract phenotype was similar to that of the zonular-pulverulent type of human cataract. Crystallin proteins and gap-junction genes have relations to the formation of cataract. Conclusions: Together, the results suggest that various proteins affect the formation and specific phenotypes of ENU-induced cataract mouse.
 
ISSN0271-3683
2013 Impact Factor: 1.663
2013 SCImago Journal Rankings: 0.977
 
DOIhttp://dx.doi.org/10.1080/02713680902843047
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLee, MJ
 
dc.contributor.authorKim, JY
 
dc.contributor.authorKim, YJ
 
dc.contributor.authorCho, JW
 
dc.contributor.authorCho, KH
 
dc.contributor.authorSong, CW
 
dc.contributor.authorJung, HS
 
dc.date.accessioned2012-10-25T04:52:52Z
 
dc.date.available2012-10-25T04:52:52Z
 
dc.date.issued2009
 
dc.description.abstractPurpose: This study analyzed genes associated with the morphology and regulation of ethylnitrosourea (ENU)-induced cataract mouse. Materials and Methods: Immunohistochemistry analysis using anti-crystallins and PCNA antibody revealed that the localization pattern of these specific markers differed between the cataractous and wild-type lens epithelium. Two-dimensional electrophoresis and microarray techniques were used to identify the proteins and genes related to ENU-induced cataract. Results: A novel ENU-induced mutation in the mouse led to nuclear and cortical opacity of the eye lens at 5 weeks postnatal. This cataract phenotype was similar to that of the zonular-pulverulent type of human cataract. Crystallin proteins and gap-junction genes have relations to the formation of cataract. Conclusions: Together, the results suggest that various proteins affect the formation and specific phenotypes of ENU-induced cataract mouse.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationCurrent Eye Research, 2009, v. 34 n. 5, p. 360-368 [How to Cite?]
DOI: http://dx.doi.org/10.1080/02713680902843047
 
dc.identifier.doihttp://dx.doi.org/10.1080/02713680902843047
 
dc.identifier.epage368
 
dc.identifier.issn0271-3683
2013 Impact Factor: 1.663
2013 SCImago Journal Rankings: 0.977
 
dc.identifier.issue5
 
dc.identifier.pmid19401879
 
dc.identifier.scopuseid_2-s2.0-67649649885
 
dc.identifier.spage360
 
dc.identifier.urihttp://hdl.handle.net/10722/169560
 
dc.identifier.volume34
 
dc.languageeng
 
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/02713683.asp
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofCurrent Eye Research
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshCataract - Chemically Induced - Genetics - Metabolism - Pathology
 
dc.subject.meshConnexins - Genetics
 
dc.subject.meshCrystallins - Genetics - Metabolism
 
dc.subject.meshDna Mutational Analysis
 
dc.subject.meshElectrophoresis, Gel, Two-Dimensional
 
dc.subject.meshEthylnitrosourea
 
dc.subject.meshEye Proteins - Genetics
 
dc.subject.meshMale
 
dc.subject.meshMice
 
dc.subject.meshMice, Inbred Balb C
 
dc.subject.meshPhenotype
 
dc.subject.meshProliferating Cell Nuclear Antigen - Metabolism
 
dc.subject.meshProtein Array Analysis
 
dc.subject.meshTissue Distribution
 
dc.titleCharacteristics of ethylnitrosourea-induced cataracts
 
dc.typeArticle
 
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<contributor.author>Kim, JY</contributor.author>
<contributor.author>Kim, YJ</contributor.author>
<contributor.author>Cho, JW</contributor.author>
<contributor.author>Cho, KH</contributor.author>
<contributor.author>Song, CW</contributor.author>
<contributor.author>Jung, HS</contributor.author>
<date.accessioned>2012-10-25T04:52:52Z</date.accessioned>
<date.available>2012-10-25T04:52:52Z</date.available>
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<subject.mesh>Ethylnitrosourea</subject.mesh>
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Author Affiliations
  1. Yonsei University
  2. Kyungpook National University
  3. Korea Research Institute Chemical Technology