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Article: Differential expression and localization of connexins 26 and 43 in the rat gingival epithelium

TitleDifferential expression and localization of connexins 26 and 43 in the rat gingival epithelium
Authors
Issue Date2008
Citation
Archives Of Histology And Cytology, 2008, v. 71 n. 3, p. 147-154 How to Cite?
AbstractWe investigated the expression and localization of connexins (CX) 26 and 43 in the rat gingival epithelium. RT-PCR analysis revealed CX26 gene expression in both the upper and lower layers of the gingival epithelium and in the total epithelial layer, whereas CX43 gene expression was limited to the lower layer and the total epithelial layer. Immunoreactivity for CX43 was observed in the membranes of adjacent cells from the basal layer to the middle of the prickle cell layer, while immunoreactivity for CX26 was observed in the granular cell layer and lower part of the squamous cell layer. Merged images revealed the co-localization of CX26 and CX43 in the middle of the prickle cell layer. By immuno-electron microscopy, gap junctions appeared curved, hemi-circular, or annular within the cytoplasm, and gold particles indicating the presence of CX43 were localized at the outer edges of these cytoplasmic formations. These results suggest that CX43 is associated with the regulation of cell proliferation and that increased CX26 expression is associated with differentiation of keratinocytes. Thus, degradation of CX43 is considered to play an essential role in differentiation of the rat gingival epithelium.
Persistent Identifierhttp://hdl.handle.net/10722/169557
ISSN
2013 Impact Factor: -999.999
2015 SCImago Journal Rankings: 0.104
References

 

DC FieldValueLanguage
dc.contributor.authorMuramatsu, Ten_US
dc.contributor.authorUekusa, Ten_US
dc.contributor.authorMasaoka, Ten_US
dc.contributor.authorSaitoh, Men_US
dc.contributor.authorHashimoto, Sen_US
dc.contributor.authorAbiko, Yen_US
dc.contributor.authorJung, HSen_US
dc.contributor.authorShimono, Men_US
dc.date.accessioned2012-10-25T04:52:51Z-
dc.date.available2012-10-25T04:52:51Z-
dc.date.issued2008en_US
dc.identifier.citationArchives Of Histology And Cytology, 2008, v. 71 n. 3, p. 147-154en_US
dc.identifier.issn0914-9465en_US
dc.identifier.urihttp://hdl.handle.net/10722/169557-
dc.description.abstractWe investigated the expression and localization of connexins (CX) 26 and 43 in the rat gingival epithelium. RT-PCR analysis revealed CX26 gene expression in both the upper and lower layers of the gingival epithelium and in the total epithelial layer, whereas CX43 gene expression was limited to the lower layer and the total epithelial layer. Immunoreactivity for CX43 was observed in the membranes of adjacent cells from the basal layer to the middle of the prickle cell layer, while immunoreactivity for CX26 was observed in the granular cell layer and lower part of the squamous cell layer. Merged images revealed the co-localization of CX26 and CX43 in the middle of the prickle cell layer. By immuno-electron microscopy, gap junctions appeared curved, hemi-circular, or annular within the cytoplasm, and gold particles indicating the presence of CX43 were localized at the outer edges of these cytoplasmic formations. These results suggest that CX43 is associated with the regulation of cell proliferation and that increased CX26 expression is associated with differentiation of keratinocytes. Thus, degradation of CX43 is considered to play an essential role in differentiation of the rat gingival epithelium.en_US
dc.languageengen_US
dc.relation.ispartofArchives of Histology and Cytologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Proliferationen_US
dc.subject.meshConnexin 43 - Analysis - Genetics - Metabolismen_US
dc.subject.meshConnexins - Analysis - Genetics - Metabolismen_US
dc.subject.meshEpithelium - Metabolism - Ultrastructureen_US
dc.subject.meshFluorescent Antibody Techniqueen_US
dc.subject.meshGap Junctions - Metabolism - Ultrastructureen_US
dc.subject.meshGingiva - Metabolismen_US
dc.subject.meshKeratinocytes - Cytology - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMicroscopy, Immunoelectronen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.titleDifferential expression and localization of connexins 26 and 43 in the rat gingival epitheliumen_US
dc.typeArticleen_US
dc.identifier.emailJung, HS: hsjung@yuhs.acen_US
dc.identifier.authorityJung, HS=rp01683en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1679/aohc.71.147en_US
dc.identifier.pmid19194037-
dc.identifier.scopuseid_2-s2.0-59949105217en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-59949105217&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume71en_US
dc.identifier.issue3en_US
dc.identifier.spage147en_US
dc.identifier.epage154en_US
dc.identifier.scopusauthoridMuramatsu, T=7402581093en_US
dc.identifier.scopusauthoridUekusa, T=23398581700en_US
dc.identifier.scopusauthoridMasaoka, T=35170444200en_US
dc.identifier.scopusauthoridSaitoh, M=7201721726en_US
dc.identifier.scopusauthoridHashimoto, S=35556628400en_US
dc.identifier.scopusauthoridAbiko, Y=7102979247en_US
dc.identifier.scopusauthoridJung, HS=7403030195en_US
dc.identifier.scopusauthoridShimono, M=7006406232en_US

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