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Article: Involvement of laminin and integrins in adhesion and migration of junctional epithelium cells

TitleInvolvement of laminin and integrins in adhesion and migration of junctional epithelium cells
Authors
Issue Date2009
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1
Citation
Journal Of Periodontal Research, 2009, v. 44 n. 1, p. 13-20 How to Cite?
AbstractBackground and Objective: The junctional epithelium attaches to the enamel surface with hemidesmosomes (of which laminin-5 and integrin- α6β4 are the main components) in the internal basal lamina. Laminin-5 is also involved in cell motility with integrin-α3β1, although their functions have not yet been clarified. The purpose of this study was to determine the functions of those adhesive components between the tooth and the junctional epithelium during cell migration. Because an idea has been proposed that directly attached to tooth cells (DAT cells) may not contribute to cell migration, 5-bromo-2-deoxyuridine staining was performed to confirm cell migration. Material and Methods: We investigated laminin-γ2 (contained only in laminin-5), integrin-β4 (involved in cell-extracellular matrix contact) and integrin-α3 (inducing cell migration) in the junctional epithelium, oral gingival epithelium and gingival sulcus epithelium of 6-wk-old ICR mice using laser microdissection, quantitative real-time reverse transcription-polymerase chain reaction, immunofluorescence and 5-bromo-2-deoxyuridine staining. Results: Laminin and integrins were clearly immunolocalized in the basal lamina of all epithelium. Quantitative analysis of laminin and integrin mRNAs by laser microdissection showed that they were more highly expressed in DAT cells than in basal cells in the oral gingival epithelium. In particular, a 12-fold higher expression of laminin-5 was observed in the junctional epithelium compared with the oral gingival epithelium. 5-Bromo-2-deoxyuridine staining showed rapid coronal migration of DAT cells. Conclusion: These results suggest that the abundant expression of laminin-5 and integrin-α6β4 is involved in the attachment of DAT cells to teeth by hemidesmosomes. Abundant expression of laminin-5 and integrin-α3β1 might assist in DAT cell migration, confirmed by 5-bromo-2-deoxyuridine staining during the turnover of junctional epithelium. © 2008 The Authors.
Persistent Identifierhttp://hdl.handle.net/10722/169555
ISSN
2015 Impact Factor: 2.474
2015 SCImago Journal Rankings: 0.932
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKinumatsu, Ten_US
dc.contributor.authorHashimoto, Sen_US
dc.contributor.authorMuramatsu, Ten_US
dc.contributor.authorSasaki, Hen_US
dc.contributor.authorJung, HSen_US
dc.contributor.authorYamada, Sen_US
dc.contributor.authorShimono, Men_US
dc.date.accessioned2012-10-25T04:52:50Z-
dc.date.available2012-10-25T04:52:50Z-
dc.date.issued2009en_US
dc.identifier.citationJournal Of Periodontal Research, 2009, v. 44 n. 1, p. 13-20en_US
dc.identifier.issn0022-3484en_US
dc.identifier.urihttp://hdl.handle.net/10722/169555-
dc.description.abstractBackground and Objective: The junctional epithelium attaches to the enamel surface with hemidesmosomes (of which laminin-5 and integrin- α6β4 are the main components) in the internal basal lamina. Laminin-5 is also involved in cell motility with integrin-α3β1, although their functions have not yet been clarified. The purpose of this study was to determine the functions of those adhesive components between the tooth and the junctional epithelium during cell migration. Because an idea has been proposed that directly attached to tooth cells (DAT cells) may not contribute to cell migration, 5-bromo-2-deoxyuridine staining was performed to confirm cell migration. Material and Methods: We investigated laminin-γ2 (contained only in laminin-5), integrin-β4 (involved in cell-extracellular matrix contact) and integrin-α3 (inducing cell migration) in the junctional epithelium, oral gingival epithelium and gingival sulcus epithelium of 6-wk-old ICR mice using laser microdissection, quantitative real-time reverse transcription-polymerase chain reaction, immunofluorescence and 5-bromo-2-deoxyuridine staining. Results: Laminin and integrins were clearly immunolocalized in the basal lamina of all epithelium. Quantitative analysis of laminin and integrin mRNAs by laser microdissection showed that they were more highly expressed in DAT cells than in basal cells in the oral gingival epithelium. In particular, a 12-fold higher expression of laminin-5 was observed in the junctional epithelium compared with the oral gingival epithelium. 5-Bromo-2-deoxyuridine staining showed rapid coronal migration of DAT cells. Conclusion: These results suggest that the abundant expression of laminin-5 and integrin-α6β4 is involved in the attachment of DAT cells to teeth by hemidesmosomes. Abundant expression of laminin-5 and integrin-α3β1 might assist in DAT cell migration, confirmed by 5-bromo-2-deoxyuridine staining during the turnover of junctional epithelium. © 2008 The Authors.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1en_US
dc.relation.ispartofJournal of Periodontal Researchen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntimetabolites - Diagnostic Useen_US
dc.subject.meshBromodeoxyuridine - Diagnostic Useen_US
dc.subject.meshCell Adhesion - Physiologyen_US
dc.subject.meshCell Adhesion Molecules - Analysisen_US
dc.subject.meshCell Movement - Physiologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshEpithelial Attachment - Cytologyen_US
dc.subject.meshEpithelial Cells - Cytologyen_US
dc.subject.meshFluorescent Antibody Technique, Directen_US
dc.subject.meshGingiva - Cytologyen_US
dc.subject.meshHemidesmosomes - Ultrastructureen_US
dc.subject.meshIntegrin Alpha3 - Analysisen_US
dc.subject.meshIntegrin Alpha3beta1 - Analysisen_US
dc.subject.meshIntegrin Alpha6beta4 - Analysisen_US
dc.subject.meshIntegrin Beta4 - Analysisen_US
dc.subject.meshLaminin - Analysisen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Icren_US
dc.subject.meshMicrodissectionen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.titleInvolvement of laminin and integrins in adhesion and migration of junctional epithelium cellsen_US
dc.typeArticleen_US
dc.identifier.emailJung, HS: hsjung@yuhs.acen_US
dc.identifier.authorityJung, HS=rp01683en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1600-0765.2007.01036.xen_US
dc.identifier.pmid18973537-
dc.identifier.scopuseid_2-s2.0-58149330370en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-58149330370&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume44en_US
dc.identifier.issue1en_US
dc.identifier.spage13en_US
dc.identifier.epage20en_US
dc.identifier.isiWOS:000262306300002-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKinumatsu, T=6507201640en_US
dc.identifier.scopusauthoridHashimoto, S=35556628400en_US
dc.identifier.scopusauthoridMuramatsu, T=7402581093en_US
dc.identifier.scopusauthoridSasaki, H=14038203400en_US
dc.identifier.scopusauthoridJung, HS=7403030195en_US
dc.identifier.scopusauthoridYamada, S=7404921517en_US
dc.identifier.scopusauthoridShimono, M=19536788600en_US
dc.identifier.citeulike3895620-

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