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Article: Morphological evidence of basal keratinocyte migration during the re-epithelialization process

TitleMorphological evidence of basal keratinocyte migration during the re-epithelialization process
Authors
Issue Date2008
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00418/index.htm
Citation
Histochemistry And Cell Biology, 2008, v. 130 n. 6, p. 1165-1175 How to Cite?
Abstract
The regeneration of wounded stratified epithelium is accomplished via the migration of keratinocytes from the margins of the wound. However, the process of keratinocyte migration on the wound surface and the role of epithelial stem cells during re-epithelialization remain to be elucidated. Therefore, we administered BrdU to embryonic mice and generated epithelial defects on the buccal mucosa of these mice at two weeks after birth, using CO2 laser irradiation, with which we removed the entire thickness of the epithelium. In the unwounded epithelium, cytokeratin 14, p63, and BrdU were localized within the basal layer of the epithelium, but the majority of cells within the regenerated epithelium were immunopositive for these proteins. PCNA-negative and BrdU-positive basal keratinocytes, which evidence a slow cell cycle, were localized solely within the basal layer of the unwound epithelium facing the tips of dermal papillae. After laser irradiation, these basal keratinocytes facing the tips of the papillae evidenced positive immunoreactivity for PCNA, in addition to BrdU. These results indicate that epithelial stem cells of oral mucosa may be localized in the basal layer of the epithelium facing the tips of dermal papillae, and may migrate laterally with other basal keratinocytes in response to external stimuli. © 2008 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/169553
ISSN
2013 Impact Factor: 2.927
ISI Accession Number ID
References

 

Author Affiliations
  1. Matsumoto Dental University
  2. Yonsei University College of Medicine
  3. Tokyo Dental College
DC FieldValueLanguage
dc.contributor.authorHosoya, Aen_US
dc.contributor.authorLee, JMen_US
dc.contributor.authorCho, SWen_US
dc.contributor.authorKim, JYen_US
dc.contributor.authorShinozaki, Nen_US
dc.contributor.authorShibahara, Ten_US
dc.contributor.authorShimono, Men_US
dc.contributor.authorJung, HSen_US
dc.date.accessioned2012-10-25T04:52:48Z-
dc.date.available2012-10-25T04:52:48Z-
dc.date.issued2008en_US
dc.identifier.citationHistochemistry And Cell Biology, 2008, v. 130 n. 6, p. 1165-1175en_US
dc.identifier.issn0948-6143en_US
dc.identifier.urihttp://hdl.handle.net/10722/169553-
dc.description.abstractThe regeneration of wounded stratified epithelium is accomplished via the migration of keratinocytes from the margins of the wound. However, the process of keratinocyte migration on the wound surface and the role of epithelial stem cells during re-epithelialization remain to be elucidated. Therefore, we administered BrdU to embryonic mice and generated epithelial defects on the buccal mucosa of these mice at two weeks after birth, using CO2 laser irradiation, with which we removed the entire thickness of the epithelium. In the unwounded epithelium, cytokeratin 14, p63, and BrdU were localized within the basal layer of the epithelium, but the majority of cells within the regenerated epithelium were immunopositive for these proteins. PCNA-negative and BrdU-positive basal keratinocytes, which evidence a slow cell cycle, were localized solely within the basal layer of the unwound epithelium facing the tips of dermal papillae. After laser irradiation, these basal keratinocytes facing the tips of the papillae evidenced positive immunoreactivity for PCNA, in addition to BrdU. These results indicate that epithelial stem cells of oral mucosa may be localized in the basal layer of the epithelium facing the tips of dermal papillae, and may migrate laterally with other basal keratinocytes in response to external stimuli. © 2008 Springer-Verlag.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00418/index.htmen_US
dc.relation.ispartofHistochemistry and Cell Biologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Newbornen_US
dc.subject.meshBromodeoxyuridine - Metabolismen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Movementen_US
dc.subject.meshEpithelial Cells - Metabolism - Pathology - Radiation Effectsen_US
dc.subject.meshKeratin-14 - Metabolismen_US
dc.subject.meshKeratin-5 - Metabolismen_US
dc.subject.meshKeratinocytes - Metabolism - Pathology - Radiation Effectsen_US
dc.subject.meshLasers, Gasen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Icren_US
dc.subject.meshMouth Mucosa - Metabolism - Pathology - Radiation Effectsen_US
dc.subject.meshPhosphoproteins - Metabolismen_US
dc.subject.meshProliferating Cell Nuclear Antigen - Metabolismen_US
dc.subject.meshStem Cells - Metabolism - Pathology - Radiation Effectsen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTrans-Activators - Metabolismen_US
dc.subject.meshWound Healingen_US
dc.titleMorphological evidence of basal keratinocyte migration during the re-epithelialization processen_US
dc.typeArticleen_US
dc.identifier.emailJung, HS: hsjung@yuhs.acen_US
dc.identifier.authorityJung, HS=rp01683en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00418-008-0499-3en_US
dc.identifier.pmid18773217en_US
dc.identifier.scopuseid_2-s2.0-56749095722en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-56749095722&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume130en_US
dc.identifier.issue6en_US
dc.identifier.spage1165en_US
dc.identifier.epage1175en_US
dc.identifier.isiWOS:000261956400010-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridHosoya, A=8651007100en_US
dc.identifier.scopusauthoridLee, JM=25622544900en_US
dc.identifier.scopusauthoridCho, SW=32967447200en_US
dc.identifier.scopusauthoridKim, JY=7601381285en_US
dc.identifier.scopusauthoridShinozaki, N=7006255698en_US
dc.identifier.scopusauthoridShibahara, T=7102434258en_US
dc.identifier.scopusauthoridShimono, M=7006406232en_US
dc.identifier.scopusauthoridJung, HS=7403030195en_US

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