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Article: Morphological evidence of basal keratinocyte migration during the re-epithelialization process
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TitleMorphological evidence of basal keratinocyte migration during the re-epithelialization process
 
AuthorsHosoya, A2 1
Lee, JM2
Cho, SW2
Kim, JY2
Shinozaki, N3
Shibahara, T3
Shimono, M3
Jung, HS2
 
Issue Date2008
 
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00418/index.htm
 
CitationHistochemistry And Cell Biology, 2008, v. 130 n. 6, p. 1165-1175 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00418-008-0499-3
 
AbstractThe regeneration of wounded stratified epithelium is accomplished via the migration of keratinocytes from the margins of the wound. However, the process of keratinocyte migration on the wound surface and the role of epithelial stem cells during re-epithelialization remain to be elucidated. Therefore, we administered BrdU to embryonic mice and generated epithelial defects on the buccal mucosa of these mice at two weeks after birth, using CO2 laser irradiation, with which we removed the entire thickness of the epithelium. In the unwounded epithelium, cytokeratin 14, p63, and BrdU were localized within the basal layer of the epithelium, but the majority of cells within the regenerated epithelium were immunopositive for these proteins. PCNA-negative and BrdU-positive basal keratinocytes, which evidence a slow cell cycle, were localized solely within the basal layer of the unwound epithelium facing the tips of dermal papillae. After laser irradiation, these basal keratinocytes facing the tips of the papillae evidenced positive immunoreactivity for PCNA, in addition to BrdU. These results indicate that epithelial stem cells of oral mucosa may be localized in the basal layer of the epithelium facing the tips of dermal papillae, and may migrate laterally with other basal keratinocytes in response to external stimuli. © 2008 Springer-Verlag.
 
ISSN0948-6143
2013 Impact Factor: 2.927
 
DOIhttp://dx.doi.org/10.1007/s00418-008-0499-3
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorHosoya, A
 
dc.contributor.authorLee, JM
 
dc.contributor.authorCho, SW
 
dc.contributor.authorKim, JY
 
dc.contributor.authorShinozaki, N
 
dc.contributor.authorShibahara, T
 
dc.contributor.authorShimono, M
 
dc.contributor.authorJung, HS
 
dc.date.accessioned2012-10-25T04:52:48Z
 
dc.date.available2012-10-25T04:52:48Z
 
dc.date.issued2008
 
dc.description.abstractThe regeneration of wounded stratified epithelium is accomplished via the migration of keratinocytes from the margins of the wound. However, the process of keratinocyte migration on the wound surface and the role of epithelial stem cells during re-epithelialization remain to be elucidated. Therefore, we administered BrdU to embryonic mice and generated epithelial defects on the buccal mucosa of these mice at two weeks after birth, using CO2 laser irradiation, with which we removed the entire thickness of the epithelium. In the unwounded epithelium, cytokeratin 14, p63, and BrdU were localized within the basal layer of the epithelium, but the majority of cells within the regenerated epithelium were immunopositive for these proteins. PCNA-negative and BrdU-positive basal keratinocytes, which evidence a slow cell cycle, were localized solely within the basal layer of the unwound epithelium facing the tips of dermal papillae. After laser irradiation, these basal keratinocytes facing the tips of the papillae evidenced positive immunoreactivity for PCNA, in addition to BrdU. These results indicate that epithelial stem cells of oral mucosa may be localized in the basal layer of the epithelium facing the tips of dermal papillae, and may migrate laterally with other basal keratinocytes in response to external stimuli. © 2008 Springer-Verlag.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationHistochemistry And Cell Biology, 2008, v. 130 n. 6, p. 1165-1175 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00418-008-0499-3
 
dc.identifier.doihttp://dx.doi.org/10.1007/s00418-008-0499-3
 
dc.identifier.epage1175
 
dc.identifier.issn0948-6143
2013 Impact Factor: 2.927
 
dc.identifier.issue6
 
dc.identifier.pmid18773217
 
dc.identifier.scopuseid_2-s2.0-56749095722
 
dc.identifier.spage1165
 
dc.identifier.urihttp://hdl.handle.net/10722/169553
 
dc.identifier.volume130
 
dc.languageeng
 
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00418/index.htm
 
dc.publisher.placeGermany
 
dc.relation.ispartofHistochemistry and Cell Biology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshAnimals, Newborn
 
dc.subject.meshBromodeoxyuridine - Metabolism
 
dc.subject.meshCell Differentiation
 
dc.subject.meshCell Movement
 
dc.subject.meshEpithelial Cells - Metabolism - Pathology - Radiation Effects
 
dc.subject.meshKeratin-14 - Metabolism
 
dc.subject.meshKeratin-5 - Metabolism
 
dc.subject.meshKeratinocytes - Metabolism - Pathology - Radiation Effects
 
dc.subject.meshLasers, Gas
 
dc.subject.meshMice
 
dc.subject.meshMice, Inbred Icr
 
dc.subject.meshMouth Mucosa - Metabolism - Pathology - Radiation Effects
 
dc.subject.meshPhosphoproteins - Metabolism
 
dc.subject.meshProliferating Cell Nuclear Antigen - Metabolism
 
dc.subject.meshStem Cells - Metabolism - Pathology - Radiation Effects
 
dc.subject.meshTime Factors
 
dc.subject.meshTrans-Activators - Metabolism
 
dc.subject.meshWound Healing
 
dc.titleMorphological evidence of basal keratinocyte migration during the re-epithelialization process
 
dc.typeArticle
 
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<contributor.author>Shinozaki, N</contributor.author>
<contributor.author>Shibahara, T</contributor.author>
<contributor.author>Shimono, M</contributor.author>
<contributor.author>Jung, HS</contributor.author>
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<description.abstract>The regeneration of wounded stratified epithelium is accomplished via the migration of keratinocytes from the margins of the wound. However, the process of keratinocyte migration on the wound surface and the role of epithelial stem cells during re-epithelialization remain to be elucidated. Therefore, we administered BrdU to embryonic mice and generated epithelial defects on the buccal mucosa of these mice at two weeks after birth, using CO2 laser irradiation, with which we removed the entire thickness of the epithelium. In the unwounded epithelium, cytokeratin 14, p63, and BrdU were localized within the basal layer of the epithelium, but the majority of cells within the regenerated epithelium were immunopositive for these proteins. PCNA-negative and BrdU-positive basal keratinocytes, which evidence a slow cell cycle, were localized solely within the basal layer of the unwound epithelium facing the tips of dermal papillae. After laser irradiation, these basal keratinocytes facing the tips of the papillae evidenced positive immunoreactivity for PCNA, in addition to BrdU. These results indicate that epithelial stem cells of oral mucosa may be localized in the basal layer of the epithelium facing the tips of dermal papillae, and may migrate laterally with other basal keratinocytes in response to external stimuli. &#169; 2008 Springer-Verlag.</description.abstract>
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Author Affiliations
  1. Matsumoto Dental University
  2. Yonsei University College of Medicine
  3. Tokyo Dental College