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Article: BMP4 signaling regulates formation of Hertwig's epithelial root sheath during tooth root development

TitleBMP4 signaling regulates formation of Hertwig's epithelial root sheath during tooth root development
Authors
Issue Date2008
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm
Citation
Cell And Tissue Research, 2008, v. 333 n. 3, p. 503-509 How to Cite?
AbstractAlthough Hertwig's epithelial root sheath (HERS) performs an important function in the formation of the tooth root, the developmental mechanisms that control HERS growth and differentiation remain to be thoroughly elucidated. Bone morphogenetic protein 4 (BMP4), which is secreted by mesenchymal cells, acts on the dental epithelium as a regulator of cell differentiation during crown formation. In an effort to determine whether BMP4 specifically regulates the development of HERS in the dental epithelium, we assessed the localizations of BMP4, BMP receptor-IB (BMPR-IB), and BMPR-II during molar root formation in the mouse. HERS cells were shown to express BMPR-IB and BMPR-II. BMP4-positive cells were detected densely in the dental papillae around HERS, thereby suggesting that BMP4 participated in HERS formation. Beads soaked in BMP4, NOGGIN, or phosphate-buffered saline (PBS) were implanted into the pulp cavity under culture conditions, and the length of HERS was evaluated with regard to the proliferating cells. After 12 h, both groups exhibited a similar HERS developmental pattern, with the length and shape of HERS bearing a close resemblance to one another. However, after 48 h, the observed HERS elongation was significantly shorter in the BMP4-treated group. In addition, proliferative cell nuclear antigens were detectable only in the NOGGIN- and PBS-treated groups. These findings demonstrate that mesenchymally expressed BMP4 regulates HERS development by preventing elongation and maintaining cell proliferation. BMP4 may, therefore, prove useful as a root-formation regulatory agent in a variety of tissue-engineering applications. © 2008 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/169548
ISSN
2014 Impact Factor: 3.565
2014 SCImago Journal Rankings: 1.293
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHosoya, Aen_US
dc.contributor.authorKim, JYen_US
dc.contributor.authorCho, SWen_US
dc.contributor.authorJung, HSen_US
dc.date.accessioned2012-10-25T04:52:46Z-
dc.date.available2012-10-25T04:52:46Z-
dc.date.issued2008en_US
dc.identifier.citationCell And Tissue Research, 2008, v. 333 n. 3, p. 503-509en_US
dc.identifier.issn0302-766Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/169548-
dc.description.abstractAlthough Hertwig's epithelial root sheath (HERS) performs an important function in the formation of the tooth root, the developmental mechanisms that control HERS growth and differentiation remain to be thoroughly elucidated. Bone morphogenetic protein 4 (BMP4), which is secreted by mesenchymal cells, acts on the dental epithelium as a regulator of cell differentiation during crown formation. In an effort to determine whether BMP4 specifically regulates the development of HERS in the dental epithelium, we assessed the localizations of BMP4, BMP receptor-IB (BMPR-IB), and BMPR-II during molar root formation in the mouse. HERS cells were shown to express BMPR-IB and BMPR-II. BMP4-positive cells were detected densely in the dental papillae around HERS, thereby suggesting that BMP4 participated in HERS formation. Beads soaked in BMP4, NOGGIN, or phosphate-buffered saline (PBS) were implanted into the pulp cavity under culture conditions, and the length of HERS was evaluated with regard to the proliferating cells. After 12 h, both groups exhibited a similar HERS developmental pattern, with the length and shape of HERS bearing a close resemblance to one another. However, after 48 h, the observed HERS elongation was significantly shorter in the BMP4-treated group. In addition, proliferative cell nuclear antigens were detectable only in the NOGGIN- and PBS-treated groups. These findings demonstrate that mesenchymally expressed BMP4 regulates HERS development by preventing elongation and maintaining cell proliferation. BMP4 may, therefore, prove useful as a root-formation regulatory agent in a variety of tissue-engineering applications. © 2008 Springer-Verlag.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htmen_US
dc.relation.ispartofCell and Tissue Researchen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBone Morphogenetic Protein 4 - Genetics - Pharmacology - Physiologyen_US
dc.subject.meshBone Morphogenetic Protein Receptors - Metabolismen_US
dc.subject.meshCarrier Proteins - Genetics - Pharmacology - Physiologyen_US
dc.subject.meshCell Proliferation - Drug Effectsen_US
dc.subject.meshEpithelial Cells - Cytology - Drug Effects - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Icren_US
dc.subject.meshOdontoblasts - Cytology - Metabolismen_US
dc.subject.meshOrgan Culture Techniquesen_US
dc.subject.meshRecombinant Proteins - Genetics - Pharmacologyen_US
dc.subject.meshSignal Transduction - Physiologyen_US
dc.subject.meshTooth Root - Cytology - Drug Effects - Growth & Developmenten_US
dc.titleBMP4 signaling regulates formation of Hertwig's epithelial root sheath during tooth root developmenten_US
dc.typeArticleen_US
dc.identifier.emailJung, HS: hsjung@yuhs.acen_US
dc.identifier.authorityJung, HS=rp01683en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00441-008-0655-zen_US
dc.identifier.pmid18629540en_US
dc.identifier.scopuseid_2-s2.0-49749135492en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-49749135492&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume333en_US
dc.identifier.issue3en_US
dc.identifier.spage503en_US
dc.identifier.epage509en_US
dc.identifier.isiWOS:000258528200014-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridHosoya, A=8651007100en_US
dc.identifier.scopusauthoridKim, JY=7601381285en_US
dc.identifier.scopusauthoridCho, SW=32967447200en_US
dc.identifier.scopusauthoridJung, HS=7403030195en_US
dc.identifier.citeulike3174168-

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