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Article: BMP4 signaling regulates formation of Hertwig's epithelial root sheath during tooth root development
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TitleBMP4 signaling regulates formation of Hertwig's epithelial root sheath during tooth root development
 
AuthorsHosoya, A2 1
Kim, JY2
Cho, SW2
Jung, HS2
 
Issue Date2008
 
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm
 
CitationCell And Tissue Research, 2008, v. 333 n. 3, p. 503-509 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00441-008-0655-z
 
AbstractAlthough Hertwig's epithelial root sheath (HERS) performs an important function in the formation of the tooth root, the developmental mechanisms that control HERS growth and differentiation remain to be thoroughly elucidated. Bone morphogenetic protein 4 (BMP4), which is secreted by mesenchymal cells, acts on the dental epithelium as a regulator of cell differentiation during crown formation. In an effort to determine whether BMP4 specifically regulates the development of HERS in the dental epithelium, we assessed the localizations of BMP4, BMP receptor-IB (BMPR-IB), and BMPR-II during molar root formation in the mouse. HERS cells were shown to express BMPR-IB and BMPR-II. BMP4-positive cells were detected densely in the dental papillae around HERS, thereby suggesting that BMP4 participated in HERS formation. Beads soaked in BMP4, NOGGIN, or phosphate-buffered saline (PBS) were implanted into the pulp cavity under culture conditions, and the length of HERS was evaluated with regard to the proliferating cells. After 12 h, both groups exhibited a similar HERS developmental pattern, with the length and shape of HERS bearing a close resemblance to one another. However, after 48 h, the observed HERS elongation was significantly shorter in the BMP4-treated group. In addition, proliferative cell nuclear antigens were detectable only in the NOGGIN- and PBS-treated groups. These findings demonstrate that mesenchymally expressed BMP4 regulates HERS development by preventing elongation and maintaining cell proliferation. BMP4 may, therefore, prove useful as a root-formation regulatory agent in a variety of tissue-engineering applications. © 2008 Springer-Verlag.
 
ISSN0302-766X
2013 Impact Factor: 3.333
 
DOIhttp://dx.doi.org/10.1007/s00441-008-0655-z
 
ISI Accession Number IDWOS:000258528200014
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorHosoya, A
 
dc.contributor.authorKim, JY
 
dc.contributor.authorCho, SW
 
dc.contributor.authorJung, HS
 
dc.date.accessioned2012-10-25T04:52:46Z
 
dc.date.available2012-10-25T04:52:46Z
 
dc.date.issued2008
 
dc.description.abstractAlthough Hertwig's epithelial root sheath (HERS) performs an important function in the formation of the tooth root, the developmental mechanisms that control HERS growth and differentiation remain to be thoroughly elucidated. Bone morphogenetic protein 4 (BMP4), which is secreted by mesenchymal cells, acts on the dental epithelium as a regulator of cell differentiation during crown formation. In an effort to determine whether BMP4 specifically regulates the development of HERS in the dental epithelium, we assessed the localizations of BMP4, BMP receptor-IB (BMPR-IB), and BMPR-II during molar root formation in the mouse. HERS cells were shown to express BMPR-IB and BMPR-II. BMP4-positive cells were detected densely in the dental papillae around HERS, thereby suggesting that BMP4 participated in HERS formation. Beads soaked in BMP4, NOGGIN, or phosphate-buffered saline (PBS) were implanted into the pulp cavity under culture conditions, and the length of HERS was evaluated with regard to the proliferating cells. After 12 h, both groups exhibited a similar HERS developmental pattern, with the length and shape of HERS bearing a close resemblance to one another. However, after 48 h, the observed HERS elongation was significantly shorter in the BMP4-treated group. In addition, proliferative cell nuclear antigens were detectable only in the NOGGIN- and PBS-treated groups. These findings demonstrate that mesenchymally expressed BMP4 regulates HERS development by preventing elongation and maintaining cell proliferation. BMP4 may, therefore, prove useful as a root-formation regulatory agent in a variety of tissue-engineering applications. © 2008 Springer-Verlag.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationCell And Tissue Research, 2008, v. 333 n. 3, p. 503-509 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00441-008-0655-z
 
dc.identifier.citeulike3174168
 
dc.identifier.doihttp://dx.doi.org/10.1007/s00441-008-0655-z
 
dc.identifier.epage509
 
dc.identifier.isiWOS:000258528200014
 
dc.identifier.issn0302-766X
2013 Impact Factor: 3.333
 
dc.identifier.issue3
 
dc.identifier.pmid18629540
 
dc.identifier.scopuseid_2-s2.0-49749135492
 
dc.identifier.spage503
 
dc.identifier.urihttp://hdl.handle.net/10722/169548
 
dc.identifier.volume333
 
dc.languageeng
 
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm
 
dc.publisher.placeGermany
 
dc.relation.ispartofCell and Tissue Research
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshBone Morphogenetic Protein 4 - Genetics - Pharmacology - Physiology
 
dc.subject.meshBone Morphogenetic Protein Receptors - Metabolism
 
dc.subject.meshCarrier Proteins - Genetics - Pharmacology - Physiology
 
dc.subject.meshCell Proliferation - Drug Effects
 
dc.subject.meshEpithelial Cells - Cytology - Drug Effects - Metabolism
 
dc.subject.meshHumans
 
dc.subject.meshImmunohistochemistry
 
dc.subject.meshMice
 
dc.subject.meshMice, Inbred Icr
 
dc.subject.meshOdontoblasts - Cytology - Metabolism
 
dc.subject.meshOrgan Culture Techniques
 
dc.subject.meshRecombinant Proteins - Genetics - Pharmacology
 
dc.subject.meshSignal Transduction - Physiology
 
dc.subject.meshTooth Root - Cytology - Drug Effects - Growth & Development
 
dc.titleBMP4 signaling regulates formation of Hertwig's epithelial root sheath during tooth root development
 
dc.typeArticle
 
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Author Affiliations
  1. Matsumoto Dental University
  2. Yonsei University College of Medicine