Article: An MTT-based method for quantification of periodontal ligament cell viability

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TitleAn MTT-based method for quantification of periodontal ligament cell viability
AuthorsKim, E1
Jeon, IS1
Kim, JW1
Kim, J1
Jung, HS1
Lee, SJ1
Issue Date2007
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1354-523X&site=1
CitationOral Diseases, 2007, v. 13 n. 5, p. 495-499 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1601-0825.2006.01328.x
AbstractObjective: Survival of periodontal ligament cells is crucial issue after tooth replantation. To understand this matter, we introduced MTT assay, which can be used as a tool for measuring the viability of periodontal ligament cells from extracted rat molars. Materials and methods: The maxillary molars of 4-week-old Sprague-Dawley white female rats were used. Ten teeth of each immediate, 1 h Viaspan and 1 h dry were processed for MTT evaluation. Another 10 teeth from each group were treated in the same manner as above, but were replanted into their original socket. After 2 weeks, the animals were killed and the prevalence of resorption pits was evaluated. Results: In vivo MTT assay corresponded with the histological results of the resorption pits (P ≤ 0.05). The polarizing and optical microscopic findings were consistent with the in vivo MTT assay results. Conclusions: In vivo MTT measurements were consistent with the histologic observations and suggest that the in vivo MTT assay could be a tool for evaluating the viability of periodontal ligament cells directly from the extracted root surface. The advantages are shorter analysis time compared with animal or cell culture experiments, easy manipulation, clear quantification and immediate identification of the vital cells. © 2007 The Authors.
ISSN1354-523X
2011 Impact Factor: 2.495
2011 SCImago Journal Rankings: 0.168
DOIhttp://dx.doi.org/10.1111/j.1601-0825.2006.01328.x
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorKim, E
dc.contributor.authorJeon, IS
dc.contributor.authorKim, JW
dc.contributor.authorKim, J
dc.contributor.authorJung, HS
dc.contributor.authorLee, SJ
dc.date.accessioned2012-10-25T04:52:44Z
dc.date.available2012-10-25T04:52:44Z
dc.date.issued2007
dc.description.abstractObjective: Survival of periodontal ligament cells is crucial issue after tooth replantation. To understand this matter, we introduced MTT assay, which can be used as a tool for measuring the viability of periodontal ligament cells from extracted rat molars. Materials and methods: The maxillary molars of 4-week-old Sprague-Dawley white female rats were used. Ten teeth of each immediate, 1 h Viaspan and 1 h dry were processed for MTT evaluation. Another 10 teeth from each group were treated in the same manner as above, but were replanted into their original socket. After 2 weeks, the animals were killed and the prevalence of resorption pits was evaluated. Results: In vivo MTT assay corresponded with the histological results of the resorption pits (P ≤ 0.05). The polarizing and optical microscopic findings were consistent with the in vivo MTT assay results. Conclusions: In vivo MTT measurements were consistent with the histologic observations and suggest that the in vivo MTT assay could be a tool for evaluating the viability of periodontal ligament cells directly from the extracted root surface. The advantages are shorter analysis time compared with animal or cell culture experiments, easy manipulation, clear quantification and immediate identification of the vital cells. © 2007 The Authors.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationOral Diseases, 2007, v. 13 n. 5, p. 495-499 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1601-0825.2006.01328.x
dc.identifier.citeulike1558252
dc.identifier.doihttp://dx.doi.org/10.1111/j.1601-0825.2006.01328.x
dc.identifier.epage499
dc.identifier.issn1354-523X
2011 Impact Factor: 2.495
2011 SCImago Journal Rankings: 0.168
dc.identifier.issue5
dc.identifier.pmid17714353
dc.identifier.scopuseid_2-s2.0-34547850940
dc.identifier.spage495
dc.identifier.urihttp://hdl.handle.net/10722/169545
dc.identifier.volume13
dc.languageeng
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1354-523X&site=1
dc.publisher.placeUnited States
dc.relation.ispartofOral Diseases
dc.relation.referencesReferences in Scopus
dc.subject.meshAnimals
dc.subject.meshCell Survival
dc.subject.meshFemale
dc.subject.meshFormazans
dc.subject.meshIndicators And Reagents
dc.subject.meshMolar - Cytology - Pathology
dc.subject.meshPeriodontal Ligament - Cytology - Pathology
dc.subject.meshRats
dc.subject.meshRats, Sprague-Dawley
dc.subject.meshRoot Resorption - Etiology - Pathology
dc.subject.meshTooth Replantation - Adverse Effects
dc.titleAn MTT-based method for quantification of periodontal ligament cell viability
dc.typeArticle
Author Affiliations
  1. Yonsei University