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Article: An MTT-based method for quantification of periodontal ligament cell viability

TitleAn MTT-based method for quantification of periodontal ligament cell viability
Authors
Issue Date2007
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1354-523X&site=1
Citation
Oral Diseases, 2007, v. 13 n. 5, p. 495-499 How to Cite?
Abstract
Objective: Survival of periodontal ligament cells is crucial issue after tooth replantation. To understand this matter, we introduced MTT assay, which can be used as a tool for measuring the viability of periodontal ligament cells from extracted rat molars. Materials and methods: The maxillary molars of 4-week-old Sprague-Dawley white female rats were used. Ten teeth of each immediate, 1 h Viaspan and 1 h dry were processed for MTT evaluation. Another 10 teeth from each group were treated in the same manner as above, but were replanted into their original socket. After 2 weeks, the animals were killed and the prevalence of resorption pits was evaluated. Results: In vivo MTT assay corresponded with the histological results of the resorption pits (P ≤ 0.05). The polarizing and optical microscopic findings were consistent with the in vivo MTT assay results. Conclusions: In vivo MTT measurements were consistent with the histologic observations and suggest that the in vivo MTT assay could be a tool for evaluating the viability of periodontal ligament cells directly from the extracted root surface. The advantages are shorter analysis time compared with animal or cell culture experiments, easy manipulation, clear quantification and immediate identification of the vital cells. © 2007 The Authors.
Persistent Identifierhttp://hdl.handle.net/10722/169545
ISSN
2013 Impact Factor: 2.404
ISI Accession Number ID
References

 

Author Affiliations
  1. Yonsei University
DC FieldValueLanguage
dc.contributor.authorKim, Een_US
dc.contributor.authorJeon, ISen_US
dc.contributor.authorKim, JWen_US
dc.contributor.authorKim, Jen_US
dc.contributor.authorJung, HSen_US
dc.contributor.authorLee, SJen_US
dc.date.accessioned2012-10-25T04:52:44Z-
dc.date.available2012-10-25T04:52:44Z-
dc.date.issued2007en_US
dc.identifier.citationOral Diseases, 2007, v. 13 n. 5, p. 495-499en_US
dc.identifier.issn1354-523Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/169545-
dc.description.abstractObjective: Survival of periodontal ligament cells is crucial issue after tooth replantation. To understand this matter, we introduced MTT assay, which can be used as a tool for measuring the viability of periodontal ligament cells from extracted rat molars. Materials and methods: The maxillary molars of 4-week-old Sprague-Dawley white female rats were used. Ten teeth of each immediate, 1 h Viaspan and 1 h dry were processed for MTT evaluation. Another 10 teeth from each group were treated in the same manner as above, but were replanted into their original socket. After 2 weeks, the animals were killed and the prevalence of resorption pits was evaluated. Results: In vivo MTT assay corresponded with the histological results of the resorption pits (P ≤ 0.05). The polarizing and optical microscopic findings were consistent with the in vivo MTT assay results. Conclusions: In vivo MTT measurements were consistent with the histologic observations and suggest that the in vivo MTT assay could be a tool for evaluating the viability of periodontal ligament cells directly from the extracted root surface. The advantages are shorter analysis time compared with animal or cell culture experiments, easy manipulation, clear quantification and immediate identification of the vital cells. © 2007 The Authors.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1354-523X&site=1en_US
dc.relation.ispartofOral Diseasesen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Survivalen_US
dc.subject.meshFemaleen_US
dc.subject.meshFormazansen_US
dc.subject.meshIndicators And Reagentsen_US
dc.subject.meshMolar - Cytology - Pathologyen_US
dc.subject.meshPeriodontal Ligament - Cytology - Pathologyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshRoot Resorption - Etiology - Pathologyen_US
dc.subject.meshTooth Replantation - Adverse Effectsen_US
dc.titleAn MTT-based method for quantification of periodontal ligament cell viabilityen_US
dc.typeArticleen_US
dc.identifier.emailJung, HS: hsjung@yuhs.acen_US
dc.identifier.authorityJung, HS=rp01683en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1601-0825.2006.01328.xen_US
dc.identifier.pmid17714353en_US
dc.identifier.scopuseid_2-s2.0-34547850940en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34547850940&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume13en_US
dc.identifier.issue5en_US
dc.identifier.spage495en_US
dc.identifier.epage499en_US
dc.identifier.isiWOS:000249194500008-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKim, E=7404505980en_US
dc.identifier.scopusauthoridJeon, IS=36943877900en_US
dc.identifier.scopusauthoridKim, JW=8560808100en_US
dc.identifier.scopusauthoridKim, J=7601364064en_US
dc.identifier.scopusauthoridJung, HS=7403030195en_US
dc.identifier.scopusauthoridLee, SJ=36064890000en_US
dc.identifier.citeulike1558252-

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