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Article: An MTT-based method for quantification of periodontal ligament cell viability
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TitleAn MTT-based method for quantification of periodontal ligament cell viability
 
AuthorsKim, E1
Jeon, IS1
Kim, JW1
Kim, J1
Jung, HS1
Lee, SJ1
 
Issue Date2007
 
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1354-523X&site=1
 
CitationOral Diseases, 2007, v. 13 n. 5, p. 495-499 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1601-0825.2006.01328.x
 
AbstractObjective: Survival of periodontal ligament cells is crucial issue after tooth replantation. To understand this matter, we introduced MTT assay, which can be used as a tool for measuring the viability of periodontal ligament cells from extracted rat molars. Materials and methods: The maxillary molars of 4-week-old Sprague-Dawley white female rats were used. Ten teeth of each immediate, 1 h Viaspan and 1 h dry were processed for MTT evaluation. Another 10 teeth from each group were treated in the same manner as above, but were replanted into their original socket. After 2 weeks, the animals were killed and the prevalence of resorption pits was evaluated. Results: In vivo MTT assay corresponded with the histological results of the resorption pits (P ≤ 0.05). The polarizing and optical microscopic findings were consistent with the in vivo MTT assay results. Conclusions: In vivo MTT measurements were consistent with the histologic observations and suggest that the in vivo MTT assay could be a tool for evaluating the viability of periodontal ligament cells directly from the extracted root surface. The advantages are shorter analysis time compared with animal or cell culture experiments, easy manipulation, clear quantification and immediate identification of the vital cells. © 2007 The Authors.
 
ISSN1354-523X
2013 Impact Factor: 2.404
 
DOIhttp://dx.doi.org/10.1111/j.1601-0825.2006.01328.x
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorKim, E
 
dc.contributor.authorJeon, IS
 
dc.contributor.authorKim, JW
 
dc.contributor.authorKim, J
 
dc.contributor.authorJung, HS
 
dc.contributor.authorLee, SJ
 
dc.date.accessioned2012-10-25T04:52:44Z
 
dc.date.available2012-10-25T04:52:44Z
 
dc.date.issued2007
 
dc.description.abstractObjective: Survival of periodontal ligament cells is crucial issue after tooth replantation. To understand this matter, we introduced MTT assay, which can be used as a tool for measuring the viability of periodontal ligament cells from extracted rat molars. Materials and methods: The maxillary molars of 4-week-old Sprague-Dawley white female rats were used. Ten teeth of each immediate, 1 h Viaspan and 1 h dry were processed for MTT evaluation. Another 10 teeth from each group were treated in the same manner as above, but were replanted into their original socket. After 2 weeks, the animals were killed and the prevalence of resorption pits was evaluated. Results: In vivo MTT assay corresponded with the histological results of the resorption pits (P ≤ 0.05). The polarizing and optical microscopic findings were consistent with the in vivo MTT assay results. Conclusions: In vivo MTT measurements were consistent with the histologic observations and suggest that the in vivo MTT assay could be a tool for evaluating the viability of periodontal ligament cells directly from the extracted root surface. The advantages are shorter analysis time compared with animal or cell culture experiments, easy manipulation, clear quantification and immediate identification of the vital cells. © 2007 The Authors.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationOral Diseases, 2007, v. 13 n. 5, p. 495-499 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1601-0825.2006.01328.x
 
dc.identifier.citeulike1558252
 
dc.identifier.doihttp://dx.doi.org/10.1111/j.1601-0825.2006.01328.x
 
dc.identifier.epage499
 
dc.identifier.issn1354-523X
2013 Impact Factor: 2.404
 
dc.identifier.issue5
 
dc.identifier.pmid17714353
 
dc.identifier.scopuseid_2-s2.0-34547850940
 
dc.identifier.spage495
 
dc.identifier.urihttp://hdl.handle.net/10722/169545
 
dc.identifier.volume13
 
dc.languageeng
 
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1354-523X&site=1
 
dc.publisher.placeUnited States
 
dc.relation.ispartofOral Diseases
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshCell Survival
 
dc.subject.meshFemale
 
dc.subject.meshFormazans
 
dc.subject.meshIndicators And Reagents
 
dc.subject.meshMolar - Cytology - Pathology
 
dc.subject.meshPeriodontal Ligament - Cytology - Pathology
 
dc.subject.meshRats
 
dc.subject.meshRats, Sprague-Dawley
 
dc.subject.meshRoot Resorption - Etiology - Pathology
 
dc.subject.meshTooth Replantation - Adverse Effects
 
dc.titleAn MTT-based method for quantification of periodontal ligament cell viability
 
dc.typeArticle
 
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Author Affiliations
  1. Yonsei University