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Conference Paper: Association analyses suggest the effects of RANK/RANKL genes on age at menarche in Chinese

TitleAssociation analyses suggest the effects of RANK/RANKL genes on age at menarche in Chinese
Authors
KeywordsMedical sciences
Orthopedics and traumatology medical sciences
Endocrinology
Issue Date2010
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/bone
Citation
The 2010 International Conference on Osteoporosis and Bone Research, Shenzhen, China, 28-31 October 2010. In Bone, v. 47 suppl. 3, p. S374, abstract OP39 How to Cite?
AbstractOBJECTIVE: Age at menarche (AAM), time of first menstrual period, is an important developmental milestone in the female life. In previous studies, RANK (receptor activator of the NF-kB) and RANKL (receptor activator of the NF-kB ligand) were indentified with association of AAM in the Caucasian population. In this study, we conducted association analyses of single nucleotide polymorphisms (SNPs) of RANK and RANKL genes with AAM in Chinese women. METHODS: The total sample consisted of 825women in Changsha, Xi'an and adjacent areas in China. The data of AAM were collected by questionnaire including questions about anthropometric variables, lifestyle, and medical history. Genomic DNA was isolated from leukocytes of peripheral blood using a commercially supplied kit. The SNPs were genotyped using Affymetrix SNP Array 6.0 Set. The association of the SNPs with AAM was analyzed by univariate analysis of variance with Bonferroni correction. The effects of the significant genes on AAM were estimated by linear regression models. The LD patterns of these genes were analyzed using the Haploview program. Then PLINK software was used for analyses of haplotype block association. RESULTS: The mean±SE AAM of the participants was 13.9±0.1 years. In total, 23 SNPs located in or close to RANK and 73 SNPs for RANKL were genotyped. All studied SNPs indicated no deviation from the Hardy–Weinberg equilibrium. Five SNPs in RANK gene showed significant association with AAM: rs7239261 (p=0.006), rs8094884 (p=0.009), rs3826620 (p=0.018), rs8089829 (p=0.034), and rs9956850 (p=0.046). Among these SNPs, rs3826620 was replicated and associated with AAM in our previous study in 306 independent healthy Caucasian females (p=0.022). The contribution of rs7239261, rs8094884, rs3826620, rs8089829, and rs9956850 to the variation ofAAMin Chinesewas 0.5%, 0.7%, 0.2%, 0.3% and 0.1%, respectively. However, no SNPs remained significant after the correction for multiple testing in Chinese. Seven haplotypes of RANK genewere found to be significantly associated with AAM. Three haplotype blocks of RANKL were associated with AAM. An average contribution of each haplotype of RANK and RANKL genes to the variation of AAM in Chinese females was approximately 0.8% and 0.56%, respectively. CONCLUSIONS: This is the first study on RANK and RANKL genes for AAM in Chinese. Our results suggested that RANK and RANKL genes are involved in AAM in different ethnic populations. However, this research warrants the necessity of further more extensive studies.
DescriptionThis journal suppl. is proceedings of the International Conference on Osteoporosis and Bone Research 2010
Persistent Identifierhttp://hdl.handle.net/10722/169453
ISSN
2015 Impact Factor: 3.736
2015 SCImago Journal Rankings: 1.752
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorPan, Ren_US
dc.contributor.authorLiu, YZen_US
dc.contributor.authorDeng, HWen_US
dc.contributor.authorDvornyk, Ven_US
dc.date.accessioned2012-10-18T08:56:18Z-
dc.date.available2012-10-18T08:56:18Z-
dc.date.issued2010en_US
dc.identifier.citationThe 2010 International Conference on Osteoporosis and Bone Research, Shenzhen, China, 28-31 October 2010. In Bone, v. 47 suppl. 3, p. S374, abstract OP39en_US
dc.identifier.issn8756-3282-
dc.identifier.urihttp://hdl.handle.net/10722/169453-
dc.descriptionThis journal suppl. is proceedings of the International Conference on Osteoporosis and Bone Research 2010-
dc.description.abstractOBJECTIVE: Age at menarche (AAM), time of first menstrual period, is an important developmental milestone in the female life. In previous studies, RANK (receptor activator of the NF-kB) and RANKL (receptor activator of the NF-kB ligand) were indentified with association of AAM in the Caucasian population. In this study, we conducted association analyses of single nucleotide polymorphisms (SNPs) of RANK and RANKL genes with AAM in Chinese women. METHODS: The total sample consisted of 825women in Changsha, Xi'an and adjacent areas in China. The data of AAM were collected by questionnaire including questions about anthropometric variables, lifestyle, and medical history. Genomic DNA was isolated from leukocytes of peripheral blood using a commercially supplied kit. The SNPs were genotyped using Affymetrix SNP Array 6.0 Set. The association of the SNPs with AAM was analyzed by univariate analysis of variance with Bonferroni correction. The effects of the significant genes on AAM were estimated by linear regression models. The LD patterns of these genes were analyzed using the Haploview program. Then PLINK software was used for analyses of haplotype block association. RESULTS: The mean±SE AAM of the participants was 13.9±0.1 years. In total, 23 SNPs located in or close to RANK and 73 SNPs for RANKL were genotyped. All studied SNPs indicated no deviation from the Hardy–Weinberg equilibrium. Five SNPs in RANK gene showed significant association with AAM: rs7239261 (p=0.006), rs8094884 (p=0.009), rs3826620 (p=0.018), rs8089829 (p=0.034), and rs9956850 (p=0.046). Among these SNPs, rs3826620 was replicated and associated with AAM in our previous study in 306 independent healthy Caucasian females (p=0.022). The contribution of rs7239261, rs8094884, rs3826620, rs8089829, and rs9956850 to the variation ofAAMin Chinesewas 0.5%, 0.7%, 0.2%, 0.3% and 0.1%, respectively. However, no SNPs remained significant after the correction for multiple testing in Chinese. Seven haplotypes of RANK genewere found to be significantly associated with AAM. Three haplotype blocks of RANKL were associated with AAM. An average contribution of each haplotype of RANK and RANKL genes to the variation of AAM in Chinese females was approximately 0.8% and 0.56%, respectively. CONCLUSIONS: This is the first study on RANK and RANKL genes for AAM in Chinese. Our results suggested that RANK and RANKL genes are involved in AAM in different ethnic populations. However, this research warrants the necessity of further more extensive studies.-
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/bone-
dc.relation.ispartofBoneen_US
dc.subjectMedical sciences-
dc.subjectOrthopedics and traumatology medical sciences-
dc.subjectEndocrinology-
dc.titleAssociation analyses suggest the effects of RANK/RANKL genes on age at menarche in Chineseen_US
dc.typeConference_Paperen_US
dc.identifier.emailDvornyk, V: dvornyk@hku.hken_US
dc.identifier.authorityDvornyk, V=rp00693en_US
dc.identifier.doi10.1016/j.bone.2010.09.128-
dc.identifier.hkuros211991en_US
dc.identifier.volume47-
dc.identifier.issuesuppl. 3-
dc.identifier.spageS374-
dc.identifier.epageS374-
dc.identifier.isiWOS:000285278200067-
dc.publisher.placeUnited States-
dc.description.otherThe 2010 International Conference on Osteoporosis and Bone Research, Shenzhen, China, 28-31 October 2010. In Bone, v. 47 suppl. 3, p. S374, abstract OP39-

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