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Article: The chronic effects of long-term digoxin administration on Na +/K +-ATPase activity in rat tissues

TitleThe chronic effects of long-term digoxin administration on Na +/K +-ATPase activity in rat tissues
Authors
Issue Date1993
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ijcard
Citation
International Journal Of Cardiology, 1993, v. 40 n. 2, p. 95-100 How to Cite?
AbstractWe have studied the effects of digoxin administration on Na +/K +-ATPase activity in heart, liver, muscle, renal medulla and aorta in the rat. Adult male rats were either treated with digoxin for 3 days, 7 days (5 mg/kg per day) or 3 months (3 mg/kg per day). Another group of rats were treated with the vehicle as controls. At the end of the experimental period, blood samples were taken for digoxin measurement, the animals were sacrificed, and the heart, liver, kidney, skeletal muscle and aorta were removed, homogenised and assayed for Na +/K +-ATPase activity. In all tissues except the aorta Na +/K +-ATPase activity was measured by an enzyme coupled reaction. Na +/K +-ATPase activity in the aorta was measured by a fluorometric potassium dependent 3-O-methyl fluorescein phosphatase activity. Plasma digoxin concentration in the digoxin group was 5.34 nmol/l (S.E.M., 0.09) in the 3-day group and 4.38 (0.68) and 4.89 (0.73) nmol/l in the 7-day and 3-month groups, respectively. After treatment for 3 days and 7 days, the Na +/K +ATPase activity in all tissues was significantly lower in the digoxin group (the decrease in activity ranging from 13.4% in muscle to 46.9% in the renal medulla). After 3 months of treatment, Na +/K +-ATPase activity in all the tissues except the aorta was similar in the digoxin and control groups. In the aorta the activity remained low. We conclude that in rats digoxin administration causes upregulation of the Na +/K +ATPase in most tissues.
Persistent Identifierhttp://hdl.handle.net/10722/168911
ISSN
2015 Impact Factor: 4.638
2015 SCImago Journal Rankings: 1.513

 

DC FieldValueLanguage
dc.contributor.authorLi, PWCen_US
dc.contributor.authorHo, CSen_US
dc.contributor.authorSwaminathan, Ren_US
dc.date.accessioned2012-10-08T03:39:35Z-
dc.date.available2012-10-08T03:39:35Z-
dc.date.issued1993en_US
dc.identifier.citationInternational Journal Of Cardiology, 1993, v. 40 n. 2, p. 95-100en_US
dc.identifier.issn0167-5273en_US
dc.identifier.urihttp://hdl.handle.net/10722/168911-
dc.description.abstractWe have studied the effects of digoxin administration on Na +/K +-ATPase activity in heart, liver, muscle, renal medulla and aorta in the rat. Adult male rats were either treated with digoxin for 3 days, 7 days (5 mg/kg per day) or 3 months (3 mg/kg per day). Another group of rats were treated with the vehicle as controls. At the end of the experimental period, blood samples were taken for digoxin measurement, the animals were sacrificed, and the heart, liver, kidney, skeletal muscle and aorta were removed, homogenised and assayed for Na +/K +-ATPase activity. In all tissues except the aorta Na +/K +-ATPase activity was measured by an enzyme coupled reaction. Na +/K +-ATPase activity in the aorta was measured by a fluorometric potassium dependent 3-O-methyl fluorescein phosphatase activity. Plasma digoxin concentration in the digoxin group was 5.34 nmol/l (S.E.M., 0.09) in the 3-day group and 4.38 (0.68) and 4.89 (0.73) nmol/l in the 7-day and 3-month groups, respectively. After treatment for 3 days and 7 days, the Na +/K +ATPase activity in all tissues was significantly lower in the digoxin group (the decrease in activity ranging from 13.4% in muscle to 46.9% in the renal medulla). After 3 months of treatment, Na +/K +-ATPase activity in all the tissues except the aorta was similar in the digoxin and control groups. In the aorta the activity remained low. We conclude that in rats digoxin administration causes upregulation of the Na +/K +ATPase in most tissues.en_US
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ijcarden_US
dc.relation.ispartofInternational Journal of Cardiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAorta - Enzymologyen_US
dc.subject.meshDigoxin - Pharmacologyen_US
dc.subject.meshKidney Medulla - Enzymologyen_US
dc.subject.meshLiver - Enzymologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMuscles - Enzymologyen_US
dc.subject.meshMyocardium - Enzymologyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshSodium-Potassium-Exchanging Atpase - Drug Effects - Metabolismen_US
dc.subject.meshTime Factorsen_US
dc.titleThe chronic effects of long-term digoxin administration on Na +/K +-ATPase activity in rat tissuesen_US
dc.typeArticleen_US
dc.identifier.emailHo, CS:shhoc@hkucc.hku.hken_US
dc.identifier.authorityHo, CS=rp00631en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0167-5273(93)90270-Qen_US
dc.identifier.pmid8394287-
dc.identifier.scopuseid_2-s2.0-0027282270en_US
dc.identifier.volume40en_US
dc.identifier.issue2en_US
dc.identifier.spage95en_US
dc.identifier.epage100en_US
dc.publisher.placeIrelanden_US
dc.identifier.scopusauthoridLi, PWC=34977095900en_US
dc.identifier.scopusauthoridHo, CS=35095289900en_US
dc.identifier.scopusauthoridSwaminathan, R=15038300900en_US

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