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Article: A reporter platform for the Monitoring of in Vivo conformational changes in AcrB

TitleA reporter platform for the Monitoring of in Vivo conformational changes in AcrB
Authors
Issue Date2011
PublisherBentham Science Publishers Ltd. The Journal's web site is located at http://www.bentham.org/ppl/index.htm
Citation
Protein And Peptide Letters, 2011, v. 18 n. 9, p. 863-871 How to Cite?
AbstractAcrB is an inner membrane protein in Escherichia coli that is a component of a triplex AcrA-AcrB-TolC (AcrAB-TolC) multidrug efflux pump. The AcrAB-TolC complex and its homologues are highly conserved among Gramnegative bacteria and are major players in conferring multidrug resistance (MDR) in many pathogens. In this study we developed a disulfide trapping method that may reveal AcrB conformational changes under the native condition in the cell membrane. Specifically, we created seven disulfide bond pairs in the periplasmic domain of AcrB, which can be used as probes to determine local conformational changes. We have developed a rigorous protocol to measure the extent of disulfide bond formation in membrane proteins under the native condition. The rigorousness of the method was verified through examining the extent of disulfide bond formation in Cys pairs separated by different distances. The blockingreducing-labeling scheme combined with fluorescence labeling made the current method convenient, reliable, and quantitative. © 2011 Bentham Science Publishers Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/168535
ISSN
2015 Impact Factor: 1.069
2015 SCImago Journal Rankings: 0.472
References

 

DC FieldValueLanguage
dc.contributor.authorLu, Wen_US
dc.contributor.authorZhong, Men_US
dc.contributor.authorWei, Yen_US
dc.date.accessioned2012-10-08T03:20:13Z-
dc.date.available2012-10-08T03:20:13Z-
dc.date.issued2011en_US
dc.identifier.citationProtein And Peptide Letters, 2011, v. 18 n. 9, p. 863-871en_US
dc.identifier.issn0929-8665en_US
dc.identifier.urihttp://hdl.handle.net/10722/168535-
dc.description.abstractAcrB is an inner membrane protein in Escherichia coli that is a component of a triplex AcrA-AcrB-TolC (AcrAB-TolC) multidrug efflux pump. The AcrAB-TolC complex and its homologues are highly conserved among Gramnegative bacteria and are major players in conferring multidrug resistance (MDR) in many pathogens. In this study we developed a disulfide trapping method that may reveal AcrB conformational changes under the native condition in the cell membrane. Specifically, we created seven disulfide bond pairs in the periplasmic domain of AcrB, which can be used as probes to determine local conformational changes. We have developed a rigorous protocol to measure the extent of disulfide bond formation in membrane proteins under the native condition. The rigorousness of the method was verified through examining the extent of disulfide bond formation in Cys pairs separated by different distances. The blockingreducing-labeling scheme combined with fluorescence labeling made the current method convenient, reliable, and quantitative. © 2011 Bentham Science Publishers Ltd.en_US
dc.languageengen_US
dc.publisherBentham Science Publishers Ltd. The Journal's web site is located at http://www.bentham.org/ppl/index.htmen_US
dc.relation.ispartofProtein and Peptide Lettersen_US
dc.subject.meshCysteine - Analysisen_US
dc.subject.meshDisulfides - Chemistryen_US
dc.subject.meshDrug Resistance, Multiple, Bacterialen_US
dc.subject.meshEscherichia Coli - Chemistryen_US
dc.subject.meshEscherichia Coli Proteins - Chemistryen_US
dc.subject.meshFluorescent Dyes - Analysisen_US
dc.subject.meshModels, Molecularen_US
dc.subject.meshMultidrug Resistance-Associated Proteins - Chemistryen_US
dc.subject.meshProtein Conformationen_US
dc.titleA reporter platform for the Monitoring of in Vivo conformational changes in AcrBen_US
dc.typeArticleen_US
dc.identifier.emailLu, W:luwei@hku.hken_US
dc.identifier.authorityLu, W=rp00754en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.2174/092986611796011446en_US
dc.identifier.pmid21529338-
dc.identifier.scopuseid_2-s2.0-79959419297en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79959419297&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume18en_US
dc.identifier.issue9en_US
dc.identifier.spage863en_US
dc.identifier.epage871en_US
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridLu, W=27868087600en_US
dc.identifier.scopusauthoridZhong, M=36184255700en_US
dc.identifier.scopusauthoridWei, Y=7404094290en_US
dc.identifier.citeulike9430301-

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