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Article: Revisit complexation between DNA and polyethylenimine - Effect of length of free polycationic chains on gene transfection

TitleRevisit complexation between DNA and polyethylenimine - Effect of length of free polycationic chains on gene transfection
Authors
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jconrel
Citation
Journal Of Controlled Release, 2011, v. 152 n. 1, p. 143-151 How to Cite?
Abstract
Our revisit of the complexation between DNA and polyethylenimine (PEI) by using a combination of laser light scattering and gel electrophoresis confirms that nearly all the DNA chains are complexed with PEI to form polyplexes when the molar ratio of nitrogen from PEI to phosphate from DNA (N:P) reaches ~ 3, irrespective of the PEI chain length and solvent. Each solution mixture with N:P > 3 contains two kinds of PEI chains: bound to DNA and free in the solution. It has been shown that it is those free PEI chains that play a vital role in promoting the gene transfection. The effects of the length of the bound and free chains on the gene transfection were respectively studied. Both short and long PEI chains are capable of condensing DNA completely at N:P ~ 3 but long ones are ~ 10 2-fold more effective in the gene transfection, apparently due to their fast endocytosis and intracellular trafficking. The cellular uptake kinetics studied by flow cytometry reveals that long free chains increase the uptake rate constant of the DNA/PEI complexes. In the intracellular pathway, they are able to prevent the development of the later endolysosomes, and facilitate the subsequent release of the polyplexes from the endosomes. Our result shows that the "proton sponge" effect is not dominant because the shut-down of the proton pump only partially attenuates the transfection efficiency. A possible mechanism is speculated and presented. © 2011 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/168532
ISSN
2013 Impact Factor: 7.261
2013 SCImago Journal Rankings: 2.494
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYue, Yen_US
dc.contributor.authorJin, Fen_US
dc.contributor.authorDeng, Ren_US
dc.contributor.authorCai, Jen_US
dc.contributor.authorDai, Zen_US
dc.contributor.authorLin, MCMen_US
dc.contributor.authorKung, HFen_US
dc.contributor.authorMattebjerg, MAen_US
dc.contributor.authorAndresen, TLen_US
dc.contributor.authorWu, Cen_US
dc.date.accessioned2012-10-08T03:20:09Z-
dc.date.available2012-10-08T03:20:09Z-
dc.date.issued2011en_US
dc.identifier.citationJournal Of Controlled Release, 2011, v. 152 n. 1, p. 143-151en_US
dc.identifier.issn0168-3659en_US
dc.identifier.urihttp://hdl.handle.net/10722/168532-
dc.description.abstractOur revisit of the complexation between DNA and polyethylenimine (PEI) by using a combination of laser light scattering and gel electrophoresis confirms that nearly all the DNA chains are complexed with PEI to form polyplexes when the molar ratio of nitrogen from PEI to phosphate from DNA (N:P) reaches ~ 3, irrespective of the PEI chain length and solvent. Each solution mixture with N:P > 3 contains two kinds of PEI chains: bound to DNA and free in the solution. It has been shown that it is those free PEI chains that play a vital role in promoting the gene transfection. The effects of the length of the bound and free chains on the gene transfection were respectively studied. Both short and long PEI chains are capable of condensing DNA completely at N:P ~ 3 but long ones are ~ 10 2-fold more effective in the gene transfection, apparently due to their fast endocytosis and intracellular trafficking. The cellular uptake kinetics studied by flow cytometry reveals that long free chains increase the uptake rate constant of the DNA/PEI complexes. In the intracellular pathway, they are able to prevent the development of the later endolysosomes, and facilitate the subsequent release of the polyplexes from the endosomes. Our result shows that the "proton sponge" effect is not dominant because the shut-down of the proton pump only partially attenuates the transfection efficiency. A possible mechanism is speculated and presented. © 2011 Elsevier B.V.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jconrelen_US
dc.relation.ispartofJournal of Controlled Releaseen_US
dc.subject.meshCell Lineen_US
dc.subject.meshDna - Chemistryen_US
dc.subject.meshEndosomes - Metabolismen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshHumansen_US
dc.subject.meshPolyamines - Chemistryen_US
dc.subject.meshPolyethyleneimine - Chemistryen_US
dc.subject.meshTransfection - Methodsen_US
dc.titleRevisit complexation between DNA and polyethylenimine - Effect of length of free polycationic chains on gene transfectionen_US
dc.typeArticleen_US
dc.identifier.emailLin, MCM:mcllin@hkucc.hku.hken_US
dc.identifier.authorityLin, MCM=rp00746en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jconrel.2011.03.020en_US
dc.identifier.pmid21457737en_US
dc.identifier.scopuseid_2-s2.0-79958118811en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79958118811&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume152en_US
dc.identifier.issue1en_US
dc.identifier.spage143en_US
dc.identifier.epage151en_US
dc.identifier.isiWOS:000292718200018-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridYue, Y=35088987100en_US
dc.identifier.scopusauthoridJin, F=35315971100en_US
dc.identifier.scopusauthoridDeng, R=24467381600en_US
dc.identifier.scopusauthoridCai, J=36668119900en_US
dc.identifier.scopusauthoridDai, Z=36875944200en_US
dc.identifier.scopusauthoridLin, MCM=7404816359en_US
dc.identifier.scopusauthoridKung, HF=7402514190en_US
dc.identifier.scopusauthoridMattebjerg, MA=17135907500en_US
dc.identifier.scopusauthoridAndresen, TL=6603917747en_US
dc.identifier.scopusauthoridWu, C=35075235200en_US
dc.identifier.citeulike9125914-

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