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Article: Revisit complexation between DNA and polyethylenimine - Effect of length of free polycationic chains on gene transfection
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TitleRevisit complexation between DNA and polyethylenimine - Effect of length of free polycationic chains on gene transfection
 
AuthorsYue, Y3
Jin, F3
Deng, R3
Cai, J3
Dai, Z3
Lin, MCM3
Kung, HF3
Mattebjerg, MA1
Andresen, TL1
Wu, C2 3
 
Issue Date2011
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jconrel
 
CitationJournal Of Controlled Release, 2011, v. 152 n. 1, p. 143-151 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jconrel.2011.03.020
 
AbstractOur revisit of the complexation between DNA and polyethylenimine (PEI) by using a combination of laser light scattering and gel electrophoresis confirms that nearly all the DNA chains are complexed with PEI to form polyplexes when the molar ratio of nitrogen from PEI to phosphate from DNA (N:P) reaches ~ 3, irrespective of the PEI chain length and solvent. Each solution mixture with N:P > 3 contains two kinds of PEI chains: bound to DNA and free in the solution. It has been shown that it is those free PEI chains that play a vital role in promoting the gene transfection. The effects of the length of the bound and free chains on the gene transfection were respectively studied. Both short and long PEI chains are capable of condensing DNA completely at N:P ~ 3 but long ones are ~ 10 2-fold more effective in the gene transfection, apparently due to their fast endocytosis and intracellular trafficking. The cellular uptake kinetics studied by flow cytometry reveals that long free chains increase the uptake rate constant of the DNA/PEI complexes. In the intracellular pathway, they are able to prevent the development of the later endolysosomes, and facilitate the subsequent release of the polyplexes from the endosomes. Our result shows that the "proton sponge" effect is not dominant because the shut-down of the proton pump only partially attenuates the transfection efficiency. A possible mechanism is speculated and presented. © 2011 Elsevier B.V.
 
ISSN0168-3659
2012 Impact Factor: 7.633
2012 SCImago Journal Rankings: 2.061
 
DOIhttp://dx.doi.org/10.1016/j.jconrel.2011.03.020
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorYue, Y
 
dc.contributor.authorJin, F
 
dc.contributor.authorDeng, R
 
dc.contributor.authorCai, J
 
dc.contributor.authorDai, Z
 
dc.contributor.authorLin, MCM
 
dc.contributor.authorKung, HF
 
dc.contributor.authorMattebjerg, MA
 
dc.contributor.authorAndresen, TL
 
dc.contributor.authorWu, C
 
dc.date.accessioned2012-10-08T03:20:09Z
 
dc.date.available2012-10-08T03:20:09Z
 
dc.date.issued2011
 
dc.description.abstractOur revisit of the complexation between DNA and polyethylenimine (PEI) by using a combination of laser light scattering and gel electrophoresis confirms that nearly all the DNA chains are complexed with PEI to form polyplexes when the molar ratio of nitrogen from PEI to phosphate from DNA (N:P) reaches ~ 3, irrespective of the PEI chain length and solvent. Each solution mixture with N:P > 3 contains two kinds of PEI chains: bound to DNA and free in the solution. It has been shown that it is those free PEI chains that play a vital role in promoting the gene transfection. The effects of the length of the bound and free chains on the gene transfection were respectively studied. Both short and long PEI chains are capable of condensing DNA completely at N:P ~ 3 but long ones are ~ 10 2-fold more effective in the gene transfection, apparently due to their fast endocytosis and intracellular trafficking. The cellular uptake kinetics studied by flow cytometry reveals that long free chains increase the uptake rate constant of the DNA/PEI complexes. In the intracellular pathway, they are able to prevent the development of the later endolysosomes, and facilitate the subsequent release of the polyplexes from the endosomes. Our result shows that the "proton sponge" effect is not dominant because the shut-down of the proton pump only partially attenuates the transfection efficiency. A possible mechanism is speculated and presented. © 2011 Elsevier B.V.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Controlled Release, 2011, v. 152 n. 1, p. 143-151 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jconrel.2011.03.020
 
dc.identifier.citeulike9125914
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.jconrel.2011.03.020
 
dc.identifier.epage151
 
dc.identifier.issn0168-3659
2012 Impact Factor: 7.633
2012 SCImago Journal Rankings: 2.061
 
dc.identifier.issue1
 
dc.identifier.pmid21457737
 
dc.identifier.scopuseid_2-s2.0-79958118811
 
dc.identifier.spage143
 
dc.identifier.urihttp://hdl.handle.net/10722/168532
 
dc.identifier.volume152
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jconrel
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofJournal of Controlled Release
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshCell Line
 
dc.subject.meshDna - Chemistry
 
dc.subject.meshEndosomes - Metabolism
 
dc.subject.meshFlow Cytometry
 
dc.subject.meshHumans
 
dc.subject.meshPolyamines - Chemistry
 
dc.subject.meshPolyethyleneimine - Chemistry
 
dc.subject.meshTransfection - Methods
 
dc.titleRevisit complexation between DNA and polyethylenimine - Effect of length of free polycationic chains on gene transfection
 
dc.typeArticle
 
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<contributor.author>Lin, MCM</contributor.author>
<contributor.author>Kung, HF</contributor.author>
<contributor.author>Mattebjerg, MA</contributor.author>
<contributor.author>Andresen, TL</contributor.author>
<contributor.author>Wu, C</contributor.author>
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<description.abstract>Our revisit of the complexation between DNA and polyethylenimine (PEI) by using a combination of laser light scattering and gel electrophoresis confirms that nearly all the DNA chains are complexed with PEI to form polyplexes when the molar ratio of nitrogen from PEI to phosphate from DNA (N:P) reaches ~ 3, irrespective of the PEI chain length and solvent. Each solution mixture with N:P &gt; 3 contains two kinds of PEI chains: bound to DNA and free in the solution. It has been shown that it is those free PEI chains that play a vital role in promoting the gene transfection. The effects of the length of the bound and free chains on the gene transfection were respectively studied. Both short and long PEI chains are capable of condensing DNA completely at N:P ~ 3 but long ones are ~ 10 2-fold more effective in the gene transfection, apparently due to their fast endocytosis and intracellular trafficking. The cellular uptake kinetics studied by flow cytometry reveals that long free chains increase the uptake rate constant of the DNA/PEI complexes. In the intracellular pathway, they are able to prevent the development of the later endolysosomes, and facilitate the subsequent release of the polyplexes from the endosomes. Our result shows that the &quot;proton sponge&quot; effect is not dominant because the shut-down of the proton pump only partially attenuates the transfection efficiency. A possible mechanism is speculated and presented. &#169; 2011 Elsevier B.V.</description.abstract>
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Author Affiliations
  1. Danmarks Tekniske Universitet
  2. University of Science and Technology of China
  3. Chinese University of Hong Kong