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Article: Proteomic identification of microRNA-122a target proteins in hepatocellular carcinoma

TitleProteomic identification of microRNA-122a target proteins in hepatocellular carcinoma
Authors
Issue Date2010
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomics
Citation
Proteomics, 2010, v. 10 n. 20, p. 3723-3731 How to Cite?
AbstractmicroRNA-122a (miR-122a) is a liver-specific miRNA that is frequently downregulated in hepatocellular carcinoma (HCC). The exact functional role of miR-122a and its target in HCC remain largely unknown. We developed a lentiviral vector for the expression of pre-miR-122a (Lenti-miR-122a). Lenti-miR-122a inhibited HCC cell growth and induced apoptosis in vitro. We employed proteomic profiling to identify the target proteins of miR-122a. In total, ten proteins with differential expression in HCC cells infected with Lenti-miR-122a were identified. Amongst them, downregulation of peroxiredoxin 2 (PRDXII) by miR-122a was validated by Western blotting. Using bioinformatics analysis, predictable target sites of miR-122a were identified in the 5′-UTR of PRDXII mRNA. Luciferase reporter assay confirmed the regulation of miR-122a on 5′-UTR of PRDXII. In conclusion, PRDXII was identified to be the new target of miR-122a. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Persistent Identifierhttp://hdl.handle.net/10722/168491
ISSN
2015 Impact Factor: 4.079
2015 SCImago Journal Rankings: 1.476
References

 

DC FieldValueLanguage
dc.contributor.authorDiao, Sen_US
dc.contributor.authorZhang, JFen_US
dc.contributor.authorWang, Hen_US
dc.contributor.authorHe, MLen_US
dc.contributor.authorLin, MCMen_US
dc.contributor.authorChen, Yen_US
dc.contributor.authorKung, HFen_US
dc.date.accessioned2012-10-08T03:19:35Z-
dc.date.available2012-10-08T03:19:35Z-
dc.date.issued2010en_US
dc.identifier.citationProteomics, 2010, v. 10 n. 20, p. 3723-3731en_US
dc.identifier.issn1615-9853en_US
dc.identifier.urihttp://hdl.handle.net/10722/168491-
dc.description.abstractmicroRNA-122a (miR-122a) is a liver-specific miRNA that is frequently downregulated in hepatocellular carcinoma (HCC). The exact functional role of miR-122a and its target in HCC remain largely unknown. We developed a lentiviral vector for the expression of pre-miR-122a (Lenti-miR-122a). Lenti-miR-122a inhibited HCC cell growth and induced apoptosis in vitro. We employed proteomic profiling to identify the target proteins of miR-122a. In total, ten proteins with differential expression in HCC cells infected with Lenti-miR-122a were identified. Amongst them, downregulation of peroxiredoxin 2 (PRDXII) by miR-122a was validated by Western blotting. Using bioinformatics analysis, predictable target sites of miR-122a were identified in the 5′-UTR of PRDXII mRNA. Luciferase reporter assay confirmed the regulation of miR-122a on 5′-UTR of PRDXII. In conclusion, PRDXII was identified to be the new target of miR-122a. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.en_US
dc.languageengen_US
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomicsen_US
dc.relation.ispartofProteomicsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosis - Geneticsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCarcinoma, Hepatocellular - Genetics - Metabolism - Pathologyen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshGene Expression Regulation, Neoplasticen_US
dc.subject.meshHumansen_US
dc.subject.meshLiver Neoplasms - Genetics - Metabolism - Pathologyen_US
dc.subject.meshMicrornas - Genetics - Metabolismen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNeoplasm Proteins - Genetics - Metabolismen_US
dc.subject.meshPeroxiredoxins - Genetics - Metabolismen_US
dc.subject.meshProteome - Analysisen_US
dc.subject.meshSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionizationen_US
dc.titleProteomic identification of microRNA-122a target proteins in hepatocellular carcinomaen_US
dc.typeArticleen_US
dc.identifier.emailLin, MCM:mcllin@hkucc.hku.hken_US
dc.identifier.authorityLin, MCM=rp00746en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/pmic.201000050en_US
dc.identifier.pmid20859956-
dc.identifier.scopuseid_2-s2.0-78649403064en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78649403064&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume10en_US
dc.identifier.issue20en_US
dc.identifier.spage3723en_US
dc.identifier.epage3731en_US
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridDiao, S=8939037300en_US
dc.identifier.scopusauthoridZhang, JF=13007942600en_US
dc.identifier.scopusauthoridWang, H=7501747965en_US
dc.identifier.scopusauthoridHe, ML=35080389700en_US
dc.identifier.scopusauthoridLin, MCM=7404816359en_US
dc.identifier.scopusauthoridChen, Y=24075600300en_US
dc.identifier.scopusauthoridKung, HF=7402514190en_US

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