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Article: Capillary electrophoresis with immobilized quantum dot fluorescence detection for rapid determination of organophosphorus pesticides in vegetables

TitleCapillary electrophoresis with immobilized quantum dot fluorescence detection for rapid determination of organophosphorus pesticides in vegetables
Authors
Issue Date2010
Citation
Electrophoresis, 2010, v. 31 n. 18, p. 3107-3114 How to Cite?
AbstractBased on the highly sensitive and selective fluorescence enhancement of water-soluble CdTe/CdS core-shell quantum dots (QD) by organophosphorus pesticides (OPs such as mevinphos, phosalone, methidathion and diazinon), a simple, rapid and selective method is developed using CE with QD/LIF detection (473 nm excitation/532 nm fluorescence) to determine OPs in vegetable samples. The method enables the use of a simple pretreatment procedure based only on solvent extraction and eliminates the use of a time-consuming SPE step. A novel procedure is developed to immobilize QD onto the inside capillary surface via the formation of a silane coupling mercaptopropyl-trimethoxysilane network. Under optimized CE conditions, baseline separation for all four OPs was observed within 12 min. The CE-QD/LIF method was shown to have a detection limit from 50 to 180 μg/kg, working ranges 0.1-30 mg/kg, recoveries 88.7-96.1% and repeatability (RSD, n = 3) 0.36-0.75% for migration time and 2.9-5.7% for peak height. For tomato samples, the detection limits were more than ten times lower than maximum residue levels specified by the Codex Alimentarius Commission for all four OPs investigated. The method thus satisfies the need for a simple, quick and selective method to determine residual OPs in complex vegetable matrix as required by the increasingly strict regulations for health protection introduced in recent years. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
Persistent Identifierhttp://hdl.handle.net/10722/168476
ISSN
2015 Impact Factor: 2.482
2015 SCImago Journal Rankings: 0.896
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Qen_US
dc.contributor.authorFung, Yen_US
dc.date.accessioned2012-10-08T03:19:25Z-
dc.date.available2012-10-08T03:19:25Z-
dc.date.issued2010en_US
dc.identifier.citationElectrophoresis, 2010, v. 31 n. 18, p. 3107-3114en_US
dc.identifier.issn0173-0835en_US
dc.identifier.urihttp://hdl.handle.net/10722/168476-
dc.description.abstractBased on the highly sensitive and selective fluorescence enhancement of water-soluble CdTe/CdS core-shell quantum dots (QD) by organophosphorus pesticides (OPs such as mevinphos, phosalone, methidathion and diazinon), a simple, rapid and selective method is developed using CE with QD/LIF detection (473 nm excitation/532 nm fluorescence) to determine OPs in vegetable samples. The method enables the use of a simple pretreatment procedure based only on solvent extraction and eliminates the use of a time-consuming SPE step. A novel procedure is developed to immobilize QD onto the inside capillary surface via the formation of a silane coupling mercaptopropyl-trimethoxysilane network. Under optimized CE conditions, baseline separation for all four OPs was observed within 12 min. The CE-QD/LIF method was shown to have a detection limit from 50 to 180 μg/kg, working ranges 0.1-30 mg/kg, recoveries 88.7-96.1% and repeatability (RSD, n = 3) 0.36-0.75% for migration time and 2.9-5.7% for peak height. For tomato samples, the detection limits were more than ten times lower than maximum residue levels specified by the Codex Alimentarius Commission for all four OPs investigated. The method thus satisfies the need for a simple, quick and selective method to determine residual OPs in complex vegetable matrix as required by the increasingly strict regulations for health protection introduced in recent years. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.en_US
dc.languageengen_US
dc.relation.ispartofElectrophoresisen_US
dc.subject.meshChromatography, Micellar Electrokinetic Capillary - Methodsen_US
dc.subject.meshElectrophoresis, Capillary - Instrumentation - Methodsen_US
dc.subject.meshEquipment Designen_US
dc.subject.meshFood Contaminationen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshLinear Modelsen_US
dc.subject.meshMicroscopy, Electron, Scanningen_US
dc.subject.meshOrganophosphorus Compounds - Analysisen_US
dc.subject.meshPesticide Residues - Analysisen_US
dc.subject.meshQuantum Dotsen_US
dc.subject.meshReproducibility Of Resultsen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshSpectrometry, Fluorescence - Methodsen_US
dc.subject.meshVegetables - Chemistryen_US
dc.titleCapillary electrophoresis with immobilized quantum dot fluorescence detection for rapid determination of organophosphorus pesticides in vegetablesen_US
dc.typeArticleen_US
dc.identifier.emailFung, Y:ysfung@hku.hken_US
dc.identifier.authorityFung, Y=rp00697en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/elps.201000260en_US
dc.identifier.pmid20872612-
dc.identifier.scopuseid_2-s2.0-77956937304en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77956937304&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume31en_US
dc.identifier.issue18en_US
dc.identifier.spage3107en_US
dc.identifier.epage3114en_US
dc.identifier.isiWOS:000283389900013-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridChen, Q=8060037500en_US
dc.identifier.scopusauthoridFung, Y=13309754700en_US

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