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Article: Butyrate mediates nucleotide-binding and oligomerisation domain (NOD) 2-dependent mucosal immune responses against peptidoglycan

TitleButyrate mediates nucleotide-binding and oligomerisation domain (NOD) 2-dependent mucosal immune responses against peptidoglycan
Authors
Issue Date2009
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.eji.de
Citation
European Journal Of Immunology, 2009, v. 39 n. 12, p. 3529-3537 How to Cite?
AbstractThe interaction between digestive tract microbiological flora and food has an important influence on human health. Butyrate is produced during the fermentation of dietary fibres by intestinal bacteria and plays an important role in the regulation of mucosal immunity. In this report, we studied the impact of butyrate on the defence mechanism against the bacterial membrane component peptidoglycan (PGN). Butyrate was found to enhance PGN-mediated IL-8 and GRO-α production. The expression of these chemokines required the activation of NF-κB and was dependent on the concentrations of butyrate and PGN. Butyrate was found to up-regulate nucleotide-binding and oligomerisation domain (NOD) 2, but not NOD1 or TLR2. NOD2 up-regulation was mediated by an increase in histone acetylation in the Nod2 promoter region, leading to enhanced PGN-induced IL-8 and GRO-α secretion. Knockdown of NOD2 and TLR2 by siRNA significantly reduced PGN-mediated chemokine production, suggesting that both NOD2 and TLR2 are required for maximal response. Our findings provide a better understanding of the mechanism by which butyrate regulates mucosal immunity for normal intestinal function. Based on the results of this study, we infer that dietary fibres can impact inflammatory bowel diseases. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
Persistent Identifierhttp://hdl.handle.net/10722/168424
ISSN
2015 Impact Factor: 4.179
2015 SCImago Journal Rankings: 2.568
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, CHen_US
dc.contributor.authorLam, Wen_US
dc.contributor.authorMa, DLen_US
dc.contributor.authorGullen, EAen_US
dc.contributor.authorCheng, YCen_US
dc.date.accessioned2012-10-08T03:18:47Z-
dc.date.available2012-10-08T03:18:47Z-
dc.date.issued2009en_US
dc.identifier.citationEuropean Journal Of Immunology, 2009, v. 39 n. 12, p. 3529-3537en_US
dc.identifier.issn0014-2980en_US
dc.identifier.urihttp://hdl.handle.net/10722/168424-
dc.description.abstractThe interaction between digestive tract microbiological flora and food has an important influence on human health. Butyrate is produced during the fermentation of dietary fibres by intestinal bacteria and plays an important role in the regulation of mucosal immunity. In this report, we studied the impact of butyrate on the defence mechanism against the bacterial membrane component peptidoglycan (PGN). Butyrate was found to enhance PGN-mediated IL-8 and GRO-α production. The expression of these chemokines required the activation of NF-κB and was dependent on the concentrations of butyrate and PGN. Butyrate was found to up-regulate nucleotide-binding and oligomerisation domain (NOD) 2, but not NOD1 or TLR2. NOD2 up-regulation was mediated by an increase in histone acetylation in the Nod2 promoter region, leading to enhanced PGN-induced IL-8 and GRO-α secretion. Knockdown of NOD2 and TLR2 by siRNA significantly reduced PGN-mediated chemokine production, suggesting that both NOD2 and TLR2 are required for maximal response. Our findings provide a better understanding of the mechanism by which butyrate regulates mucosal immunity for normal intestinal function. Based on the results of this study, we infer that dietary fibres can impact inflammatory bowel diseases. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.en_US
dc.languageengen_US
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.eji.deen_US
dc.relation.ispartofEuropean Journal of Immunologyen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshButyrates - Pharmacologyen_US
dc.subject.meshCaco-2 Cellsen_US
dc.subject.meshChemokine Cxcl1 - Genetics - Metabolismen_US
dc.subject.meshChemokines - Metabolism - Secretionen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunity, Mucosal - Drug Effectsen_US
dc.subject.meshInterleukin-8 - Genetics - Metabolismen_US
dc.subject.meshNf-Kappa B - Genetics - Metabolismen_US
dc.subject.meshNod1 Signaling Adaptor Protein - Genetics - Metabolismen_US
dc.subject.meshNod2 Signaling Adaptor Protein - Genetics - Metabolismen_US
dc.subject.meshPeptidoglycan - Pharmacologyen_US
dc.subject.meshRna Interferenceen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshToll-Like Receptor 2 - Genetics - Metabolismen_US
dc.subject.meshUp-Regulation - Drug Effectsen_US
dc.titleButyrate mediates nucleotide-binding and oligomerisation domain (NOD) 2-dependent mucosal immune responses against peptidoglycanen_US
dc.typeArticleen_US
dc.identifier.emailLeung, CH:duncanl@hkucc.hku.hken_US
dc.identifier.emailMa, DL:edmondma@hku.hken_US
dc.identifier.authorityLeung, CH=rp00730en_US
dc.identifier.authorityMa, DL=rp00760en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1002/eji.200939454en_US
dc.identifier.pmid19830732-
dc.identifier.scopuseid_2-s2.0-73249125168en_US
dc.identifier.hkuros169753-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-73249125168&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume39en_US
dc.identifier.issue12en_US
dc.identifier.spage3529en_US
dc.identifier.epage3537en_US
dc.identifier.isiWOS:000272928300029-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridLeung, CH=7402612570en_US
dc.identifier.scopusauthoridLam, W=7203021943en_US
dc.identifier.scopusauthoridMa, DL=7402075538en_US
dc.identifier.scopusauthoridGullen, EA=6602138704en_US
dc.identifier.scopusauthoridCheng, YC=36041844200en_US

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