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Article: The effect of nitric oxide on metal release from metallothionein-3: Gradual unfolding of the protein

TitleThe effect of nitric oxide on metal release from metallothionein-3: Gradual unfolding of the protein
Authors
Issue Date2008
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00775/index.htm
Citation
Journal Of Biological Inorganic Chemistry, 2008, v. 13 n. 3, p. 411-419 How to Cite?
AbstractMetallothionein-3 (MT-3), or neuronal growth inhibitory factor, which exhibits growth inhibitory activity, is a brain-specific metallothionein. In this study, the effect of nitric oxide (NO) on metal release (using Cd 2+ as a probe) from MT-3 was examined by 113Cd and 2D [1H-15N] heteronuclear single-quantum coherence NMR spectroscopy. The exposure of human MT-3 to NO leads to a nonselective release of the three metals from the β-domain. In contrast to metallothionein-1 and metallothionein-2, two of the bound metals in the α-domain were also partially released, with the domain structure remaining almost unchanged. Further addition of NO resulted in the complete release of metals and concomitant unfolding of the protein. The preference of release of the two metals in the α-domain was attributed to the presence of two slightly different coordination environments for the four cadmium/zinc atoms. © 2007 SBIC.
Persistent Identifierhttp://hdl.handle.net/10722/168285
ISSN
2015 Impact Factor: 2.495
2015 SCImago Journal Rankings: 0.882
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Hen_US
dc.contributor.authorLi, Hen_US
dc.contributor.authorCai, Ben_US
dc.contributor.authorHuang, ZXen_US
dc.contributor.authorSun, Hen_US
dc.date.accessioned2012-10-08T03:17:03Z-
dc.date.available2012-10-08T03:17:03Z-
dc.date.issued2008en_US
dc.identifier.citationJournal Of Biological Inorganic Chemistry, 2008, v. 13 n. 3, p. 411-419en_US
dc.identifier.issn0949-8257en_US
dc.identifier.urihttp://hdl.handle.net/10722/168285-
dc.description.abstractMetallothionein-3 (MT-3), or neuronal growth inhibitory factor, which exhibits growth inhibitory activity, is a brain-specific metallothionein. In this study, the effect of nitric oxide (NO) on metal release (using Cd 2+ as a probe) from MT-3 was examined by 113Cd and 2D [1H-15N] heteronuclear single-quantum coherence NMR spectroscopy. The exposure of human MT-3 to NO leads to a nonselective release of the three metals from the β-domain. In contrast to metallothionein-1 and metallothionein-2, two of the bound metals in the α-domain were also partially released, with the domain structure remaining almost unchanged. Further addition of NO resulted in the complete release of metals and concomitant unfolding of the protein. The preference of release of the two metals in the α-domain was attributed to the presence of two slightly different coordination environments for the four cadmium/zinc atoms. © 2007 SBIC.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00775/index.htmen_US
dc.relation.ispartofJournal of Biological Inorganic Chemistryen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshHumansen_US
dc.subject.meshMetallothionein - Chemistryen_US
dc.subject.meshMetals - Chemistryen_US
dc.subject.meshModels, Molecularen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNitric Oxide - Chemistryen_US
dc.subject.meshNuclear Magnetic Resonance, Biomolecularen_US
dc.subject.meshProtein Denaturationen_US
dc.subject.meshSequence Homology, Amino Aciden_US
dc.titleThe effect of nitric oxide on metal release from metallothionein-3: Gradual unfolding of the proteinen_US
dc.typeArticleen_US
dc.identifier.emailSun, H:hsun@hkucc.hku.hken_US
dc.identifier.authoritySun, H=rp00777en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00775-007-0331-xen_US
dc.identifier.pmid18157556-
dc.identifier.scopuseid_2-s2.0-40949135318en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-40949135318&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume13en_US
dc.identifier.issue3en_US
dc.identifier.spage411en_US
dc.identifier.epage419en_US
dc.identifier.isiWOS:000253994300009-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridWang, H=15052706700en_US
dc.identifier.scopusauthoridLi, H=14023043100en_US
dc.identifier.scopusauthoridCai, B=36484162900en_US
dc.identifier.scopusauthoridHuang, ZX=7406221847en_US
dc.identifier.scopusauthoridSun, H=7404827446en_US
dc.identifier.citeulike2752403-

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