File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1073/pnas.0609883104
- Scopus: eid_2-s2.0-34347223650
- PMID: 17488817
- WOS: WOS:000246599900060
- Find via
Supplementary
-
Bookmarks:
- CiteULike: 1
- Citations:
- Appears in Collections:
Article: Unique antiviral mechanism discovered in anti-hepatitis B virus research with a natural product analogue
Title | Unique antiviral mechanism discovered in anti-hepatitis B virus research with a natural product analogue |
---|---|
Authors | |
Keywords | HBV promoters Helioxanthin Hepatocyte nuclear factors |
Issue Date | 2007 |
Publisher | National Academy of Sciences. The Journal's web site is located at http://www.pnas.org |
Citation | Proceedings Of The National Academy Of Sciences Of The United States Of America, 2007, v. 104 n. 20, p. 8526-8531 How to Cite? |
Abstract | Helioxanthin is a natural product that inhibits the replication of a number of viruses. We found that a previously undescribed helioxanthin analogue, 8-1, exhibited potent anti-hepatitis B virus (HBV) activity with little cytotoxicity. 8-1 suppressed both HBV RNA and protein expression, as well as DNA replication of both wildtype and 3TC-resistant virus. Time-course analyses revealed that RNA expression was blocked first after treatment with 8-1, followed by viral proteins, and then DNA. 8-1 inhibited the activity of all HBV promoters by decreasing the binding of hepatocyte nuclear factor 4 (HNF-4), HNF-3, and fetoprotein factor to the precore/core promoter enhancer II region. The amount of HNF-4 and HNF-3 was decreased posttranscriptionally by 8-1 in HBV-producing cells, but not in HBV-negative cells. Therefore, 8-1 suppresses HBV replication by posttranscriptional down-regulation of critical transcription factors in HBV-producing cells, thus diminishing HBV promoter activity and blocking viral gene expression and replication. This mechanism is unique and different from other anti-HBV compounds previously described. © 2007 by The National Academy of Sciences of the USA. |
Persistent Identifier | http://hdl.handle.net/10722/168124 |
ISSN | 2023 Impact Factor: 9.4 2023 SCImago Journal Rankings: 3.737 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ying, C | en_US |
dc.contributor.author | Li, Y | en_US |
dc.contributor.author | Leung, CH | en_US |
dc.contributor.author | Robek, MD | en_US |
dc.contributor.author | Cheng, YC | en_US |
dc.date.accessioned | 2012-10-08T03:15:21Z | - |
dc.date.available | 2012-10-08T03:15:21Z | - |
dc.date.issued | 2007 | en_US |
dc.identifier.citation | Proceedings Of The National Academy Of Sciences Of The United States Of America, 2007, v. 104 n. 20, p. 8526-8531 | en_US |
dc.identifier.issn | 0027-8424 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/168124 | - |
dc.description.abstract | Helioxanthin is a natural product that inhibits the replication of a number of viruses. We found that a previously undescribed helioxanthin analogue, 8-1, exhibited potent anti-hepatitis B virus (HBV) activity with little cytotoxicity. 8-1 suppressed both HBV RNA and protein expression, as well as DNA replication of both wildtype and 3TC-resistant virus. Time-course analyses revealed that RNA expression was blocked first after treatment with 8-1, followed by viral proteins, and then DNA. 8-1 inhibited the activity of all HBV promoters by decreasing the binding of hepatocyte nuclear factor 4 (HNF-4), HNF-3, and fetoprotein factor to the precore/core promoter enhancer II region. The amount of HNF-4 and HNF-3 was decreased posttranscriptionally by 8-1 in HBV-producing cells, but not in HBV-negative cells. Therefore, 8-1 suppresses HBV replication by posttranscriptional down-regulation of critical transcription factors in HBV-producing cells, thus diminishing HBV promoter activity and blocking viral gene expression and replication. This mechanism is unique and different from other anti-HBV compounds previously described. © 2007 by The National Academy of Sciences of the USA. | en_US |
dc.language | eng | en_US |
dc.publisher | National Academy of Sciences. The Journal's web site is located at http://www.pnas.org | en_US |
dc.relation.ispartof | Proceedings of the National Academy of Sciences of the United States of America | en_US |
dc.subject | HBV promoters | - |
dc.subject | Helioxanthin | - |
dc.subject | Hepatocyte nuclear factors | - |
dc.subject.mesh | Antiviral Agents - Chemistry - Pharmacology | en_US |
dc.subject.mesh | Benzodioxoles - Chemistry - Pharmacology | en_US |
dc.subject.mesh | Cell Death - Drug Effects | en_US |
dc.subject.mesh | Cell Line | en_US |
dc.subject.mesh | Dna, Viral - Metabolism | en_US |
dc.subject.mesh | Down-Regulation - Drug Effects | en_US |
dc.subject.mesh | Hepatitis B Virus - Drug Effects - Genetics | en_US |
dc.subject.mesh | Hepatocyte Nuclear Factors - Genetics - Metabolism | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Lamivudine - Pharmacology | en_US |
dc.subject.mesh | Lignans | en_US |
dc.subject.mesh | Models, Biological | en_US |
dc.subject.mesh | Phthalazines - Chemistry - Pharmacology | en_US |
dc.subject.mesh | Promoter Regions, Genetic - Genetics | en_US |
dc.subject.mesh | Protein Binding - Drug Effects | en_US |
dc.subject.mesh | Rna Stability - Drug Effects | en_US |
dc.subject.mesh | Rna, Viral - Metabolism | en_US |
dc.subject.mesh | Thermodynamics | en_US |
dc.subject.mesh | Time Factors | en_US |
dc.subject.mesh | Transcription, Genetic - Drug Effects | en_US |
dc.subject.mesh | Viral Proteins - Metabolism | en_US |
dc.subject.mesh | Xanthines - Chemistry - Pharmacology | en_US |
dc.title | Unique antiviral mechanism discovered in anti-hepatitis B virus research with a natural product analogue | en_US |
dc.type | Article | en_US |
dc.identifier.email | Leung, CH:duncanl@hkucc.hku.hk | en_US |
dc.identifier.authority | Leung, CH=rp00730 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1073/pnas.0609883104 | en_US |
dc.identifier.pmid | 17488817 | - |
dc.identifier.scopus | eid_2-s2.0-34347223650 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-34347223650&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 104 | en_US |
dc.identifier.issue | 20 | en_US |
dc.identifier.spage | 8526 | en_US |
dc.identifier.epage | 8531 | en_US |
dc.identifier.isi | WOS:000246599900060 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Ying, C=7101886519 | en_US |
dc.identifier.scopusauthorid | Li, Y=26642866500 | en_US |
dc.identifier.scopusauthorid | Leung, CH=7402612570 | en_US |
dc.identifier.scopusauthorid | Robek, MD=6507620977 | en_US |
dc.identifier.scopusauthorid | Cheng, YC=36041844200 | en_US |
dc.identifier.citeulike | 2192598 | - |
dc.identifier.issnl | 0027-8424 | - |