File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: 1H NMR studies of the imidazole complex of cytochrome c: Resonance assignment and structural characterization of the heme cavity

Title1H NMR studies of the imidazole complex of cytochrome c: Resonance assignment and structural characterization of the heme cavity
Authors
Issue Date1995
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/ic
Citation
Inorganic Chemistry, 1995, v. 34 n. 3, p. 680-687 How to Cite?
AbstractTwo-dimensional 1H nuclear magnetic resonance exchange correlated spectroscopy (2D EXSY), 1D saturation transfer, and nuclear Overhauser effect (NOE) experiments have been utilized in conjunction with isotope labeling to allow a comprehensive assignment of the hyperfine shifted resonances in the low spin imidazole complex of cytochrome c (Im-cyt c). Various two-dimensional phase-sensitive experiments (EXSY, DQF-COSY, TOCSY) are combined to assign the majority of the heme pocket side-chain proton signals. 1D NOE and 2D NOESY spectra of Im-cyt c are presented. Qualitative interpretation of NOE data indicates the structure in the heme pocket of Im-cyt c has changed relative to cyt c. Large conformational changes are apparent on the methionine side of the heme in the environment of the axial ligand. On the basis of the NOEs observed, the nearly parallel orientation of the bound imidazole to the plane of His18 imidazole has also been obtained, which agrees with the characteristic EPR g values similar to that of cytochrome b5. © 1995 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/168068
ISSN
2015 Impact Factor: 4.82
2015 SCImago Journal Rankings: 1.873
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorShao, Wen_US
dc.contributor.authorSun, Hen_US
dc.contributor.authorYao, Yen_US
dc.contributor.authorTang, Wen_US
dc.date.accessioned2012-10-08T03:14:44Z-
dc.date.available2012-10-08T03:14:44Z-
dc.date.issued1995en_US
dc.identifier.citationInorganic Chemistry, 1995, v. 34 n. 3, p. 680-687en_US
dc.identifier.issn0020-1669en_US
dc.identifier.urihttp://hdl.handle.net/10722/168068-
dc.description.abstractTwo-dimensional 1H nuclear magnetic resonance exchange correlated spectroscopy (2D EXSY), 1D saturation transfer, and nuclear Overhauser effect (NOE) experiments have been utilized in conjunction with isotope labeling to allow a comprehensive assignment of the hyperfine shifted resonances in the low spin imidazole complex of cytochrome c (Im-cyt c). Various two-dimensional phase-sensitive experiments (EXSY, DQF-COSY, TOCSY) are combined to assign the majority of the heme pocket side-chain proton signals. 1D NOE and 2D NOESY spectra of Im-cyt c are presented. Qualitative interpretation of NOE data indicates the structure in the heme pocket of Im-cyt c has changed relative to cyt c. Large conformational changes are apparent on the methionine side of the heme in the environment of the axial ligand. On the basis of the NOEs observed, the nearly parallel orientation of the bound imidazole to the plane of His18 imidazole has also been obtained, which agrees with the characteristic EPR g values similar to that of cytochrome b5. © 1995 American Chemical Society.en_US
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/icen_US
dc.relation.ispartofInorganic Chemistryen_US
dc.title1H NMR studies of the imidazole complex of cytochrome c: Resonance assignment and structural characterization of the heme cavityen_US
dc.typeArticleen_US
dc.identifier.emailSun, H:hsun@hkucc.hku.hken_US
dc.identifier.authoritySun, H=rp00777en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/ic00107a023-
dc.identifier.scopuseid_2-s2.0-33751154791en_US
dc.identifier.volume34en_US
dc.identifier.issue3en_US
dc.identifier.spage680en_US
dc.identifier.epage687en_US
dc.identifier.isiWOS:A1995QE84000023-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridShao, W=7102687650en_US
dc.identifier.scopusauthoridSun, H=7404827446en_US
dc.identifier.scopusauthoridYao, Y=10142468300en_US
dc.identifier.scopusauthoridTang, W=7403430524en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats