File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Flow analysis coupled with PQC/DNA biosensor for assay of E. coli based on detecting DNA products from PCR amplification

TitleFlow analysis coupled with PQC/DNA biosensor for assay of E. coli based on detecting DNA products from PCR amplification
Authors
Issue Date2006
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bios
Citation
Biosensors And Bioelectronics, 2006, v. 22 n. 4 SPEC. ISS., p. 506-512 How to Cite?
AbstractA flow-through PQC/DNA biosensor system is developed by combining sequential flow polymerase chain reaction (PCR) products denaturing prior to piezoelectric quartz crystal (PQC) detection via hybridization of ssDNA. The PQC/DNA biosensor is fabricated based on complex formation of neutravidin/biotinylated probe in 0.2 M NaCl in TE buffer (10 mM Tris, 1 mM EDTA, pH 7.5). Results show that the coating fabricated provides a desirable quality with satisfactory performance. Its application for Escherichia coli detection under controlled flow at 0.02 mL/min for denaturing PCR products and 10 mL/min for transferring solution between reactors and delivering samples to detector to reduce rehybridization leads to significant improvement in repeatability (R.S.D. < 6%, n = 5) and sensitivity (ΔF = 34 Hz/1000 E. coli cells) as compared to existing manual method (R.S.D. = 19%, n = 5 and ΔF = 26 Hz/1000 E. coli cells, respectively). Down to 23 E. coli cells are detected, satisfying the HKEPD requirements for E. coli count in beach water. © 2006 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/168057
ISSN
2015 Impact Factor: 7.476
2015 SCImago Journal Rankings: 2.150
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSun, Hen_US
dc.contributor.authorZhang, Yen_US
dc.contributor.authorFung, Yen_US
dc.date.accessioned2012-10-08T03:14:37Z-
dc.date.available2012-10-08T03:14:37Z-
dc.date.issued2006en_US
dc.identifier.citationBiosensors And Bioelectronics, 2006, v. 22 n. 4 SPEC. ISS., p. 506-512en_US
dc.identifier.issn0956-5663en_US
dc.identifier.urihttp://hdl.handle.net/10722/168057-
dc.description.abstractA flow-through PQC/DNA biosensor system is developed by combining sequential flow polymerase chain reaction (PCR) products denaturing prior to piezoelectric quartz crystal (PQC) detection via hybridization of ssDNA. The PQC/DNA biosensor is fabricated based on complex formation of neutravidin/biotinylated probe in 0.2 M NaCl in TE buffer (10 mM Tris, 1 mM EDTA, pH 7.5). Results show that the coating fabricated provides a desirable quality with satisfactory performance. Its application for Escherichia coli detection under controlled flow at 0.02 mL/min for denaturing PCR products and 10 mL/min for transferring solution between reactors and delivering samples to detector to reduce rehybridization leads to significant improvement in repeatability (R.S.D. < 6%, n = 5) and sensitivity (ΔF = 34 Hz/1000 E. coli cells) as compared to existing manual method (R.S.D. = 19%, n = 5 and ΔF = 26 Hz/1000 E. coli cells, respectively). Down to 23 E. coli cells are detected, satisfying the HKEPD requirements for E. coli count in beach water. © 2006 Elsevier B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biosen_US
dc.relation.ispartofBiosensors and Bioelectronicsen_US
dc.subject.meshBiosensing Techniques - Instrumentation - Methodsen_US
dc.subject.meshColony Count, Microbial - Instrumentation - Methodsen_US
dc.subject.meshDna, Bacterial - Analysisen_US
dc.subject.meshElectrochemistry - Instrumentation - Methodsen_US
dc.subject.meshEnvironmental Monitoring - Instrumentationen_US
dc.subject.meshEquipment Designen_US
dc.subject.meshEquipment Failure Analysisen_US
dc.subject.meshEscherichia Coli - Genetics - Isolation & Purificationen_US
dc.subject.meshFlow Injection Analysis - Instrumentation - Methodsen_US
dc.subject.meshPolymerase Chain Reaction - Instrumentationen_US
dc.subject.meshReproducibility Of Resultsen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshWater Microbiologyen_US
dc.subject.meshWater Pollution - Analysisen_US
dc.titleFlow analysis coupled with PQC/DNA biosensor for assay of E. coli based on detecting DNA products from PCR amplificationen_US
dc.typeArticleen_US
dc.identifier.emailSun, H:sunhui@hku.hken_US
dc.identifier.emailFung, Y:ysfung@hku.hken_US
dc.identifier.authoritySun, H=rp00778en_US
dc.identifier.authorityFung, Y=rp00697en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.bios.2006.08.006en_US
dc.identifier.pmid16971109-
dc.identifier.scopuseid_2-s2.0-33749236579en_US
dc.identifier.hkuros134663-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33749236579&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume22en_US
dc.identifier.issue4 SPEC. ISS.en_US
dc.identifier.spage506en_US
dc.identifier.epage512en_US
dc.identifier.eissn1873-4235-
dc.identifier.isiWOS:000241547500008-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridSun, H=37032038600en_US
dc.identifier.scopusauthoridZhang, Y=49762465000en_US
dc.identifier.scopusauthoridFung, Y=13309754700en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats