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Article: COX-1 and -2 expressions in sex-related organs of neonatally estrogen-treated rats and in activated and nonactivated macrophage RAW264.7 cells with phytoestrogen

TitleCOX-1 and -2 expressions in sex-related organs of neonatally estrogen-treated rats and in activated and nonactivated macrophage RAW264.7 cells with phytoestrogen
Authors
Issue Date2006
Citation
Endocrine, 2006, v. 29 n. 1, p. 161-167 How to Cite?
AbstractCyclooxygenase (COX)-2 is an inducible isoform, expressed in inflamed leukocytes and cancer cells. It is known that estrogen causes prostate dysplasia, but little is known about COX-2 expression and its influence on male reproductivity. In this study, we show that COX-2 was abolished in the distal end of the vas deferens in neonatally estrogenized (diethylstilbestrol, NeoDES) Sprague-Dawley (SD) rats at age of 15 mo, but the control normal rats were found to remain constitutive expression at the same age, while the levels of COX-1 in these rats remained intact. Furthermore, BAX, an indicator of sperm quality, was observed in the endothelium of vas deferens and sperm of the aged rats. However, COX-2 was not detected in the inflamed lesions of NeoDES rat's prostate by immunohistochemistry. In addition to estrogen, hydroxymatairesinol (HMR), a phytoestrogen, was analyzed in vitro for possible regulation on COX-2. Through Western blot analysis, HMR was shown to have no inhibitory affect on COX-2 expression. These results indicated that estrogen treatment strongly influences the expression of COX-2 that is associated with fertility, but no induction of COX-2 by estrogen may not exclude COX-2′s role in prostatitis, and the anti-tumor mechanism of HMR largely remains elusive. © 2006 by Humana Press Inc. All rights of any nature whatsoever reserved.
Persistent Identifierhttp://hdl.handle.net/10722/168020
ISSN
2010 Impact Factor: 1.373
2015 SCImago Journal Rankings: 0.978
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLuo, Cen_US
dc.contributor.authorPeng, Yen_US
dc.contributor.authorSantti, Ren_US
dc.contributor.authorMing, LHen_US
dc.contributor.authorLin, MCen_US
dc.date.accessioned2012-10-08T03:14:12Z-
dc.date.available2012-10-08T03:14:12Z-
dc.date.issued2006en_US
dc.identifier.citationEndocrine, 2006, v. 29 n. 1, p. 161-167en_US
dc.identifier.issn0969-711Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/168020-
dc.description.abstractCyclooxygenase (COX)-2 is an inducible isoform, expressed in inflamed leukocytes and cancer cells. It is known that estrogen causes prostate dysplasia, but little is known about COX-2 expression and its influence on male reproductivity. In this study, we show that COX-2 was abolished in the distal end of the vas deferens in neonatally estrogenized (diethylstilbestrol, NeoDES) Sprague-Dawley (SD) rats at age of 15 mo, but the control normal rats were found to remain constitutive expression at the same age, while the levels of COX-1 in these rats remained intact. Furthermore, BAX, an indicator of sperm quality, was observed in the endothelium of vas deferens and sperm of the aged rats. However, COX-2 was not detected in the inflamed lesions of NeoDES rat's prostate by immunohistochemistry. In addition to estrogen, hydroxymatairesinol (HMR), a phytoestrogen, was analyzed in vitro for possible regulation on COX-2. Through Western blot analysis, HMR was shown to have no inhibitory affect on COX-2 expression. These results indicated that estrogen treatment strongly influences the expression of COX-2 that is associated with fertility, but no induction of COX-2 by estrogen may not exclude COX-2′s role in prostatitis, and the anti-tumor mechanism of HMR largely remains elusive. © 2006 by Humana Press Inc. All rights of any nature whatsoever reserved.en_US
dc.languageengen_US
dc.relation.ispartofEndocrineen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Newbornen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshCell Aging - Geneticsen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCyclooxygenase 1 - Analysis - Geneticsen_US
dc.subject.meshCyclooxygenase 2 - Analysis - Geneticsen_US
dc.subject.meshDiethylstilbestrol - Pharmacokineticsen_US
dc.subject.meshEnzyme Induction - Drug Effects - Physiologyen_US
dc.subject.meshEstrogens - Pharmacologyen_US
dc.subject.meshGene Expression Regulation, Enzymologic - Drug Effectsen_US
dc.subject.meshGenitalia, Male - Chemistry - Cytology - Drug Effectsen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshLignans - Pharmacologyen_US
dc.subject.meshLipopolysaccharides - Pharmacologyen_US
dc.subject.meshMacrophage Activation - Drug Effects - Physiologyen_US
dc.subject.meshMacrophages - Chemistry - Drug Effects - Physiologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Proteins - Analysis - Geneticsen_US
dc.subject.meshPhytoestrogens - Pharmacologyen_US
dc.subject.meshProstate - Chemistry - Cytology - Drug Effectsen_US
dc.subject.meshRatsen_US
dc.subject.meshSpermatozoa - Chemistry - Cytology - Drug Effectsen_US
dc.subject.meshVas Deferens - Chemistry - Cytology - Drug Effectsen_US
dc.subject.meshBcl-2-Associated X Protein - Analysisen_US
dc.titleCOX-1 and -2 expressions in sex-related organs of neonatally estrogen-treated rats and in activated and nonactivated macrophage RAW264.7 cells with phytoestrogenen_US
dc.typeArticleen_US
dc.identifier.emailLin, MC:mcllin@hkucc.hku.hken_US
dc.identifier.authorityLin, MC=rp00746en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1385/ENDO:29:1:161en_US
dc.identifier.pmid16622306-
dc.identifier.scopuseid_2-s2.0-33646895156en_US
dc.identifier.hkuros115046-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33646895156&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume29en_US
dc.identifier.issue1en_US
dc.identifier.spage161en_US
dc.identifier.epage167en_US
dc.identifier.isiWOS:000236772000021-
dc.identifier.scopusauthoridLuo, C=26324947200en_US
dc.identifier.scopusauthoridPeng, Y=7403419265en_US
dc.identifier.scopusauthoridSantti, R=7005758859en_US
dc.identifier.scopusauthoridMing, LH=13612836600en_US
dc.identifier.scopusauthoridLin, MC=7404816359en_US

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