Article: Involvement of NF-κB and c-myc signaling pathways in the apoptosis of HL-60 cells induced by alkaloids of Tripterygium hypoglaucum (levl.) Hutch

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TitleInvolvement of NF-κB and c-myc signaling pathways in the apoptosis of HL-60 cells induced by alkaloids of Tripterygium hypoglaucum (levl.) Hutch
AuthorsZhuang, WJ1
Fong, CC1
Cao, J3
Ao, L3
Leung, CH1
Cheung, HY1
Xiao, PG2
Fong, WF1
Yang, MS1
Issue Date2004
PublisherUrban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/phytomed
CitationPhytomedicine, 2004, v. 11 n. 4, p. 295-302 [How to Cite?]
DOI: http://dx.doi.org/10.1078/0944711041495128
AbstractTripterygium hypoglaucum (levl.) Hutch (Celastraceae) (THH) root is a Chinese medicinal herb commonly used for treating autoimmune diseases. In the present study, alkaloids of THH were prepared and their cytotoxicity against the HL-60 cell was investigated. THH-induced apoptosis was observed using flow cytometry, confocal fluorescence microscope, and DNA laddering and caspase assays. The molecular mechanism involved in the induction of HL-60 cell apoptosis by THH alkaloids was examined using cDNA microarrays containing 3000 human genes derived from a leukocyte cDNA library. Sixteen genes were identified to be differentially expressed in HL-60 cells upon THH treatment. Several genes related to the NF-κB signaling pathway and cell apoptosis (such as NFKBIB, PRG1 and B2M) were up-regulated. In addition, c-myc binding protein and apoptosis-related cysteine proteases caspase-3 and caspase-8 were also regulated. The changes in c-Myc RNA expression and c-myc protein level were further confirmed by RT-PCR and Western blot analysis. The results demonstrated that THH alkaloids induced apoptosis of HL-60 cells though c-myc and NF-κB signaling pathways.
ISSN0944-7113
2011 Impact Factor: 3.268
2011 SCImago Journal Rankings: 0.133
DOIhttp://dx.doi.org/10.1078/0944711041495128
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorZhuang, WJ
dc.contributor.authorFong, CC
dc.contributor.authorCao, J
dc.contributor.authorAo, L
dc.contributor.authorLeung, CH
dc.contributor.authorCheung, HY
dc.contributor.authorXiao, PG
dc.contributor.authorFong, WF
dc.contributor.authorYang, MS
dc.date.accessioned2012-10-08T03:13:22Z
dc.date.available2012-10-08T03:13:22Z
dc.date.issued2004
dc.description.abstractTripterygium hypoglaucum (levl.) Hutch (Celastraceae) (THH) root is a Chinese medicinal herb commonly used for treating autoimmune diseases. In the present study, alkaloids of THH were prepared and their cytotoxicity against the HL-60 cell was investigated. THH-induced apoptosis was observed using flow cytometry, confocal fluorescence microscope, and DNA laddering and caspase assays. The molecular mechanism involved in the induction of HL-60 cell apoptosis by THH alkaloids was examined using cDNA microarrays containing 3000 human genes derived from a leukocyte cDNA library. Sixteen genes were identified to be differentially expressed in HL-60 cells upon THH treatment. Several genes related to the NF-κB signaling pathway and cell apoptosis (such as NFKBIB, PRG1 and B2M) were up-regulated. In addition, c-myc binding protein and apoptosis-related cysteine proteases caspase-3 and caspase-8 were also regulated. The changes in c-Myc RNA expression and c-myc protein level were further confirmed by RT-PCR and Western blot analysis. The results demonstrated that THH alkaloids induced apoptosis of HL-60 cells though c-myc and NF-κB signaling pathways.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationPhytomedicine, 2004, v. 11 n. 4, p. 295-302 [How to Cite?]
DOI: http://dx.doi.org/10.1078/0944711041495128
dc.identifier.citeulike17692
dc.identifier.doihttp://dx.doi.org/10.1078/0944711041495128
dc.identifier.epage302
dc.identifier.issn0944-7113
2011 Impact Factor: 3.268
2011 SCImago Journal Rankings: 0.133
dc.identifier.issue4
dc.identifier.pmid15185841
dc.identifier.scopuseid_2-s2.0-2442637447
dc.identifier.spage295
dc.identifier.urihttp://hdl.handle.net/10722/167954
dc.identifier.volume11
dc.languageeng
dc.publisherUrban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/phytomed
dc.publisher.placeGermany
dc.relation.ispartofPhytomedicine
dc.relation.referencesReferences in Scopus
dc.subject.meshAntineoplastic Agents, Phytogenic - Administration & Dosage - Pharmacology - Therapeutic Use
dc.subject.meshApoptosis - Drug Effects
dc.subject.meshBlotting, Western
dc.subject.meshDna, Neoplasm - Drug Effects
dc.subject.meshFlow Cytometry
dc.subject.meshHl-60 Cells - Drug Effects
dc.subject.meshHumans
dc.subject.meshMicroscopy, Confocal
dc.subject.meshNf-Kappa B - Metabolism
dc.subject.meshPhytotherapy
dc.subject.meshPlant Extracts - Administration & Dosage - Pharmacology - Therapeutic Use
dc.subject.meshPlant Roots
dc.subject.meshProto-Oncogene Proteins C-Myc - Metabolism
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
dc.subject.meshTripterygium
dc.titleInvolvement of NF-κB and c-myc signaling pathways in the apoptosis of HL-60 cells induced by alkaloids of Tripterygium hypoglaucum (levl.) Hutch
dc.typeArticle
Author Affiliations
  1. City University of Hong Kong
  2. Chinese Academy of Medical Sciences
  3. Third Military Medical University