File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Involvement of NF-κB and c-myc signaling pathways in the apoptosis of HL-60 cells induced by alkaloids of Tripterygium hypoglaucum (levl.) Hutch

TitleInvolvement of NF-κB and c-myc signaling pathways in the apoptosis of HL-60 cells induced by alkaloids of Tripterygium hypoglaucum (levl.) Hutch
Authors
Issue Date2004
PublisherUrban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/phytomed
Citation
Phytomedicine, 2004, v. 11 n. 4, p. 295-302 How to Cite?
AbstractTripterygium hypoglaucum (levl.) Hutch (Celastraceae) (THH) root is a Chinese medicinal herb commonly used for treating autoimmune diseases. In the present study, alkaloids of THH were prepared and their cytotoxicity against the HL-60 cell was investigated. THH-induced apoptosis was observed using flow cytometry, confocal fluorescence microscope, and DNA laddering and caspase assays. The molecular mechanism involved in the induction of HL-60 cell apoptosis by THH alkaloids was examined using cDNA microarrays containing 3000 human genes derived from a leukocyte cDNA library. Sixteen genes were identified to be differentially expressed in HL-60 cells upon THH treatment. Several genes related to the NF-κB signaling pathway and cell apoptosis (such as NFKBIB, PRG1 and B2M) were up-regulated. In addition, c-myc binding protein and apoptosis-related cysteine proteases caspase-3 and caspase-8 were also regulated. The changes in c-Myc RNA expression and c-myc protein level were further confirmed by RT-PCR and Western blot analysis. The results demonstrated that THH alkaloids induced apoptosis of HL-60 cells though c-myc and NF-κB signaling pathways.
Persistent Identifierhttp://hdl.handle.net/10722/167954
ISSN
2014 Impact Factor: 3.126
2013 SCImago Journal Rankings: 1.023
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhuang, WJen_US
dc.contributor.authorFong, CCen_US
dc.contributor.authorCao, Jen_US
dc.contributor.authorAo, Len_US
dc.contributor.authorLeung, CHen_US
dc.contributor.authorCheung, HYen_US
dc.contributor.authorXiao, PGen_US
dc.contributor.authorFong, WFen_US
dc.contributor.authorYang, MSen_US
dc.date.accessioned2012-10-08T03:13:22Z-
dc.date.available2012-10-08T03:13:22Z-
dc.date.issued2004en_US
dc.identifier.citationPhytomedicine, 2004, v. 11 n. 4, p. 295-302en_US
dc.identifier.issn0944-7113en_US
dc.identifier.urihttp://hdl.handle.net/10722/167954-
dc.description.abstractTripterygium hypoglaucum (levl.) Hutch (Celastraceae) (THH) root is a Chinese medicinal herb commonly used for treating autoimmune diseases. In the present study, alkaloids of THH were prepared and their cytotoxicity against the HL-60 cell was investigated. THH-induced apoptosis was observed using flow cytometry, confocal fluorescence microscope, and DNA laddering and caspase assays. The molecular mechanism involved in the induction of HL-60 cell apoptosis by THH alkaloids was examined using cDNA microarrays containing 3000 human genes derived from a leukocyte cDNA library. Sixteen genes were identified to be differentially expressed in HL-60 cells upon THH treatment. Several genes related to the NF-κB signaling pathway and cell apoptosis (such as NFKBIB, PRG1 and B2M) were up-regulated. In addition, c-myc binding protein and apoptosis-related cysteine proteases caspase-3 and caspase-8 were also regulated. The changes in c-Myc RNA expression and c-myc protein level were further confirmed by RT-PCR and Western blot analysis. The results demonstrated that THH alkaloids induced apoptosis of HL-60 cells though c-myc and NF-κB signaling pathways.en_US
dc.languageengen_US
dc.publisherUrban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/phytomeden_US
dc.relation.ispartofPhytomedicineen_US
dc.subject.meshAntineoplastic Agents, Phytogenic - Administration & Dosage - Pharmacology - Therapeutic Useen_US
dc.subject.meshApoptosis - Drug Effectsen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshDna, Neoplasm - Drug Effectsen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshHl-60 Cells - Drug Effectsen_US
dc.subject.meshHumansen_US
dc.subject.meshMicroscopy, Confocalen_US
dc.subject.meshNf-Kappa B - Metabolismen_US
dc.subject.meshPhytotherapyen_US
dc.subject.meshPlant Extracts - Administration & Dosage - Pharmacology - Therapeutic Useen_US
dc.subject.meshPlant Rootsen_US
dc.subject.meshProto-Oncogene Proteins C-Myc - Metabolismen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshTripterygiumen_US
dc.titleInvolvement of NF-κB and c-myc signaling pathways in the apoptosis of HL-60 cells induced by alkaloids of Tripterygium hypoglaucum (levl.) Hutchen_US
dc.typeArticleen_US
dc.identifier.emailLeung, CH:duncanl@hkucc.hku.hken_US
dc.identifier.authorityLeung, CH=rp00730en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1078/0944711041495128en_US
dc.identifier.pmid15185841en_US
dc.identifier.scopuseid_2-s2.0-2442637447en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-2442637447&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume11en_US
dc.identifier.issue4en_US
dc.identifier.spage295en_US
dc.identifier.epage302en_US
dc.identifier.isiWOS:000221891400003-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridZhuang, WJ=7103154978en_US
dc.identifier.scopusauthoridFong, CC=11739503800en_US
dc.identifier.scopusauthoridCao, J=26643166500en_US
dc.identifier.scopusauthoridAo, L=15055316500en_US
dc.identifier.scopusauthoridLeung, CH=7402612570en_US
dc.identifier.scopusauthoridCheung, HY=7201839371en_US
dc.identifier.scopusauthoridXiao, PG=7103088959en_US
dc.identifier.scopusauthoridFong, WF=7102816013en_US
dc.identifier.scopusauthoridYang, MS=7404925734en_US
dc.identifier.citeulike17692-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats