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Article: Involvement of NF-κB and c-myc signaling pathways in the apoptosis of HL-60 cells induced by alkaloids of Tripterygium hypoglaucum (levl.) Hutch
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TitleInvolvement of NF-κB and c-myc signaling pathways in the apoptosis of HL-60 cells induced by alkaloids of Tripterygium hypoglaucum (levl.) Hutch
 
AuthorsZhuang, WJ1
Fong, CC1
Cao, J3
Ao, L3
Leung, CH1
Cheung, HY1
Xiao, PG2
Fong, WF1
Yang, MS1
 
Issue Date2004
 
PublisherUrban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/phytomed
 
CitationPhytomedicine, 2004, v. 11 n. 4, p. 295-302 [How to Cite?]
DOI: http://dx.doi.org/10.1078/0944711041495128
 
AbstractTripterygium hypoglaucum (levl.) Hutch (Celastraceae) (THH) root is a Chinese medicinal herb commonly used for treating autoimmune diseases. In the present study, alkaloids of THH were prepared and their cytotoxicity against the HL-60 cell was investigated. THH-induced apoptosis was observed using flow cytometry, confocal fluorescence microscope, and DNA laddering and caspase assays. The molecular mechanism involved in the induction of HL-60 cell apoptosis by THH alkaloids was examined using cDNA microarrays containing 3000 human genes derived from a leukocyte cDNA library. Sixteen genes were identified to be differentially expressed in HL-60 cells upon THH treatment. Several genes related to the NF-κB signaling pathway and cell apoptosis (such as NFKBIB, PRG1 and B2M) were up-regulated. In addition, c-myc binding protein and apoptosis-related cysteine proteases caspase-3 and caspase-8 were also regulated. The changes in c-Myc RNA expression and c-myc protein level were further confirmed by RT-PCR and Western blot analysis. The results demonstrated that THH alkaloids induced apoptosis of HL-60 cells though c-myc and NF-κB signaling pathways.
 
ISSN0944-7113
2012 Impact Factor: 2.972
2012 SCImago Journal Rankings: 0.952
 
DOIhttp://dx.doi.org/10.1078/0944711041495128
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorZhuang, WJ
 
dc.contributor.authorFong, CC
 
dc.contributor.authorCao, J
 
dc.contributor.authorAo, L
 
dc.contributor.authorLeung, CH
 
dc.contributor.authorCheung, HY
 
dc.contributor.authorXiao, PG
 
dc.contributor.authorFong, WF
 
dc.contributor.authorYang, MS
 
dc.date.accessioned2012-10-08T03:13:22Z
 
dc.date.available2012-10-08T03:13:22Z
 
dc.date.issued2004
 
dc.description.abstractTripterygium hypoglaucum (levl.) Hutch (Celastraceae) (THH) root is a Chinese medicinal herb commonly used for treating autoimmune diseases. In the present study, alkaloids of THH were prepared and their cytotoxicity against the HL-60 cell was investigated. THH-induced apoptosis was observed using flow cytometry, confocal fluorescence microscope, and DNA laddering and caspase assays. The molecular mechanism involved in the induction of HL-60 cell apoptosis by THH alkaloids was examined using cDNA microarrays containing 3000 human genes derived from a leukocyte cDNA library. Sixteen genes were identified to be differentially expressed in HL-60 cells upon THH treatment. Several genes related to the NF-κB signaling pathway and cell apoptosis (such as NFKBIB, PRG1 and B2M) were up-regulated. In addition, c-myc binding protein and apoptosis-related cysteine proteases caspase-3 and caspase-8 were also regulated. The changes in c-Myc RNA expression and c-myc protein level were further confirmed by RT-PCR and Western blot analysis. The results demonstrated that THH alkaloids induced apoptosis of HL-60 cells though c-myc and NF-κB signaling pathways.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationPhytomedicine, 2004, v. 11 n. 4, p. 295-302 [How to Cite?]
DOI: http://dx.doi.org/10.1078/0944711041495128
 
dc.identifier.citeulike17692
 
dc.identifier.doihttp://dx.doi.org/10.1078/0944711041495128
 
dc.identifier.epage302
 
dc.identifier.issn0944-7113
2012 Impact Factor: 2.972
2012 SCImago Journal Rankings: 0.952
 
dc.identifier.issue4
 
dc.identifier.pmid15185841
 
dc.identifier.scopuseid_2-s2.0-2442637447
 
dc.identifier.spage295
 
dc.identifier.urihttp://hdl.handle.net/10722/167954
 
dc.identifier.volume11
 
dc.languageeng
 
dc.publisherUrban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/phytomed
 
dc.publisher.placeGermany
 
dc.relation.ispartofPhytomedicine
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAntineoplastic Agents, Phytogenic - Administration & Dosage - Pharmacology - Therapeutic Use
 
dc.subject.meshApoptosis - Drug Effects
 
dc.subject.meshBlotting, Western
 
dc.subject.meshDna, Neoplasm - Drug Effects
 
dc.subject.meshFlow Cytometry
 
dc.subject.meshHl-60 Cells - Drug Effects
 
dc.subject.meshHumans
 
dc.subject.meshMicroscopy, Confocal
 
dc.subject.meshNf-Kappa B - Metabolism
 
dc.subject.meshPhytotherapy
 
dc.subject.meshPlant Extracts - Administration & Dosage - Pharmacology - Therapeutic Use
 
dc.subject.meshPlant Roots
 
dc.subject.meshProto-Oncogene Proteins C-Myc - Metabolism
 
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
 
dc.subject.meshTripterygium
 
dc.titleInvolvement of NF-κB and c-myc signaling pathways in the apoptosis of HL-60 cells induced by alkaloids of Tripterygium hypoglaucum (levl.) Hutch
 
dc.typeArticle
 
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<contributor.author>Cheung, HY</contributor.author>
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<description.abstract>Tripterygium hypoglaucum (levl.) Hutch (Celastraceae) (THH) root is a Chinese medicinal herb commonly used for treating autoimmune diseases. In the present study, alkaloids of THH were prepared and their cytotoxicity against the HL-60 cell was investigated. THH-induced apoptosis was observed using flow cytometry, confocal fluorescence microscope, and DNA laddering and caspase assays. The molecular mechanism involved in the induction of HL-60 cell apoptosis by THH alkaloids was examined using cDNA microarrays containing 3000 human genes derived from a leukocyte cDNA library. Sixteen genes were identified to be differentially expressed in HL-60 cells upon THH treatment. Several genes related to the NF-&#954;B signaling pathway and cell apoptosis (such as NFKBIB, PRG1 and B2M) were up-regulated. In addition, c-myc binding protein and apoptosis-related cysteine proteases caspase-3 and caspase-8 were also regulated. The changes in c-Myc RNA expression and c-myc protein level were further confirmed by RT-PCR and Western blot analysis. The results demonstrated that THH alkaloids induced apoptosis of HL-60 cells though c-myc and NF-&#954;B signaling pathways.</description.abstract>
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Author Affiliations
  1. City University of Hong Kong
  2. Chinese Academy of Medical Sciences
  3. Third Military Medical University