File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1002/cbic.200390015
- Scopus: eid_2-s2.0-0037415003
- PMID: 12512077
- WOS: WOS:000180311600007
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Synthesis and biological activity of a platinum(II) 6-Phenyl-2,2′-bipyridine complex and its dimeric analogue
Title | Synthesis and biological activity of a platinum(II) 6-Phenyl-2,2′-bipyridine complex and its dimeric analogue |
---|---|
Authors | |
Keywords | Antitumor agents Bioinorganic chemistry DNA Intercalation Platinum |
Issue Date | 2003 |
Publisher | Wiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.chembiochem.com |
Citation | Chembiochem, 2003, v. 4 n. 1, p. 62-68 How to Cite? |
Abstract | We have synthesized (pyridyl)-(6-phenyl-2,2′-bipyridine)platinum(II) hexafluorophosphate (1) and its corresponding dimer, μ-N,N′-bis(isonicotinyl)-1, 6-hexanediamino bis-[6-phenyl-2,2′-bipyridine-platinum(II)] dichloride (2). The DNA binding constants of 1 and 2 at 20°C were determined by absorption titration to be 2.25 × 104 M-1 and 3.07 × 106 M-1, respectively. Compound 1 showed an AT preference, while 2 had no base preference. The binding site sizes of 2 for [poly(dA-dT)]2, calf thymus DNA (ctDNA), and [poly(dG-dC)]2, as determined by fluorescence titration, were 6.6, 4.0, and 2.8 bp, respectively. Compound 2 probably bound to [poly(dA-dT)]2 through bisintercalation, and to [poly(dG-dC)]2 by monointercalation. Binding of DNA by both complexes is favorable, since the binding free energies of 1 and 2 were estimated to be -5.8 and -8.7 kcal mol-1, respectively. The results of viscosity measurements and gel mobility shift assay demonstrated that binding of 1 and 2 caused DNA lengthening. The cytotoxicities of the complexes in various human cancer cell lines were determined by MTT assay. Complex 2 exhibited cytotoxicity comparable to that of cisplatin, and was more toxic than I by an order of magnitude. |
Persistent Identifier | http://hdl.handle.net/10722/167775 |
ISSN | 2023 Impact Factor: 2.6 2023 SCImago Journal Rankings: 0.809 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chan, HL | en_US |
dc.contributor.author | Ma, DL | en_US |
dc.contributor.author | Yang, M | en_US |
dc.contributor.author | Che, CM | en_US |
dc.date.accessioned | 2012-10-08T03:11:25Z | - |
dc.date.available | 2012-10-08T03:11:25Z | - |
dc.date.issued | 2003 | en_US |
dc.identifier.citation | Chembiochem, 2003, v. 4 n. 1, p. 62-68 | en_US |
dc.identifier.issn | 1439-4227 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/167775 | - |
dc.description.abstract | We have synthesized (pyridyl)-(6-phenyl-2,2′-bipyridine)platinum(II) hexafluorophosphate (1) and its corresponding dimer, μ-N,N′-bis(isonicotinyl)-1, 6-hexanediamino bis-[6-phenyl-2,2′-bipyridine-platinum(II)] dichloride (2). The DNA binding constants of 1 and 2 at 20°C were determined by absorption titration to be 2.25 × 104 M-1 and 3.07 × 106 M-1, respectively. Compound 1 showed an AT preference, while 2 had no base preference. The binding site sizes of 2 for [poly(dA-dT)]2, calf thymus DNA (ctDNA), and [poly(dG-dC)]2, as determined by fluorescence titration, were 6.6, 4.0, and 2.8 bp, respectively. Compound 2 probably bound to [poly(dA-dT)]2 through bisintercalation, and to [poly(dG-dC)]2 by monointercalation. Binding of DNA by both complexes is favorable, since the binding free energies of 1 and 2 were estimated to be -5.8 and -8.7 kcal mol-1, respectively. The results of viscosity measurements and gel mobility shift assay demonstrated that binding of 1 and 2 caused DNA lengthening. The cytotoxicities of the complexes in various human cancer cell lines were determined by MTT assay. Complex 2 exhibited cytotoxicity comparable to that of cisplatin, and was more toxic than I by an order of magnitude. | en_US |
dc.language | eng | en_US |
dc.publisher | Wiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.chembiochem.com | en_US |
dc.relation.ispartof | ChemBioChem | en_US |
dc.subject | Antitumor agents | - |
dc.subject | Bioinorganic chemistry | - |
dc.subject | DNA | - |
dc.subject | Intercalation | - |
dc.subject | Platinum | - |
dc.subject.mesh | Algorithms | en_US |
dc.subject.mesh | Antineoplastic Agents - Chemical Synthesis - Pharmacology | en_US |
dc.subject.mesh | Dna - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Dna Fragmentation - Drug Effects | en_US |
dc.subject.mesh | Drug Screening Assays, Antitumor | en_US |
dc.subject.mesh | Electrophoretic Mobility Shift Assay | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Indicators And Reagents | en_US |
dc.subject.mesh | Nucleic Acid Conformation | en_US |
dc.subject.mesh | Organoplatinum Compounds - Chemical Synthesis - Pharmacology | en_US |
dc.subject.mesh | Pyridines - Chemical Synthesis - Pharmacology | en_US |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | en_US |
dc.subject.mesh | Spectrometry, Fluorescence | en_US |
dc.subject.mesh | Spectrophotometry, Ultraviolet | en_US |
dc.subject.mesh | Tetrazolium Salts | en_US |
dc.subject.mesh | Thermodynamics | en_US |
dc.subject.mesh | Thiazoles | en_US |
dc.subject.mesh | Tumor Cells, Cultured | en_US |
dc.subject.mesh | Viscosity | en_US |
dc.title | Synthesis and biological activity of a platinum(II) 6-Phenyl-2,2′-bipyridine complex and its dimeric analogue | en_US |
dc.type | Article | en_US |
dc.identifier.email | Ma, DL:edmondma@hku.hk | en_US |
dc.identifier.email | Che, CM:cmche@hku.hk | en_US |
dc.identifier.authority | Ma, DL=rp00760 | en_US |
dc.identifier.authority | Che, CM=rp00670 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1002/cbic.200390015 | en_US |
dc.identifier.pmid | 12512077 | - |
dc.identifier.scopus | eid_2-s2.0-0037415003 | en_US |
dc.identifier.hkuros | 83820 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0037415003&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 4 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 62 | en_US |
dc.identifier.epage | 68 | en_US |
dc.identifier.isi | WOS:000180311600007 | - |
dc.publisher.place | Germany | en_US |
dc.identifier.scopusauthorid | Chan, HL=7403402315 | en_US |
dc.identifier.scopusauthorid | Ma, DL=7402075538 | en_US |
dc.identifier.scopusauthorid | Yang, M=35204210300 | en_US |
dc.identifier.scopusauthorid | Che, CM=7102442791 | en_US |
dc.identifier.issnl | 1439-4227 | - |