File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Sequencing of argentinated peptides by means of matrix-assisted laser desorption/ionization tandem mass spectrometry

TitleSequencing of argentinated peptides by means of matrix-assisted laser desorption/ionization tandem mass spectrometry
Authors
Issue Date2002
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/ac
Citation
Analytical Chemistry, 2002, v. 74 n. 9, p. 2072-2082 How to Cite?
AbstractArgentinated peptide ions are formed in abundance under matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) conditions in the presence of Ag + ions. These argentinated peptide ions are fragmented facilely under MALDI-MS/MS conditions to yield [b n + OH + Ag] +, [b n - H + Ag] + and [a n - H + Ag] + ions that are indicative of the C-terminal sequence. These observations parallel those made earlier under electrospray MS conditions (Chu, I. K.; Guo, X.; Lau, T.-C.; Siu, K. W. M. Anal. Chem. 1999, 71, 2364-2372). A mixed protonated and argentinated tryptic peptide map was generated from 37 fmol of bovine serum albumin (BSA) using MALDI-MS. MALDI-MS/MS data from four argentinated peptides at a protein amount of 350 fmol unambiguously identified the protein as BSA. Sequence-tag analysis of two argentinated tryptic peptides was used to identify unambiguously myocyte enhancer factor 2A, which had been recombinantly expressed in a bacterial cell line.
Persistent Identifierhttp://hdl.handle.net/10722/167736
ISSN
2015 Impact Factor: 5.886
2015 SCImago Journal Rankings: 2.369
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChu, IKen_US
dc.contributor.authorCox, DMen_US
dc.contributor.authorGuo, Xen_US
dc.contributor.authorKireeva, Ien_US
dc.contributor.authorLau, TCen_US
dc.contributor.authorMcdermott, JCen_US
dc.contributor.authorSiu, KWMen_US
dc.date.accessioned2012-10-08T03:10:45Z-
dc.date.available2012-10-08T03:10:45Z-
dc.date.issued2002en_US
dc.identifier.citationAnalytical Chemistry, 2002, v. 74 n. 9, p. 2072-2082en_US
dc.identifier.issn0003-2700en_US
dc.identifier.urihttp://hdl.handle.net/10722/167736-
dc.description.abstractArgentinated peptide ions are formed in abundance under matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) conditions in the presence of Ag + ions. These argentinated peptide ions are fragmented facilely under MALDI-MS/MS conditions to yield [b n + OH + Ag] +, [b n - H + Ag] + and [a n - H + Ag] + ions that are indicative of the C-terminal sequence. These observations parallel those made earlier under electrospray MS conditions (Chu, I. K.; Guo, X.; Lau, T.-C.; Siu, K. W. M. Anal. Chem. 1999, 71, 2364-2372). A mixed protonated and argentinated tryptic peptide map was generated from 37 fmol of bovine serum albumin (BSA) using MALDI-MS. MALDI-MS/MS data from four argentinated peptides at a protein amount of 350 fmol unambiguously identified the protein as BSA. Sequence-tag analysis of two argentinated tryptic peptides was used to identify unambiguously myocyte enhancer factor 2A, which had been recombinantly expressed in a bacterial cell line.en_US
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/acen_US
dc.relation.ispartofAnalytical Chemistryen_US
dc.titleSequencing of argentinated peptides by means of matrix-assisted laser desorption/ionization tandem mass spectrometryen_US
dc.typeArticleen_US
dc.identifier.emailChu, IK:ivankchu@hku.hken_US
dc.identifier.authorityChu, IK=rp00683en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/ac0111006en_US
dc.identifier.pmid12033309-
dc.identifier.scopuseid_2-s2.0-0036558177en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036558177&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume74en_US
dc.identifier.issue9en_US
dc.identifier.spage2072en_US
dc.identifier.epage2082en_US
dc.identifier.isiWOS:000175358800047-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChu, IK=7103327484en_US
dc.identifier.scopusauthoridCox, DM=35434137900en_US
dc.identifier.scopusauthoridGuo, X=36725887400en_US
dc.identifier.scopusauthoridKireeva, I=7005205003en_US
dc.identifier.scopusauthoridLau, TC=7102222310en_US
dc.identifier.scopusauthoridMcDermott, JC=26655975900en_US
dc.identifier.scopusauthoridSiu, KWM=8967015800en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats