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Article: PKCδ-dependent deubiquitination and stabilization of Gadd45 in A431 cells overexposed to EGF

TitlePKCδ-dependent deubiquitination and stabilization of Gadd45 in A431 cells overexposed to EGF
Authors
KeywordsA431 Cell
Epidermal Growth Factor
Gadd45
Protein Kinase C
Ubiquitination
Issue Date2001
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2001, v. 285 n. 2, p. 283-288 How to Cite?
AbstractEpidermal growth factor (EGF) receptor-overexpressing p53-deficient A431 cells response to toxic dose of EGF by G1 arrest and apoptosis was studied. We previously reported an increased expression of growth arrest and DNA-damage-inducible gene, Gadd45, in EGF-overexposed A431 cells. The mechanism for this induction was increased half-lives of mRNA and protein. In this study, using phorbol ester (a PKC activator) and specific inhibitors of PKC isoforms, we showed that protein kinase C-δ (PKCδ) was involved in the increase of Gadd45 protein stability. We further demonstrated that Gadd45 is ubiquitinated and is regulated by proteolysis. While EGF induced ubiquitination of total cellular proteins, there was a decrease in Gadd45 ubiquitination, which could be inhibited by Rottlerin, a PKCδ-specific inhibitor. These results suggest that an increase in Gadd45 stability may involve PKCδ-dependent ubiquitin - proteasome pathway. © 2001 Academic Press.
Persistent Identifierhttp://hdl.handle.net/10722/167671
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, CHen_US
dc.contributor.authorLam, Wen_US
dc.contributor.authorZhuang, WJen_US
dc.contributor.authorWang, NSen_US
dc.contributor.authorFong, WFen_US
dc.date.accessioned2012-10-08T03:09:44Z-
dc.date.available2012-10-08T03:09:44Z-
dc.date.issued2001en_US
dc.identifier.citationBiochemical And Biophysical Research Communications, 2001, v. 285 n. 2, p. 283-288en_US
dc.identifier.issn0006-291Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/167671-
dc.description.abstractEpidermal growth factor (EGF) receptor-overexpressing p53-deficient A431 cells response to toxic dose of EGF by G1 arrest and apoptosis was studied. We previously reported an increased expression of growth arrest and DNA-damage-inducible gene, Gadd45, in EGF-overexposed A431 cells. The mechanism for this induction was increased half-lives of mRNA and protein. In this study, using phorbol ester (a PKC activator) and specific inhibitors of PKC isoforms, we showed that protein kinase C-δ (PKCδ) was involved in the increase of Gadd45 protein stability. We further demonstrated that Gadd45 is ubiquitinated and is regulated by proteolysis. While EGF induced ubiquitination of total cellular proteins, there was a decrease in Gadd45 ubiquitination, which could be inhibited by Rottlerin, a PKCδ-specific inhibitor. These results suggest that an increase in Gadd45 stability may involve PKCδ-dependent ubiquitin - proteasome pathway. © 2001 Academic Press.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_US
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_US
dc.subjectA431 Cellen_US
dc.subjectEpidermal Growth Factoren_US
dc.subjectGadd45en_US
dc.subjectProtein Kinase Cen_US
dc.subjectUbiquitinationen_US
dc.titlePKCδ-dependent deubiquitination and stabilization of Gadd45 in A431 cells overexposed to EGFen_US
dc.typeArticleen_US
dc.identifier.emailLeung, CH:duncanl@hkucc.hku.hken_US
dc.identifier.authorityLeung, CH=rp00730en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/bbrc.2001.5164en_US
dc.identifier.pmid11444839-
dc.identifier.scopuseid_2-s2.0-0034741278en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034741278&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume285en_US
dc.identifier.issue2en_US
dc.identifier.spage283en_US
dc.identifier.epage288en_US
dc.identifier.isiWOS:000170020900020-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLeung, CH=7402612570en_US
dc.identifier.scopusauthoridLam, W=7203021943en_US
dc.identifier.scopusauthoridZhuang, WJ=7103154978en_US
dc.identifier.scopusauthoridWang, NS=36985478700en_US
dc.identifier.scopusauthoridFong, WF=7102816013en_US

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