File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Identification of an erythroid active element in the transferrin receptor gene

TitleIdentification of an erythroid active element in the transferrin receptor gene
Authors
Issue Date2000
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 2000, v. 275 n. 31, p. 24185-24190 How to Cite?
AbstractHemoglobin synthesis consumes most of the iron that is taken up by cells from plasma transferrin, and this process requires very high expression of transferrin receptors (TfR) at the membranes of erythroid cells. Studies in our and other laboratories indicate that a dramatic increase in TfR levels during erythroid differentiation occurs at the transcriptional level. In this study, we investigated the transcriptional regulation of the TfR in terms of its promoter activity and DNA-protein binding in murine erythroleukemia cells. Reporter gene assays revealed that the TfR promoter activity was stimulated 6-8-fold in murine erythroleukemia cells induced to differentiate into hemoglobin-synthesizing cells by either Me2SO or N,N'-hexamethylene-bis-acetamide. A minimal region (-118 to +14) was required for the differentiation-induced promoter activity. Mutation of either an Ets-binding site or an activator protein-1/cyclic AMP-response element-like motif within this region, but not disruption of the adjacent GC-rich/specificity protein-1 sequence, inhibited the inducible promoter activity. Electrophoresis mobility shift assays suggest that the cyclic AMP-response element-binding proteins/activating transcription factor-like factors and Ets-like factors bind constitutively to this bipartite element. Upon induction of differentiation, a shift in the pattern of the cyclic AMP-response element-binding protein/activating transcription factor-like binding factors was observed. Our data indicate that the TfR gene promoter contains an erythroid active element that stimulates the receptor gene transcription upon induction of hemoglobin synthesis.
Persistent Identifierhttp://hdl.handle.net/10722/167657
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
References

 

DC FieldValueLanguage
dc.contributor.authorLok, CNen_US
dc.contributor.authorPonka, Pen_US
dc.date.accessioned2012-10-08T03:09:31Z-
dc.date.available2012-10-08T03:09:31Z-
dc.date.issued2000en_US
dc.identifier.citationJournal Of Biological Chemistry, 2000, v. 275 n. 31, p. 24185-24190en_US
dc.identifier.issn0021-9258en_US
dc.identifier.urihttp://hdl.handle.net/10722/167657-
dc.description.abstractHemoglobin synthesis consumes most of the iron that is taken up by cells from plasma transferrin, and this process requires very high expression of transferrin receptors (TfR) at the membranes of erythroid cells. Studies in our and other laboratories indicate that a dramatic increase in TfR levels during erythroid differentiation occurs at the transcriptional level. In this study, we investigated the transcriptional regulation of the TfR in terms of its promoter activity and DNA-protein binding in murine erythroleukemia cells. Reporter gene assays revealed that the TfR promoter activity was stimulated 6-8-fold in murine erythroleukemia cells induced to differentiate into hemoglobin-synthesizing cells by either Me2SO or N,N'-hexamethylene-bis-acetamide. A minimal region (-118 to +14) was required for the differentiation-induced promoter activity. Mutation of either an Ets-binding site or an activator protein-1/cyclic AMP-response element-like motif within this region, but not disruption of the adjacent GC-rich/specificity protein-1 sequence, inhibited the inducible promoter activity. Electrophoresis mobility shift assays suggest that the cyclic AMP-response element-binding proteins/activating transcription factor-like factors and Ets-like factors bind constitutively to this bipartite element. Upon induction of differentiation, a shift in the pattern of the cyclic AMP-response element-binding protein/activating transcription factor-like binding factors was observed. Our data indicate that the TfR gene promoter contains an erythroid active element that stimulates the receptor gene transcription upon induction of hemoglobin synthesis.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.subject.meshActivating Transcription Factorsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshBlood Proteins - Metabolismen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Nucleus - Metabolismen_US
dc.subject.meshCyclic Amp - Metabolismen_US
dc.subject.meshCyclic Amp Response Element-Binding Protein - Metabolismen_US
dc.subject.meshErythroid Precursor Cells - Cytology - Metabolismen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshProto-Oncogene Proteins - Metabolismen_US
dc.subject.meshProto-Oncogene Proteins C-Etsen_US
dc.subject.meshReceptors, Estrogen - Metabolismen_US
dc.subject.meshReceptors, Transferrin - Geneticsen_US
dc.subject.meshRegulatory Sequences, Nucleic Aciden_US
dc.subject.meshResponse Elementsen_US
dc.subject.meshTranscription Factors - Metabolismen_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleIdentification of an erythroid active element in the transferrin receptor geneen_US
dc.typeArticleen_US
dc.identifier.emailLok, CN:cnlok@hku.hken_US
dc.identifier.authorityLok, CN=rp00752en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1074/jbc.M000944200en_US
dc.identifier.pmid10811637-
dc.identifier.scopuseid_2-s2.0-0034604603en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034604603&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume275en_US
dc.identifier.issue31en_US
dc.identifier.spage24185en_US
dc.identifier.epage24190en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLok, CN=7006410829en_US
dc.identifier.scopusauthoridPonka, P=7004508750en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats