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Article: Unexpectedly strong binding of a large metal ion (Bi3+) to human serum transferrin

TitleUnexpectedly strong binding of a large metal ion (Bi3+) to human serum transferrin
Authors
Issue Date1996
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 1996, v. 271 n. 16, p. 9483-9489 How to Cite?
AbstractLarge metal ions (>0.9 Å ionic radius) have previously been found to bind only weakly to human serum transferrin (hTF, 80 kDa), presumably because the interdomain cleft cannot close around the metal and synergistic anion. Surprisingly, therefore, we report that Bi3+ (ionic radius 1.03 Å), a metal ion widely used in anti-ulcer drugs, binds strongly to both the N- and C-lobes with log K1* = 19.42 and log K2* = 18.58 (10 mM Hepes, 5 mM bicarbonate, 310 K). The uptake of Bi3+ by apo-hTF from bismuth citrate complexes is very slow (hours), whereas that from bismuth nitrilotriacetate is rapid (minutes). Evidence from absorption and NMR spectroscopy is presented to show that Bi3+ binds to the specific Fe3+ bind-ing sites along with carbonate as the synergistic anion. Under the conditions used, preferential binding of Bi3+to the C-lobe of hTF is observed. Linear free energy relationships show that there is a strong correlation between the strength of binding of Bi3+ and Fe3+ to a wide variety of ligands which include transferrin. Therefore we conclude that the strength of metal ion binding to transferrin is determined more by the ligand donor set than by the size of the ion.
Persistent Identifierhttp://hdl.handle.net/10722/167549
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Hen_US
dc.contributor.authorSadler, PJen_US
dc.contributor.authorSun, Hen_US
dc.date.accessioned2012-10-08T03:08:24Z-
dc.date.available2012-10-08T03:08:24Z-
dc.date.issued1996en_US
dc.identifier.citationJournal Of Biological Chemistry, 1996, v. 271 n. 16, p. 9483-9489en_US
dc.identifier.issn0021-9258en_US
dc.identifier.urihttp://hdl.handle.net/10722/167549-
dc.description.abstractLarge metal ions (>0.9 Å ionic radius) have previously been found to bind only weakly to human serum transferrin (hTF, 80 kDa), presumably because the interdomain cleft cannot close around the metal and synergistic anion. Surprisingly, therefore, we report that Bi3+ (ionic radius 1.03 Å), a metal ion widely used in anti-ulcer drugs, binds strongly to both the N- and C-lobes with log K1* = 19.42 and log K2* = 18.58 (10 mM Hepes, 5 mM bicarbonate, 310 K). The uptake of Bi3+ by apo-hTF from bismuth citrate complexes is very slow (hours), whereas that from bismuth nitrilotriacetate is rapid (minutes). Evidence from absorption and NMR spectroscopy is presented to show that Bi3+ binds to the specific Fe3+ bind-ing sites along with carbonate as the synergistic anion. Under the conditions used, preferential binding of Bi3+to the C-lobe of hTF is observed. Linear free energy relationships show that there is a strong correlation between the strength of binding of Bi3+ and Fe3+ to a wide variety of ligands which include transferrin. Therefore we conclude that the strength of metal ion binding to transferrin is determined more by the ligand donor set than by the size of the ion.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.subject.meshBismuth - Chemistry - Metabolismen_US
dc.subject.meshCationsen_US
dc.subject.meshHumansen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshIron - Metabolismen_US
dc.subject.meshKineticsen_US
dc.subject.meshMagnetic Resonance Spectroscopyen_US
dc.subject.meshMetals - Metabolismen_US
dc.subject.meshModels, Chemicalen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshSpectrophotometry, Ultravioleten_US
dc.subject.meshThermodynamicsen_US
dc.subject.meshTransferrin - Chemistry - Metabolismen_US
dc.titleUnexpectedly strong binding of a large metal ion (Bi3+) to human serum transferrinen_US
dc.typeArticleen_US
dc.identifier.emailSun, H:hsun@hkucc.hku.hken_US
dc.identifier.authoritySun, H=rp00777en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1074/jbc.271.16.9483-
dc.identifier.pmid8621619-
dc.identifier.scopuseid_2-s2.0-0029924479en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029924479&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume271en_US
dc.identifier.issue16en_US
dc.identifier.spage9483en_US
dc.identifier.epage9489en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLi, H=14023043100en_US
dc.identifier.scopusauthoridSadler, PJ=7103024488en_US
dc.identifier.scopusauthoridSun, H=7404827446en_US

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