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Article: Microsomal triglyceride transfer protein (MTP) regulation in HepG2 cells: Insulin negatively regulates MTP gene expression

TitleMicrosomal triglyceride transfer protein (MTP) regulation in HepG2 cells: Insulin negatively regulates MTP gene expression
Authors
Issue Date1995
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jlr.org/
Citation
Journal Of Lipid Research, 1995, v. 36 n. 5, p. 1073-1081 How to Cite?
AbstractThe microsomal triglyceride transfer protein (MTP) is a heterodimeric lipid transfer protein that is required for the assembly and secretion of apoB-containing lipoproteins. In this study, four factors that modulate lipid and lipoprotein metabolism were tested for their ability to regulate MTP levels in HepG2 cells. Of the factors tested, only insulin (≥10-9 M), and high concentrations of glucose (>30 mM) were found to decrease MTP large subunit mRNA levels. Oleate and glucagon had no effect on MTP mRNA levels. The insulin effect was dose- and time-dependent and was mediated through the insulin receptor. In addition, insulin also decreased protein disulfide isomerase (the small subunit of MTP) mRNA levels, although to a lesser extent. Due to the slow turnover rate of MTP (t( 1/2 ) = 44 days), short-term insulin treatment (24 h) did not change MTP activity levels, indicating that the regulation of MTP mRNA levels by insulin is unrelated to insulin's acute inhibition of apoB secretion in HepG2 cells. In summary, MTP mRNA levels are acutely regulated by insulin in HepG2 cells; however, sustained changes in MTP mRNA levels would be required to affect MTP protein levels.
Persistent Identifierhttp://hdl.handle.net/10722/167536
ISSN
2015 Impact Factor: 4.368
2015 SCImago Journal Rankings: 2.529
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLin, MCMen_US
dc.contributor.authorGordon, Den_US
dc.contributor.authorWetterau, JRen_US
dc.date.accessioned2012-10-08T03:08:13Z-
dc.date.available2012-10-08T03:08:13Z-
dc.date.issued1995en_US
dc.identifier.citationJournal Of Lipid Research, 1995, v. 36 n. 5, p. 1073-1081en_US
dc.identifier.issn0022-2275en_US
dc.identifier.urihttp://hdl.handle.net/10722/167536-
dc.description.abstractThe microsomal triglyceride transfer protein (MTP) is a heterodimeric lipid transfer protein that is required for the assembly and secretion of apoB-containing lipoproteins. In this study, four factors that modulate lipid and lipoprotein metabolism were tested for their ability to regulate MTP levels in HepG2 cells. Of the factors tested, only insulin (≥10-9 M), and high concentrations of glucose (>30 mM) were found to decrease MTP large subunit mRNA levels. Oleate and glucagon had no effect on MTP mRNA levels. The insulin effect was dose- and time-dependent and was mediated through the insulin receptor. In addition, insulin also decreased protein disulfide isomerase (the small subunit of MTP) mRNA levels, although to a lesser extent. Due to the slow turnover rate of MTP (t( 1/2 ) = 44 days), short-term insulin treatment (24 h) did not change MTP activity levels, indicating that the regulation of MTP mRNA levels by insulin is unrelated to insulin's acute inhibition of apoB secretion in HepG2 cells. In summary, MTP mRNA levels are acutely regulated by insulin in HepG2 cells; however, sustained changes in MTP mRNA levels would be required to affect MTP protein levels.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jlr.org/en_US
dc.relation.ispartofJournal of Lipid Researchen_US
dc.subject.meshCarrier Proteins - Genetics - Pharmacokineticsen_US
dc.subject.meshCholesterol Ester Transfer Proteinsen_US
dc.subject.meshDepression, Chemicalen_US
dc.subject.meshGene Expression Regulation, Neoplastic - Physiologyen_US
dc.subject.meshGlucose - Pharmacologyen_US
dc.subject.meshGlycoproteinsen_US
dc.subject.meshHalf-Lifeen_US
dc.subject.meshHumansen_US
dc.subject.meshInsulin - Pharmacologyen_US
dc.subject.meshIsomerases - Geneticsen_US
dc.subject.meshPeptide Fragments - Geneticsen_US
dc.subject.meshProtein Disulfide-Isomerasesen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshTriglyceridesen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleMicrosomal triglyceride transfer protein (MTP) regulation in HepG2 cells: Insulin negatively regulates MTP gene expressionen_US
dc.typeArticleen_US
dc.identifier.emailLin, MCM:mcllin@hkucc.hku.hken_US
dc.identifier.authorityLin, MCM=rp00746en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.pmid7658155-
dc.identifier.scopuseid_2-s2.0-0029047046en_US
dc.identifier.volume36en_US
dc.identifier.issue5en_US
dc.identifier.spage1073en_US
dc.identifier.epage1081en_US
dc.identifier.isiWOS:A1995RA51500016-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLin, MCM=7404816359en_US
dc.identifier.scopusauthoridGordon, D=35396397000en_US
dc.identifier.scopusauthoridWetterau, JR=7003768777en_US

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