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Article: Antibody-antigen binding constants determined in solution-phase with the threshold membrane-capture system: Binding constants for anti-fluorescein, anti-saxitoxin, and anti-ricin antibodies

TitleAntibody-antigen binding constants determined in solution-phase with the threshold membrane-capture system: Binding constants for anti-fluorescein, anti-saxitoxin, and anti-ricin antibodies
Authors
Issue Date1994
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yabio
Citation
Analytical Biochemistry, 1994, v. 217 n. 1, p. 128-138 How to Cite?
AbstractAffinities of various monoclonal and polyclonal antibodies for fluorescein-containing antigens, saxitoxin and ricin, were determined by using a light addressable potentiometric sensor-based system (Threshold). The dissociation constants, determined from Scatchard plots, ranged from 2 x 10 -7 to ~3 x 10 -12 M. Dissociation constants for fluorescein and saxitoxin were compared with values determined by independent means. This technique was found to be quick, simple, reproducible, and accurate.
Persistent Identifierhttp://hdl.handle.net/10722/167519
ISSN
2015 Impact Factor: 2.243
2015 SCImago Journal Rankings: 0.725
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDill, Ken_US
dc.contributor.authorLin, Men_US
dc.contributor.authorPoteras, Cen_US
dc.contributor.authorFraser, Cen_US
dc.contributor.authorHafeman, DGen_US
dc.contributor.authorOwicki, JCen_US
dc.contributor.authorOlson, JDen_US
dc.date.accessioned2012-10-08T03:08:00Z-
dc.date.available2012-10-08T03:08:00Z-
dc.date.issued1994en_US
dc.identifier.citationAnalytical Biochemistry, 1994, v. 217 n. 1, p. 128-138en_US
dc.identifier.issn0003-2697en_US
dc.identifier.urihttp://hdl.handle.net/10722/167519-
dc.description.abstractAffinities of various monoclonal and polyclonal antibodies for fluorescein-containing antigens, saxitoxin and ricin, were determined by using a light addressable potentiometric sensor-based system (Threshold). The dissociation constants, determined from Scatchard plots, ranged from 2 x 10 -7 to ~3 x 10 -12 M. Dissociation constants for fluorescein and saxitoxin were compared with values determined by independent means. This technique was found to be quick, simple, reproducible, and accurate.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yabioen_US
dc.relation.ispartofAnalytical Biochemistryen_US
dc.subject.meshAntibodies - Metabolismen_US
dc.subject.meshAntibodies, Monoclonalen_US
dc.subject.meshAntibody Affinityen_US
dc.subject.meshAntigen-Antibody Reactionsen_US
dc.subject.meshBinding, Competitiveen_US
dc.subject.meshFluoresceinen_US
dc.subject.meshFluoresceinsen_US
dc.subject.meshImmunoassay - Methodsen_US
dc.subject.meshKineticsen_US
dc.subject.meshPotentiometry - Methodsen_US
dc.subject.meshRicin - Immunologyen_US
dc.subject.meshSaxitoxin - Immunologyen_US
dc.titleAntibody-antigen binding constants determined in solution-phase with the threshold membrane-capture system: Binding constants for anti-fluorescein, anti-saxitoxin, and anti-ricin antibodiesen_US
dc.typeArticleen_US
dc.identifier.emailLin, M:mcllin@hkucc.hku.hken_US
dc.identifier.authorityLin, M=rp00746en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/abio.1994.1093en_US
dc.identifier.pmid8203727-
dc.identifier.scopuseid_2-s2.0-0028125582en_US
dc.identifier.volume217en_US
dc.identifier.issue1en_US
dc.identifier.spage128en_US
dc.identifier.epage138en_US
dc.identifier.isiWOS:A1994MW32100019-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridDill, K=16434313500en_US
dc.identifier.scopusauthoridLin, M=7404816359en_US
dc.identifier.scopusauthoridPoteras, C=6505706056en_US
dc.identifier.scopusauthoridFraser, C=7401516215en_US
dc.identifier.scopusauthoridHafeman, DG=6603877538en_US
dc.identifier.scopusauthoridOwicki, JC=7003608776en_US
dc.identifier.scopusauthoridOlson, JD=8887451800en_US

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