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Article: Effect of antiserum to a 99 kDa polypeptide on the uptake of taurocholic acid by rat ileal brush border membrane vesicles

TitleEffect of antiserum to a 99 kDa polypeptide on the uptake of taurocholic acid by rat ileal brush border membrane vesicles
Authors
Issue Date1991
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 1991, v. 179 n. 1, p. 204-209 How to Cite?
AbstractA 99 kDa polypeptide in rat ileal brush border membrane (BBM), regarded as a component of the active bile acid transport system on account of photoaffinity labeling, has been purified by affinity chromatography and preparative gel electrophoresis and utilized as an immunogen for raising polyclonal antibody. Immune serum, but not preimmune serum, specifically recognized a single band of 99 kDa protein on immunoblots of ileal and renal BBM. In contrast, no reactivity was observed with proteins in jejunal BBM. This polyclonal antibody, compared with preimmune serum and anticytosolic bile acid binding protein (14 kDa) serum, significantly inhibited the Na + dependent uptake of [ 3H] taurocholate by BBM vesicles (p < 0.01). [ 14C]D-glucose uptake by BBM vesicles was not influenced by the immune serum (p < 0.01). Thus, these studies provide further support for the specific role of a 99 kDa protein in ileal BBM bile acid transport.
Persistent Identifierhttp://hdl.handle.net/10722/167500
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGong, YZen_US
dc.contributor.authorZwarych Jr, PPen_US
dc.contributor.authorLin, MCen_US
dc.contributor.authorWilson, FAen_US
dc.date.accessioned2012-10-08T03:07:46Z-
dc.date.available2012-10-08T03:07:46Z-
dc.date.issued1991en_US
dc.identifier.citationBiochemical And Biophysical Research Communications, 1991, v. 179 n. 1, p. 204-209en_US
dc.identifier.issn0006-291Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/167500-
dc.description.abstractA 99 kDa polypeptide in rat ileal brush border membrane (BBM), regarded as a component of the active bile acid transport system on account of photoaffinity labeling, has been purified by affinity chromatography and preparative gel electrophoresis and utilized as an immunogen for raising polyclonal antibody. Immune serum, but not preimmune serum, specifically recognized a single band of 99 kDa protein on immunoblots of ileal and renal BBM. In contrast, no reactivity was observed with proteins in jejunal BBM. This polyclonal antibody, compared with preimmune serum and anticytosolic bile acid binding protein (14 kDa) serum, significantly inhibited the Na + dependent uptake of [ 3H] taurocholate by BBM vesicles (p < 0.01). [ 14C]D-glucose uptake by BBM vesicles was not influenced by the immune serum (p < 0.01). Thus, these studies provide further support for the specific role of a 99 kDa protein in ileal BBM bile acid transport.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_US
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodiesen_US
dc.subject.meshBiological Transporten_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshGlucose - Metabolismen_US
dc.subject.meshIleum - Metabolismen_US
dc.subject.meshImmunoblottingen_US
dc.subject.meshJejunum - Metabolismen_US
dc.subject.meshKidney - Metabolismen_US
dc.subject.meshKineticsen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Proteins - Immunology - Isolation & Purification - Metabolismen_US
dc.subject.meshMicrovilli - Metabolismen_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Strainsen_US
dc.subject.meshTaurocholic Acid - Metabolismen_US
dc.titleEffect of antiserum to a 99 kDa polypeptide on the uptake of taurocholic acid by rat ileal brush border membrane vesiclesen_US
dc.typeArticleen_US
dc.identifier.emailLin, MC:mcllin@hkucc.hku.hken_US
dc.identifier.authorityLin, MC=rp00746en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0006-291X(91)91355-G-
dc.identifier.pmid1883352-
dc.identifier.scopuseid_2-s2.0-0026055970en_US
dc.identifier.volume179en_US
dc.identifier.issue1en_US
dc.identifier.spage204en_US
dc.identifier.epage209en_US
dc.identifier.isiWOS:A1991GD09200031-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridGong, YZ=7402475196en_US
dc.identifier.scopusauthoridZwarych Jr, PP=8871203900en_US
dc.identifier.scopusauthoridLin, MC=7404816359en_US
dc.identifier.scopusauthoridWilson, FA=7202849433en_US

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