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Article: Induction of glucagon sensitivity in a transformed kidney cell line by prostaglandin E2 and its inhibition by epidermal growth factor.

TitleInduction of glucagon sensitivity in a transformed kidney cell line by prostaglandin E2 and its inhibition by epidermal growth factor.
Authors
Issue Date1987
Citation
Molecular And Cellular Biology, 1987, v. 7 n. 12, p. 4324-4328 How to Cite?
AbstractA model system using a transformed dog kidney cell line (Madin-Darby canine kidney), has been established for studying the process of differentiation. Glucagon responsiveness can be restored to these transformed cells by various differentiation inducers, including prostaglandin E2. Glucagon response was measured in terms of the ability of glucagon to stimulate cAMP production. Induction of glucagon sensitivity seems to be mediated by cAMP. The ability of various prostaglandin analogs to elevate the cAMP level correlates closely with their ability to induce glucagon sensitivity. In fact, 8-Br-cAMP is also a potent inducer. To define the nature of this cAMP-mediated process, we identified several inhibitors of this induction process. These differentiation inhibitors include serum, phorbol ester, and epidermal growth factor. These inhibitors do not have a direct effect on cAMP production by cells in the presence or absence of hormones. Furthermore, induction by 8-Br-cAMP is also inhibited by these agents. Therefore, the site of inhibition is located beyond the point of cAMP production. Possible interaction between cAMP- and epidermal growth factor-dependent phosphorylations is discussed.
Persistent Identifierhttp://hdl.handle.net/10722/167475
ISSN
2015 Impact Factor: 4.427
2015 SCImago Journal Rankings: 3.806
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLin, MCen_US
dc.contributor.authorDarfler, FJen_US
dc.contributor.authorBeckner, SKen_US
dc.date.accessioned2012-10-08T03:07:28Z-
dc.date.available2012-10-08T03:07:28Z-
dc.date.issued1987en_US
dc.identifier.citationMolecular And Cellular Biology, 1987, v. 7 n. 12, p. 4324-4328en_US
dc.identifier.issn0270-7306en_US
dc.identifier.urihttp://hdl.handle.net/10722/167475-
dc.description.abstractA model system using a transformed dog kidney cell line (Madin-Darby canine kidney), has been established for studying the process of differentiation. Glucagon responsiveness can be restored to these transformed cells by various differentiation inducers, including prostaglandin E2. Glucagon response was measured in terms of the ability of glucagon to stimulate cAMP production. Induction of glucagon sensitivity seems to be mediated by cAMP. The ability of various prostaglandin analogs to elevate the cAMP level correlates closely with their ability to induce glucagon sensitivity. In fact, 8-Br-cAMP is also a potent inducer. To define the nature of this cAMP-mediated process, we identified several inhibitors of this induction process. These differentiation inhibitors include serum, phorbol ester, and epidermal growth factor. These inhibitors do not have a direct effect on cAMP production by cells in the presence or absence of hormones. Furthermore, induction by 8-Br-cAMP is also inhibited by these agents. Therefore, the site of inhibition is located beyond the point of cAMP production. Possible interaction between cAMP- and epidermal growth factor-dependent phosphorylations is discussed.en_US
dc.languageengen_US
dc.relation.ispartofMolecular and Cellular Biologyen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.mesh8-Bromo Cyclic Adenosine Monophosphate - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlooden_US
dc.subject.meshCell Differentiation - Drug Effectsen_US
dc.subject.meshCell Line, Transformeden_US
dc.subject.meshCyclic Amp - Biosynthesisen_US
dc.subject.meshDinoprostoneen_US
dc.subject.meshDogsen_US
dc.subject.meshEpidermal Growth Factor - Pharmacologyen_US
dc.subject.meshGlucagon - Pharmacologyen_US
dc.subject.meshKidneyen_US
dc.subject.meshProstaglandins - Pharmacologyen_US
dc.subject.meshProstaglandins E - Pharmacologyen_US
dc.subject.meshTetradecanoylphorbol Acetate - Pharmacologyen_US
dc.titleInduction of glucagon sensitivity in a transformed kidney cell line by prostaglandin E2 and its inhibition by epidermal growth factor.en_US
dc.typeArticleen_US
dc.identifier.emailLin, MC:mcllin@hkucc.hku.hken_US
dc.identifier.authorityLin, MC=rp00746en_US
dc.description.naturepublished_or_final_versionen_US
dc.identifier.pmid2830489-
dc.identifier.pmcidPMC368115-
dc.identifier.scopuseid_2-s2.0-0023461443en_US
dc.identifier.volume7en_US
dc.identifier.issue12en_US
dc.identifier.spage4324en_US
dc.identifier.epage4328en_US
dc.identifier.isiWOS:A1987K958600021-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLin, MC=7404816359en_US
dc.identifier.scopusauthoridDarfler, FJ=6603120994en_US
dc.identifier.scopusauthoridBeckner, SK=7004064238en_US

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