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Article: Revertants of Ha-MuSV-transformed MDCK cells express reduced levels of p21 and possess a more normal phenotype

TitleRevertants of Ha-MuSV-transformed MDCK cells express reduced levels of p21 and possess a more normal phenotype
Authors
Issue Date1986
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexcr
Citation
Experimental Cell Research, 1986, v. 162 n. 2, p. 335-346 How to Cite?
AbstractFour subclones of the originally cloned Harvey murine sarcoma virus-transformed Madin Darby canine kidney (MDCK) cells have been isolated. These subclones fall into two general classes. Two subclones have a fibroblastic morphology, have lost the growth requirement for prostaglandin E 1 (PGE 1), do not respond to glucagon or vasopressin, and, in general, appear transformed. Two other subclones have epithelioid morphologies, are growth-stimulated by PGE 1, respond to vasopressin with an increase in intracellular cAMP. We propose that these cells represent revertants to a more non-transformed phenotype. Unlike normal cells, however, these revertants grow under anchorage-independent conditions, express detectable but reduceced amounts of the transforming gene product, p21, and grow in nude mice. The appearance of such revertants may be one cause of the observed heterogeneity of tumor cells.
Persistent Identifierhttp://hdl.handle.net/10722/167470
ISSN
2015 Impact Factor: 3.378
2015 SCImago Journal Rankings: 1.900
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDarfler, FJen_US
dc.contributor.authorShih, TYen_US
dc.contributor.authorLin, MCen_US
dc.date.accessioned2012-10-08T03:07:24Z-
dc.date.available2012-10-08T03:07:24Z-
dc.date.issued1986en_US
dc.identifier.citationExperimental Cell Research, 1986, v. 162 n. 2, p. 335-346en_US
dc.identifier.issn0014-4827en_US
dc.identifier.urihttp://hdl.handle.net/10722/167470-
dc.description.abstractFour subclones of the originally cloned Harvey murine sarcoma virus-transformed Madin Darby canine kidney (MDCK) cells have been isolated. These subclones fall into two general classes. Two subclones have a fibroblastic morphology, have lost the growth requirement for prostaglandin E 1 (PGE 1), do not respond to glucagon or vasopressin, and, in general, appear transformed. Two other subclones have epithelioid morphologies, are growth-stimulated by PGE 1, respond to vasopressin with an increase in intracellular cAMP. We propose that these cells represent revertants to a more non-transformed phenotype. Unlike normal cells, however, these revertants grow under anchorage-independent conditions, express detectable but reduceced amounts of the transforming gene product, p21, and grow in nude mice. The appearance of such revertants may be one cause of the observed heterogeneity of tumor cells.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexcren_US
dc.relation.ispartofExperimental Cell Researchen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Division - Drug Effectsen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Transformation, Viralen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshCyclic Amp - Metabolismen_US
dc.subject.meshDinoprostoneen_US
dc.subject.meshDogsen_US
dc.subject.meshGlucagon - Pharmacologyen_US
dc.subject.meshGrowth Substancesen_US
dc.subject.meshHarvey Murine Sarcoma Virus - Geneticsen_US
dc.subject.meshMutationen_US
dc.subject.meshOncogene Proteins, Viral - Genetics - Metabolismen_US
dc.subject.meshProstaglandins E - Metabolismen_US
dc.subject.meshSarcoma, Experimental - Genetics - Microbiology - Pathologyen_US
dc.subject.meshVasopressins - Pharmacologyen_US
dc.titleRevertants of Ha-MuSV-transformed MDCK cells express reduced levels of p21 and possess a more normal phenotypeen_US
dc.typeArticleen_US
dc.identifier.emailLin, MC:mcllin@hkucc.hku.hken_US
dc.identifier.authorityLin, MC=rp00746en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0014-4827(86)90339-3-
dc.identifier.pmid3002821-
dc.identifier.scopuseid_2-s2.0-0022590855en_US
dc.identifier.volume162en_US
dc.identifier.issue2en_US
dc.identifier.spage335en_US
dc.identifier.epage346en_US
dc.identifier.isiWOS:A1986AYY0600005-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridDarfler, FJ=6603120994en_US
dc.identifier.scopusauthoridShih, TY=7202664631en_US
dc.identifier.scopusauthoridLin, MC=7404816359en_US

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