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Article: Expression of glucagon sensitivity by transformed MDCK cells normally unresponsive to glucagon: Early commitment to differentiation

TitleExpression of glucagon sensitivity by transformed MDCK cells normally unresponsive to glucagon: Early commitment to differentiation
Authors
Issue Date1985
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010
Citation
Journal Of Cellular Physiology, 1985, v. 125 n. 2, p. 295-300 How to Cite?
AbstractA cloned line of canine kidney cells (MDCK) transformed with Harvey murine sarcoma virus, in contrast to the parental, untransformed line, expressed glucagon sensitivity only under controlled culture conditions. The glucagon sensitivity of transformed MDCK cells appeared after 10 days of culture if plated at less than 100,000 cells/dish or after 3 days if cells were plated at greater than 300,000 cells/dish. As there was no effect of conditioned medium from glucagon-sensitive cells on insensitive cells, media components seemed not be involved in this phenomenon. Glucagon sensitivity appeared more readily in defined as opposed to serum-containing medium. In fact, as little as 2% fetal bovine serum inhibited the expression of glucagon sensitivity when included in defined medium over the course of the experiment. Furthermore, when transformed MDCK cells were exposed to serum for only the first 24 hr of culture, glucagon sensitivity on day 11 was identical to that of cells exposed to serum throughout the entire experiment. In contrast, exposure to serum later in culture (days 4-8) had no inhibitory effect on the expression of glucagon sensitivity on day 11. The data suggest that differentiation, or glucagon sensitivity, occurs when transformed, glucagon-insensitive cells achieve a critical high density and that differentiation is sensitive to inhibition by serum only during the first 24 hr of culture.
Persistent Identifierhttp://hdl.handle.net/10722/167469
ISSN
2015 Impact Factor: 4.155
2015 SCImago Journal Rankings: 1.842
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBeckner, SKen_US
dc.contributor.authorLin, MCen_US
dc.date.accessioned2012-10-08T03:07:24Z-
dc.date.available2012-10-08T03:07:24Z-
dc.date.issued1985en_US
dc.identifier.citationJournal Of Cellular Physiology, 1985, v. 125 n. 2, p. 295-300en_US
dc.identifier.issn0021-9541en_US
dc.identifier.urihttp://hdl.handle.net/10722/167469-
dc.description.abstractA cloned line of canine kidney cells (MDCK) transformed with Harvey murine sarcoma virus, in contrast to the parental, untransformed line, expressed glucagon sensitivity only under controlled culture conditions. The glucagon sensitivity of transformed MDCK cells appeared after 10 days of culture if plated at less than 100,000 cells/dish or after 3 days if cells were plated at greater than 300,000 cells/dish. As there was no effect of conditioned medium from glucagon-sensitive cells on insensitive cells, media components seemed not be involved in this phenomenon. Glucagon sensitivity appeared more readily in defined as opposed to serum-containing medium. In fact, as little as 2% fetal bovine serum inhibited the expression of glucagon sensitivity when included in defined medium over the course of the experiment. Furthermore, when transformed MDCK cells were exposed to serum for only the first 24 hr of culture, glucagon sensitivity on day 11 was identical to that of cells exposed to serum throughout the entire experiment. In contrast, exposure to serum later in culture (days 4-8) had no inhibitory effect on the expression of glucagon sensitivity on day 11. The data suggest that differentiation, or glucagon sensitivity, occurs when transformed, glucagon-insensitive cells achieve a critical high density and that differentiation is sensitive to inhibition by serum only during the first 24 hr of culture.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010en_US
dc.relation.ispartofJournal of Cellular Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlooden_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Transformation, Viralen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshDogsen_US
dc.subject.meshDrug Resistanceen_US
dc.subject.meshGlucagon - Antagonists & Inhibitors - Pharmacologyen_US
dc.subject.meshKidney - Cytology - Drug Effectsen_US
dc.subject.meshTime Factorsen_US
dc.titleExpression of glucagon sensitivity by transformed MDCK cells normally unresponsive to glucagon: Early commitment to differentiationen_US
dc.typeArticleen_US
dc.identifier.emailLin, MC:mcllin@hkucc.hku.hken_US
dc.identifier.authorityLin, MC=rp00746en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/jcp.1041250218-
dc.identifier.pmid4055913-
dc.identifier.scopuseid_2-s2.0-0022375939en_US
dc.identifier.volume125en_US
dc.identifier.issue2en_US
dc.identifier.spage295en_US
dc.identifier.epage300en_US
dc.identifier.isiWOS:A1985ASW7000017-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridBeckner, SK=7004064238en_US
dc.identifier.scopusauthoridLin, MC=7404816359en_US

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