File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: [31] Characterization of hormone-sensitive Madin-Darby canine kidney cells

Title[31] Characterization of hormone-sensitive Madin-Darby canine kidney cells
Other TitlesCharacterization of hormone-sensitive Madin-Darby canine kidney cells
Authors
Issue Date1985
PublisherAcademic Press. The Journal's web site is located at http://www.sciencedirect.com/science/bookseries/00766879
Citation
Methods In Enzymology, 1985, v. 109, p. 360-365 How to Cite?
AbstractCultured cells, especially the established lines, provide a continuous and homogeneous system for studying hormone action in intact cells. The cell line known as Madin–Darby canine kidney (MDCK) cells, derived from normal dog kidney more than 20 years ago, is ideal for this type of research, because it retains differentiated functions in culture and, in addition, responds to several hormones, including glucagon, vasopressin, β-adrenergic agonists, and prostaglandins. This chapter deals mainly with the optimal culture conditions for maintaining hormone responsiveness, the measurement of intracellular cyclic adenosine monophosphate (AMP). The chapter also discusses the characteristics of several types of hormone sensitivity in MDCK cells and concludes that MDCK cells have been used as a model system for studying hormone regulation of kidney functions. The availability of a differentiated, hormone-responsive cell line facilitates the studies of the cascade of biochemical events subsequent to cyclic AMP elevation and its correlation with specific kidney functions.
Persistent Identifierhttp://hdl.handle.net/10722/167464
ISSN
2014 Impact Factor: 2.088
2015 SCImago Journal Rankings: 1.501

 

DC FieldValueLanguage
dc.contributor.authorLin, MCen_US
dc.contributor.authorBeckner, SKen_US
dc.contributor.authorDarfler, FJen_US
dc.date.accessioned2012-10-08T03:07:20Z-
dc.date.available2012-10-08T03:07:20Z-
dc.date.issued1985en_US
dc.identifier.citationMethods In Enzymology, 1985, v. 109, p. 360-365en_US
dc.identifier.issn0076-6879en_US
dc.identifier.urihttp://hdl.handle.net/10722/167464-
dc.description.abstractCultured cells, especially the established lines, provide a continuous and homogeneous system for studying hormone action in intact cells. The cell line known as Madin–Darby canine kidney (MDCK) cells, derived from normal dog kidney more than 20 years ago, is ideal for this type of research, because it retains differentiated functions in culture and, in addition, responds to several hormones, including glucagon, vasopressin, β-adrenergic agonists, and prostaglandins. This chapter deals mainly with the optimal culture conditions for maintaining hormone responsiveness, the measurement of intracellular cyclic adenosine monophosphate (AMP). The chapter also discusses the characteristics of several types of hormone sensitivity in MDCK cells and concludes that MDCK cells have been used as a model system for studying hormone regulation of kidney functions. The availability of a differentiated, hormone-responsive cell line facilitates the studies of the cascade of biochemical events subsequent to cyclic AMP elevation and its correlation with specific kidney functions.-
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.sciencedirect.com/science/bookseries/00766879en_US
dc.relation.ispartofMethods in Enzymologyen_US
dc.title[31] Characterization of hormone-sensitive Madin-Darby canine kidney cellsen_US
dc.title.alternativeCharacterization of hormone-sensitive Madin-Darby canine kidney cells-
dc.typeArticleen_US
dc.identifier.emailLin, MC:mcllin@hkucc.hku.hken_US
dc.identifier.authorityLin, MC=rp00746en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0076-6879(85)09101-7-
dc.identifier.scopuseid_2-s2.0-0021893844en_US
dc.identifier.volume109en_US
dc.identifier.spage360en_US
dc.identifier.epage365en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLin, MC=7404816359en_US
dc.identifier.scopusauthoridBeckner, SK=7004064238en_US
dc.identifier.scopusauthoridDarfler, FJ=6603120994en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats