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Article: Preparation and characterization of pentaammineruthenium-(histidine-83)azurin: thermodynamics of intramolecular electron transfer from ruthenium to copper.

TitlePreparation and characterization of pentaammineruthenium-(histidine-83)azurin: thermodynamics of intramolecular electron transfer from ruthenium to copper.
Authors
Issue Date1984
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
Proceedings Of The National Academy Of Sciences Of The United States Of America, 1984, v. 81 n. 20, p. 6554-6558 How to Cite?
AbstractThe reaction between a5RuH2O2+ (a is NH3) and Pseudomonas aeruginosa azurin at pH 7, followed by oxidation, yields a5Ru(His-83)3+-azurin(Cu2+) as the major product. Spectroscopic measurements (UV-visible, CD, EPR, and resonance Raman) indicate that the native structure is maintained in the modified protein. The site of ruthenium binding (His-83) was identified by peptide mapping. The a5RuHis/Cu ratio in the modified protein, determined from the EPR spectrum, is 1:1, and the reduction potentials (vs. normal hydrogen electrode, pH 7.0, 25 degrees C) are blue copper (Cu2+/1+), 320 +/- 2 mV; a5Ru(His-83)3+/2+, 50 +/- 10 mV. From measurements of the reduction potentials at several temperatures in the 5-40 degrees C range, delta H degree for intramolecular Ru2+ ----Cu2+ electron transfer was estimated to be -12.4 kcal mol-1 (1 cal = 4.184 J). Analysis of kinetic data in light of the electron transfer exothermicity indicates that the reorganizational enthalpy of the blue copper site can be no larger than 7.1 kcal mol-1.
Persistent Identifierhttp://hdl.handle.net/10722/167461
ISSN
2015 Impact Factor: 9.423
2015 SCImago Journal Rankings: 6.883
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMargalit, Ren_US
dc.contributor.authorKostić, NMen_US
dc.contributor.authorChe, CMen_US
dc.contributor.authorBlair, DFen_US
dc.contributor.authorChiang, HJen_US
dc.contributor.authorPecht, Ien_US
dc.contributor.authorShelton, JBen_US
dc.contributor.authorShelton, JRen_US
dc.contributor.authorSchroeder, WAen_US
dc.contributor.authorGray, HBen_US
dc.date.accessioned2012-10-08T03:07:19Z-
dc.date.available2012-10-08T03:07:19Z-
dc.date.issued1984en_US
dc.identifier.citationProceedings Of The National Academy Of Sciences Of The United States Of America, 1984, v. 81 n. 20, p. 6554-6558en_US
dc.identifier.issn0027-8424en_US
dc.identifier.urihttp://hdl.handle.net/10722/167461-
dc.description.abstractThe reaction between a5RuH2O2+ (a is NH3) and Pseudomonas aeruginosa azurin at pH 7, followed by oxidation, yields a5Ru(His-83)3+-azurin(Cu2+) as the major product. Spectroscopic measurements (UV-visible, CD, EPR, and resonance Raman) indicate that the native structure is maintained in the modified protein. The site of ruthenium binding (His-83) was identified by peptide mapping. The a5RuHis/Cu ratio in the modified protein, determined from the EPR spectrum, is 1:1, and the reduction potentials (vs. normal hydrogen electrode, pH 7.0, 25 degrees C) are blue copper (Cu2+/1+), 320 +/- 2 mV; a5Ru(His-83)3+/2+, 50 +/- 10 mV. From measurements of the reduction potentials at several temperatures in the 5-40 degrees C range, delta H degree for intramolecular Ru2+ ----Cu2+ electron transfer was estimated to be -12.4 kcal mol-1 (1 cal = 4.184 J). Analysis of kinetic data in light of the electron transfer exothermicity indicates that the reorganizational enthalpy of the blue copper site can be no larger than 7.1 kcal mol-1.en_US
dc.languageengen_US
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.orgen_US
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen_US
dc.subject.meshAzurinen_US
dc.subject.meshBacterial Proteinsen_US
dc.subject.meshCopperen_US
dc.subject.meshOrganometallic Compoundsen_US
dc.subject.meshOxidation-Reductionen_US
dc.subject.meshRutheniumen_US
dc.subject.meshThermodynamicsen_US
dc.titlePreparation and characterization of pentaammineruthenium-(histidine-83)azurin: thermodynamics of intramolecular electron transfer from ruthenium to copper.en_US
dc.typeArticleen_US
dc.identifier.emailChe, CM:cmche@hku.hken_US
dc.identifier.authorityChe, CM=rp00670en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1073/pnas.81.20.6554-
dc.identifier.pmid6593716-
dc.identifier.scopuseid_2-s2.0-0021505091en_US
dc.identifier.volume81en_US
dc.identifier.issue20en_US
dc.identifier.spage6554en_US
dc.identifier.epage6558en_US
dc.identifier.isiWOS:A1984TS38500002-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridMargalit, R=7005792437en_US
dc.identifier.scopusauthoridKostić, NM=7005929778en_US
dc.identifier.scopusauthoridChe, CM=7102442791en_US
dc.identifier.scopusauthoridBlair, DF=7202956822en_US
dc.identifier.scopusauthoridChiang, HJ=7402271927en_US
dc.identifier.scopusauthoridPecht, I=7102376462en_US
dc.identifier.scopusauthoridShelton, JB=7202118768en_US
dc.identifier.scopusauthoridShelton, JR=35564886700en_US
dc.identifier.scopusauthoridSchroeder, WA=7201899977en_US
dc.identifier.scopusauthoridGray, HB=36047602600en_US

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