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Article: Changes in adenylate cyclase and phosphodiesterase activities during the growth cycle of adult rat hepatocytes in primary culture

TitleChanges in adenylate cyclase and phosphodiesterase activities during the growth cycle of adult rat hepatocytes in primary culture
Authors
Issue Date1984
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yabbi
Citation
Archives Of Biochemistry And Biophysics, 1984, v. 232 n. 2, p. 679-684 How to Cite?
AbstractLong-term primary adult rat hepatocyte cultures show growth-state-dependent changes in adenylate cyclase and cAMP phosphodiesterase activities. Cellular adenylate cyclase activity decreases to undetectable levels within 1 day postplating, reappears on Days 4–5, and becomes maximal on Day 9. Membrane adenylate cyclase and cellular cAMP formation are insensitive to glucagon during log phase (Days 4–8) but not during lag (Day 1) or stationary phase (Day 12). Cyclic AMP phosphodiesterase activities (soluble and particulate) fall ∼-70% by Day 2 but recover as proliferation begins. By contrast, the particulate phosphodiesterase assayed at 100 μm cAMP, decreased during Days 0–2. These observations simulate changes seen during liver proliferative transitions in vivo and, therefore, further support the use of these cultures as a developmental model.
Persistent Identifierhttp://hdl.handle.net/10722/167458
ISSN
2015 Impact Factor: 2.807
2015 SCImago Journal Rankings: 1.478
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLad, PJen_US
dc.contributor.authorBlazquez, Een_US
dc.contributor.authorLin, Men_US
dc.contributor.authorLeffert, HLen_US
dc.date.accessioned2012-10-08T03:07:16Z-
dc.date.available2012-10-08T03:07:16Z-
dc.date.issued1984en_US
dc.identifier.citationArchives Of Biochemistry And Biophysics, 1984, v. 232 n. 2, p. 679-684en_US
dc.identifier.issn0003-9861en_US
dc.identifier.urihttp://hdl.handle.net/10722/167458-
dc.description.abstractLong-term primary adult rat hepatocyte cultures show growth-state-dependent changes in adenylate cyclase and cAMP phosphodiesterase activities. Cellular adenylate cyclase activity decreases to undetectable levels within 1 day postplating, reappears on Days 4–5, and becomes maximal on Day 9. Membrane adenylate cyclase and cellular cAMP formation are insensitive to glucagon during log phase (Days 4–8) but not during lag (Day 1) or stationary phase (Day 12). Cyclic AMP phosphodiesterase activities (soluble and particulate) fall ∼-70% by Day 2 but recover as proliferation begins. By contrast, the particulate phosphodiesterase assayed at 100 μm cAMP, decreased during Days 0–2. These observations simulate changes seen during liver proliferative transitions in vivo and, therefore, further support the use of these cultures as a developmental model.-
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yabbien_US
dc.relation.ispartofArchives of Biochemistry and Biophysicsen_US
dc.subject.mesh3',5'-Cyclic-Amp Phosphodiesterases - Metabolismen_US
dc.subject.meshAdenylate Cyclase - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Cycleen_US
dc.subject.meshCell Divisionen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshGlucagon - Pharmacologyen_US
dc.subject.meshLiver - Cytology - Enzymologyen_US
dc.subject.meshMaleen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred F344en_US
dc.titleChanges in adenylate cyclase and phosphodiesterase activities during the growth cycle of adult rat hepatocytes in primary cultureen_US
dc.typeArticleen_US
dc.identifier.emailLin, M:mcllin@hkucc.hku.hken_US
dc.identifier.authorityLin, M=rp00746en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0003-9861(84)90588-5-
dc.identifier.pmid6087736-
dc.identifier.scopuseid_2-s2.0-0021234034en_US
dc.identifier.volume232en_US
dc.identifier.issue2en_US
dc.identifier.spage679en_US
dc.identifier.epage684en_US
dc.identifier.isiWOS:A1984TB63200030-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLad, PJ=7006446790en_US
dc.identifier.scopusauthoridBlazquez, E=7006079804en_US
dc.identifier.scopusauthoridLin, M=7404816359en_US
dc.identifier.scopusauthoridLeffert, HL=7005359834en_US

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