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- Publisher Website: 10.1080/10826068408070618
- Scopus: eid_2-s2.0-0021228820
- PMID: 6091090
- WOS: WOS:A1984TB60000002
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Article: Purification of the photoaffinity-labeled glucagon receptor by gel electrophoretic methods
Title | Purification of the photoaffinity-labeled glucagon receptor by gel electrophoretic methods |
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Authors | |
Issue Date | 1984 |
Citation | Preparative Biochemistry, 1984, v. 14 n. 2, p. 99-121 How to Cite? |
Abstract | Rat liver plasma membrane glucagon receptor has been purified with a yield of 0.01% to an estimated homogeneity of 32-60%, using a 2-stage electrophoretic procedure. SDS-solubilized membrane proteins labeled by the photoaffinity-agent, Ne-4-azidophenylamidinoglucagon (APA-glucagon), were separated by polyacrylamide gel electrophoresis in SDS-containing buffers. Gel slices corresponding to the molecular weight of the receptor were excised, electrophoretically extracted and concentrated. The concentrate was subjected to isoelectric focusing on Sephadex to yield a purified product in which the photoaffinity-labeled receptor, with a molecular weight of 56K and a pI' of 5.9, is the sole major component. |
Persistent Identifier | http://hdl.handle.net/10722/167457 |
ISSN | |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Horuk, R | en_US |
dc.contributor.author | Beckner, S | en_US |
dc.contributor.author | Lin, M | en_US |
dc.date.accessioned | 2012-10-08T03:07:13Z | - |
dc.date.available | 2012-10-08T03:07:13Z | - |
dc.date.issued | 1984 | en_US |
dc.identifier.citation | Preparative Biochemistry, 1984, v. 14 n. 2, p. 99-121 | en_US |
dc.identifier.issn | 0032-7484 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/167457 | - |
dc.description.abstract | Rat liver plasma membrane glucagon receptor has been purified with a yield of 0.01% to an estimated homogeneity of 32-60%, using a 2-stage electrophoretic procedure. SDS-solubilized membrane proteins labeled by the photoaffinity-agent, Ne-4-azidophenylamidinoglucagon (APA-glucagon), were separated by polyacrylamide gel electrophoresis in SDS-containing buffers. Gel slices corresponding to the molecular weight of the receptor were excised, electrophoretically extracted and concentrated. The concentrate was subjected to isoelectric focusing on Sephadex to yield a purified product in which the photoaffinity-labeled receptor, with a molecular weight of 56K and a pI' of 5.9, is the sole major component. | - |
dc.language | eng | en_US |
dc.relation.ispartof | Preparative Biochemistry | en_US |
dc.subject.mesh | Affinity Labels | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Azides | en_US |
dc.subject.mesh | Cell Membrane - Metabolism | en_US |
dc.subject.mesh | Electrophoresis, Polyacrylamide Gel - Methods | en_US |
dc.subject.mesh | Glucagon - Analogs & Derivatives - Metabolism | en_US |
dc.subject.mesh | Isoelectric Focusing | en_US |
dc.subject.mesh | Liver - Metabolism | en_US |
dc.subject.mesh | Molecular Weight | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Receptors, Cell Surface - Isolation & Purification - Metabolism | en_US |
dc.subject.mesh | Receptors, Glucagon | en_US |
dc.title | Purification of the photoaffinity-labeled glucagon receptor by gel electrophoretic methods | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lin, M:mcllin@hkucc.hku.hk | en_US |
dc.identifier.authority | Lin, M=rp00746 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1080/10826068408070618 | - |
dc.identifier.pmid | 6091090 | - |
dc.identifier.scopus | eid_2-s2.0-0021228820 | en_US |
dc.identifier.volume | 14 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 99 | en_US |
dc.identifier.epage | 121 | en_US |
dc.identifier.isi | WOS:A1984TB60000002 | - |
dc.identifier.scopusauthorid | Horuk, R=7007108105 | en_US |
dc.identifier.scopusauthorid | Beckner, S=7004064238 | en_US |
dc.identifier.scopusauthorid | Lin, M=7404816359 | en_US |
dc.identifier.issnl | 0032-7484 | - |