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Article: Purification of the photoaffinity-labeled glucagon receptor by gel electrophoretic methods

TitlePurification of the photoaffinity-labeled glucagon receptor by gel electrophoretic methods
Authors
Issue Date1984
Citation
Preparative Biochemistry, 1984, v. 14 n. 2, p. 99-121 How to Cite?
AbstractRat liver plasma membrane glucagon receptor has been purified with a yield of 0.01% to an estimated homogeneity of 32-60%, using a 2-stage electrophoretic procedure. SDS-solubilized membrane proteins labeled by the photoaffinity-agent, Ne-4-azidophenylamidinoglucagon (APA-glucagon), were separated by polyacrylamide gel electrophoresis in SDS-containing buffers. Gel slices corresponding to the molecular weight of the receptor were excised, electrophoretically extracted and concentrated. The concentrate was subjected to isoelectric focusing on Sephadex to yield a purified product in which the photoaffinity-labeled receptor, with a molecular weight of 56K and a pI' of 5.9, is the sole major component.
Persistent Identifierhttp://hdl.handle.net/10722/167457
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHoruk, Ren_US
dc.contributor.authorBeckner, Sen_US
dc.contributor.authorLin, Men_US
dc.date.accessioned2012-10-08T03:07:13Z-
dc.date.available2012-10-08T03:07:13Z-
dc.date.issued1984en_US
dc.identifier.citationPreparative Biochemistry, 1984, v. 14 n. 2, p. 99-121en_US
dc.identifier.issn0032-7484en_US
dc.identifier.urihttp://hdl.handle.net/10722/167457-
dc.description.abstractRat liver plasma membrane glucagon receptor has been purified with a yield of 0.01% to an estimated homogeneity of 32-60%, using a 2-stage electrophoretic procedure. SDS-solubilized membrane proteins labeled by the photoaffinity-agent, Ne-4-azidophenylamidinoglucagon (APA-glucagon), were separated by polyacrylamide gel electrophoresis in SDS-containing buffers. Gel slices corresponding to the molecular weight of the receptor were excised, electrophoretically extracted and concentrated. The concentrate was subjected to isoelectric focusing on Sephadex to yield a purified product in which the photoaffinity-labeled receptor, with a molecular weight of 56K and a pI' of 5.9, is the sole major component.-
dc.languageengen_US
dc.relation.ispartofPreparative Biochemistryen_US
dc.subject.meshAffinity Labelsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAzidesen_US
dc.subject.meshCell Membrane - Metabolismen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gel - Methodsen_US
dc.subject.meshGlucagon - Analogs & Derivatives - Metabolismen_US
dc.subject.meshIsoelectric Focusingen_US
dc.subject.meshLiver - Metabolismen_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshRatsen_US
dc.subject.meshReceptors, Cell Surface - Isolation & Purification - Metabolismen_US
dc.subject.meshReceptors, Glucagonen_US
dc.titlePurification of the photoaffinity-labeled glucagon receptor by gel electrophoretic methodsen_US
dc.typeArticleen_US
dc.identifier.emailLin, M:mcllin@hkucc.hku.hken_US
dc.identifier.authorityLin, M=rp00746en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1080/10826068408070618-
dc.identifier.pmid6091090-
dc.identifier.scopuseid_2-s2.0-0021228820en_US
dc.identifier.volume14en_US
dc.identifier.issue2en_US
dc.identifier.spage99en_US
dc.identifier.epage121en_US
dc.identifier.isiWOS:A1984TB60000002-
dc.identifier.scopusauthoridHoruk, R=7007108105en_US
dc.identifier.scopusauthoridBeckner, S=7004064238en_US
dc.identifier.scopusauthoridLin, M=7404816359en_US

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