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Article: Multiple expressions of the activity of guanine nucleotide regulatory protein in human thyroid adenylate cyclase

TitleMultiple expressions of the activity of guanine nucleotide regulatory protein in human thyroid adenylate cyclase
Authors
Issue Date1982
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yabbi
Citation
Archives Of Biochemistry And Biophysics, 1982, v. 218 n. 1, p. 8-13 How to Cite?
AbstractAdenylate cyclase (ATP pyrophosphate-lyase, EC 4.6.1.1) in plasma membranes from human thyroid was highly responsive to thyrotropin. Pretreatment of thyroid plasma membranes with 5'-guanylylimidodiphosphate (Gpp(NH)p) in the presence of Mg 2+ led to a temperature-dependent activation, which was seen neither in the absence of Mg 2+ nor at 4°C. By contrast, thyrotropin bound to its receptors regardless of the temperature and produced its maximal effect after 2 min of preincubation in the absence or presence of Mg 2+. Furthermore, activation was seen after treatment with thyrotropin and Gpp(NH)p even carried out in the absence of Mg 2+ or at 4°C. However, the full activation by Gpp(NH)p required Mg 2+, hormone, and elevated temperature. These observations suggest that there appears to be two types of nucleotide interaction responsible for the Gpp(NH)p activation in human thyroid membrane; one type seen in the absence of hormone may represent the system uncoupled from hormone receptor, while the fully coupled hormone-sensitive adenylate cyclase accounts for the second type of interaction which requires the presence of hormone.
Persistent Identifierhttp://hdl.handle.net/10722/167454
ISSN
2015 Impact Factor: 2.807
2015 SCImago Journal Rankings: 1.478
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorAmr, Sen_US
dc.contributor.authorCarayon, Pen_US
dc.contributor.authorLin, MCen_US
dc.date.accessioned2012-10-08T03:07:13Z-
dc.date.available2012-10-08T03:07:13Z-
dc.date.issued1982en_US
dc.identifier.citationArchives Of Biochemistry And Biophysics, 1982, v. 218 n. 1, p. 8-13en_US
dc.identifier.issn0003-9861en_US
dc.identifier.urihttp://hdl.handle.net/10722/167454-
dc.description.abstractAdenylate cyclase (ATP pyrophosphate-lyase, EC 4.6.1.1) in plasma membranes from human thyroid was highly responsive to thyrotropin. Pretreatment of thyroid plasma membranes with 5'-guanylylimidodiphosphate (Gpp(NH)p) in the presence of Mg 2+ led to a temperature-dependent activation, which was seen neither in the absence of Mg 2+ nor at 4°C. By contrast, thyrotropin bound to its receptors regardless of the temperature and produced its maximal effect after 2 min of preincubation in the absence or presence of Mg 2+. Furthermore, activation was seen after treatment with thyrotropin and Gpp(NH)p even carried out in the absence of Mg 2+ or at 4°C. However, the full activation by Gpp(NH)p required Mg 2+, hormone, and elevated temperature. These observations suggest that there appears to be two types of nucleotide interaction responsible for the Gpp(NH)p activation in human thyroid membrane; one type seen in the absence of hormone may represent the system uncoupled from hormone receptor, while the fully coupled hormone-sensitive adenylate cyclase accounts for the second type of interaction which requires the presence of hormone.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yabbien_US
dc.relation.ispartofArchives of Biochemistry and Biophysicsen_US
dc.subject.meshAdenylate Cyclase - Isolation & Purificationen_US
dc.subject.meshBlood Proteins - Metabolismen_US
dc.subject.meshChemical Phenomenaen_US
dc.subject.meshChemistryen_US
dc.subject.meshEnzyme Activation - Drug Effectsen_US
dc.subject.meshGtp-Binding Proteinsen_US
dc.subject.meshGuanine Nucleotides - Metabolismen_US
dc.subject.meshGuanylyl Imidodiphosphate - Pharmacologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMembrane Proteins - Metabolismen_US
dc.subject.meshReceptors, Cell Surface - Metabolismen_US
dc.subject.meshTemperatureen_US
dc.subject.meshThyroid Gland - Enzymologyen_US
dc.subject.meshThyrotropin - Pharmacologyen_US
dc.titleMultiple expressions of the activity of guanine nucleotide regulatory protein in human thyroid adenylate cyclaseen_US
dc.typeArticleen_US
dc.identifier.emailLin, MC:mcllin@hkucc.hku.hken_US
dc.identifier.authorityLin, MC=rp00746en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0003-9861(82)90314-9-
dc.identifier.pmid6293386-
dc.identifier.scopuseid_2-s2.0-0020355027en_US
dc.identifier.volume218en_US
dc.identifier.issue1en_US
dc.identifier.spage8en_US
dc.identifier.epage13en_US
dc.identifier.isiWOS:A1982PK38300002-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridAmr, S=7006019862en_US
dc.identifier.scopusauthoridCarayon, P=7101779952en_US
dc.identifier.scopusauthoridLin, MC=7404816359en_US

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