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Article: Effects of GTP on binding of [ 3H]glucagon to receptors in rat hepatic plasma membranes
Title | Effects of GTP on binding of [ 3H]glucagon to receptors in rat hepatic plasma membranes |
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Authors | |
Issue Date | 1977 |
Publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ |
Citation | Journal Of Biological Chemistry, 1977, v. 252 n. 8, p. 2790-2792 How to Cite? |
Abstract | In this study, we report the preparation of [ 3H]glucagon and its characteristics of binding to receptors in the rat liver plasma membrane. Binding of the labeled hormone is optimal at pH 7.0. In the absence of GTP, [ 3H]glucagon binding to receptors is slow and the time of equilibration is inversely proportional to the hormone concentration. In the presence of GTP, equilibrium is reached within 30 s regardless of hormone levels, and the kinetics of binding are in accord with the kinetics of activation of adenylate cyclase by native glucagon in the presence of the nucleotide. Equilibrium binding measurements indicate that, in the absence of GTP, the binding isotherm is sigmoidal with an apparent K(d) of 2 nM. The addition of GTP results in a complex binding isotherm with about 90% of the binding sites having a considerably lower apparent dissociation constant (>10 nM) and a small population of sites having high affinity for the hormone. The binding properties of [ 3H]glucagon are compared with those of 125I-glucagon, and the implications of the actions of GTP on glucagon binding are discussed in relation to the overall regulation of adenylate cyclase by hormone and the nucleotide. |
Persistent Identifier | http://hdl.handle.net/10722/167442 |
ISSN | 2020 Impact Factor: 5.157 2023 SCImago Journal Rankings: 1.766 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lin, MC | en_US |
dc.contributor.author | Nicosia, S | en_US |
dc.contributor.author | Lad, PM | en_US |
dc.contributor.author | Rodbell, M | en_US |
dc.date.accessioned | 2012-10-08T03:07:02Z | - |
dc.date.available | 2012-10-08T03:07:02Z | - |
dc.date.issued | 1977 | en_US |
dc.identifier.citation | Journal Of Biological Chemistry, 1977, v. 252 n. 8, p. 2790-2792 | en_US |
dc.identifier.issn | 0021-9258 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/167442 | - |
dc.description.abstract | In this study, we report the preparation of [ 3H]glucagon and its characteristics of binding to receptors in the rat liver plasma membrane. Binding of the labeled hormone is optimal at pH 7.0. In the absence of GTP, [ 3H]glucagon binding to receptors is slow and the time of equilibration is inversely proportional to the hormone concentration. In the presence of GTP, equilibrium is reached within 30 s regardless of hormone levels, and the kinetics of binding are in accord with the kinetics of activation of adenylate cyclase by native glucagon in the presence of the nucleotide. Equilibrium binding measurements indicate that, in the absence of GTP, the binding isotherm is sigmoidal with an apparent K(d) of 2 nM. The addition of GTP results in a complex binding isotherm with about 90% of the binding sites having a considerably lower apparent dissociation constant (>10 nM) and a small population of sites having high affinity for the hormone. The binding properties of [ 3H]glucagon are compared with those of 125I-glucagon, and the implications of the actions of GTP on glucagon binding are discussed in relation to the overall regulation of adenylate cyclase by hormone and the nucleotide. | en_US |
dc.language | eng | en_US |
dc.publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ | en_US |
dc.relation.ispartof | Journal of Biological Chemistry | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Binding, Competitive | en_US |
dc.subject.mesh | Cell Membrane - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Glucagon - Metabolism | en_US |
dc.subject.mesh | Guanosine Triphosphate - Pharmacology | en_US |
dc.subject.mesh | Hydrogen-Ion Concentration | en_US |
dc.subject.mesh | Kinetics | en_US |
dc.subject.mesh | Liver - Metabolism | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Receptors, Cell Surface - Drug Effects - Metabolism | en_US |
dc.title | Effects of GTP on binding of [ 3H]glucagon to receptors in rat hepatic plasma membranes | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lin, MC:mcllin@hkucc.hku.hk | en_US |
dc.identifier.authority | Lin, MC=rp00746 | en_US |
dc.description.nature | link_to_OA_fulltext | en_US |
dc.identifier.pmid | 16000 | - |
dc.identifier.scopus | eid_2-s2.0-0017647745 | en_US |
dc.identifier.volume | 252 | en_US |
dc.identifier.issue | 8 | en_US |
dc.identifier.spage | 2790 | en_US |
dc.identifier.epage | 2792 | en_US |
dc.identifier.isi | WOS:A1977DD63900049 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Lin, MC=7404816359 | en_US |
dc.identifier.scopusauthorid | Nicosia, S=7102299447 | en_US |
dc.identifier.scopusauthorid | Lad, PM=7006446792 | en_US |
dc.identifier.scopusauthorid | Rodbell, M=7006658086 | en_US |
dc.identifier.issnl | 0021-9258 | - |