File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: The hepatic adenylate cyclase system. I. Evidence for transition states and structural requirements for guanine nucleotide activation

TitleThe hepatic adenylate cyclase system. I. Evidence for transition states and structural requirements for guanine nucleotide activation
Authors
Issue Date1975
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 1975, v. 250 n. 11, p. 4239-4245 How to Cite?
AbstractPrevious studies showed that guanine nucleotides, acting at a site termed the nucleotide regulatory site, are required for activation of hepatic adenylate cyclase, and that glucagon facilitates this process. This study shows that only guanine nucleotides containing triphosphate groups at the 5' position of ribose (or 3' deoxyribose) are capable of activating the enzyme. The terminal phosphate is not utilized in the activation process, since 5' guanylylimidodiphosphate [Gpp(NH)p] and 5' guanylyl methylenediphosphonate, analogues of guanosine triphosphate that are not utilized in transferase or hydrolase reactions, stimulate enzyme activity. The nucleotides bind in their free form at the regulatory site; chelation by magnesium ion shifts the apparent concentration dependence for activation by Gpp(NH)p. Guanosine diphosphate inhibits competitively Gpp(NH)p stimulated activity and inhibits basal activity and activities stimulated by glucagon.
Persistent Identifierhttp://hdl.handle.net/10722/167436
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSalomon, Yen_US
dc.contributor.authorLin, MCen_US
dc.contributor.authorLondos, Cen_US
dc.date.accessioned2012-10-08T03:06:58Z-
dc.date.available2012-10-08T03:06:58Z-
dc.date.issued1975en_US
dc.identifier.citationJournal Of Biological Chemistry, 1975, v. 250 n. 11, p. 4239-4245en_US
dc.identifier.issn0021-9258en_US
dc.identifier.urihttp://hdl.handle.net/10722/167436-
dc.description.abstractPrevious studies showed that guanine nucleotides, acting at a site termed the nucleotide regulatory site, are required for activation of hepatic adenylate cyclase, and that glucagon facilitates this process. This study shows that only guanine nucleotides containing triphosphate groups at the 5' position of ribose (or 3' deoxyribose) are capable of activating the enzyme. The terminal phosphate is not utilized in the activation process, since 5' guanylylimidodiphosphate [Gpp(NH)p] and 5' guanylyl methylenediphosphonate, analogues of guanosine triphosphate that are not utilized in transferase or hydrolase reactions, stimulate enzyme activity. The nucleotides bind in their free form at the regulatory site; chelation by magnesium ion shifts the apparent concentration dependence for activation by Gpp(NH)p. Guanosine diphosphate inhibits competitively Gpp(NH)p stimulated activity and inhibits basal activity and activities stimulated by glucagon.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.subject.meshAdenylate Cyclase - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBinding, Competitiveen_US
dc.subject.meshCell Membrane - Drug Effects - Enzymologyen_US
dc.subject.meshEnzyme Activation - Drug Effectsen_US
dc.subject.meshGlucagon - Pharmacologyen_US
dc.subject.meshGuanine Nucleotides - Pharmacologyen_US
dc.subject.meshGuanosine Triphosphate - Pharmacologyen_US
dc.subject.meshKineticsen_US
dc.subject.meshLiver - Drug Effects - Enzymologyen_US
dc.subject.meshMagnesium - Pharmacologyen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshRatsen_US
dc.subject.meshStructure-Activity Relationshipen_US
dc.subject.meshTime Factorsen_US
dc.titleThe hepatic adenylate cyclase system. I. Evidence for transition states and structural requirements for guanine nucleotide activationen_US
dc.typeArticleen_US
dc.identifier.emailLin, MC:mcllin@hkucc.hku.hken_US
dc.identifier.authorityLin, MC=rp00746en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.pmid1126949-
dc.identifier.scopuseid_2-s2.0-0016812088en_US
dc.identifier.volume250en_US
dc.identifier.issue11en_US
dc.identifier.spage4239en_US
dc.identifier.epage4245en_US
dc.identifier.isiWOS:A1975AE57800029-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSalomon, Y=7006956498en_US
dc.identifier.scopusauthoridLin, MC=7404816359en_US
dc.identifier.scopusauthoridLondos, C=7005200523en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats