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- Publisher Website: 10.1073/pnas.71.8.3087
- Scopus: eid_2-s2.0-0016264821
- PMID: 4607368
- WOS: WOS:A1974U269600037
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Article: 5'-Guanylylimidodiphosphate, a potent activator of adenylate cyclase systems in eukaryotic cells
Title | 5'-Guanylylimidodiphosphate, a potent activator of adenylate cyclase systems in eukaryotic cells |
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Authors | |
Issue Date | 1974 |
Publisher | National Academy of Sciences. The Journal's web site is located at http://www.pnas.org |
Citation | Proceedings of the National Academy of Sciences of the United States of America, 1974, v. 71 n. 8, p. 3087-3090 How to Cite? |
Abstract | 5' Guanylylimidodiphosphate (Gpp(NH)p) stimulates adenylate cyclase [ATP pyrophosphate lyase (cyclizing), EC 4.6.1.1] activity in plasma membranes isolated from frog and salmon erythrocytes, from rat adrenal, hepatic, and fat cells, and from bovine thyroid cells. The nucleotide acts cooperatively with the various hormones (glucagon, secretin, ACTH, thyrotropin, and catecholamines) that stimulate these adenylate cyclase systems with resultant activities that equal or exceed those obtained with hormone plus GTP or with fluoride ion. In the absence of hormones, Gpp(NH)p is a considerably more effective activator than GTP, and, under certain conditions of incubation, stimulates rat fat cell adenylate cyclase to levels of activity (about 20 nmoles of 3',5' adenosine monophosphate mg protein per min) far higher than reported hitherto for any adenylate cyclase system examined. The nucleotide activates frog erythrocyte adenylate cyclase when the catecholamine receptor is blocked by the competitive antagonist, propranolol, and activates the enzyme from an adrenal tumor cell line which lacks functional ACTH receptors. In contrast, Gpp(NH)p does not stimulate adenylate cyclase in extracts from Escherichia coli B. Gpp(NH)p appears to be a useful probe for investigating the mechanism of hormone and nucleotide action on adenylate cyclase systems in eukaryotic cells. |
Persistent Identifier | http://hdl.handle.net/10722/167431 |
ISSN | 2023 Impact Factor: 9.4 2023 SCImago Journal Rankings: 3.737 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Londos, C | en_US |
dc.contributor.author | Salomon, Y | en_US |
dc.contributor.author | Lin, MC | en_US |
dc.date.accessioned | 2012-10-08T03:06:54Z | - |
dc.date.available | 2012-10-08T03:06:54Z | - |
dc.date.issued | 1974 | en_US |
dc.identifier.citation | Proceedings of the National Academy of Sciences of the United States of America, 1974, v. 71 n. 8, p. 3087-3090 | en_US |
dc.identifier.issn | 0027-8424 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/167431 | - |
dc.description.abstract | 5' Guanylylimidodiphosphate (Gpp(NH)p) stimulates adenylate cyclase [ATP pyrophosphate lyase (cyclizing), EC 4.6.1.1] activity in plasma membranes isolated from frog and salmon erythrocytes, from rat adrenal, hepatic, and fat cells, and from bovine thyroid cells. The nucleotide acts cooperatively with the various hormones (glucagon, secretin, ACTH, thyrotropin, and catecholamines) that stimulate these adenylate cyclase systems with resultant activities that equal or exceed those obtained with hormone plus GTP or with fluoride ion. In the absence of hormones, Gpp(NH)p is a considerably more effective activator than GTP, and, under certain conditions of incubation, stimulates rat fat cell adenylate cyclase to levels of activity (about 20 nmoles of 3',5' adenosine monophosphate mg protein per min) far higher than reported hitherto for any adenylate cyclase system examined. The nucleotide activates frog erythrocyte adenylate cyclase when the catecholamine receptor is blocked by the competitive antagonist, propranolol, and activates the enzyme from an adrenal tumor cell line which lacks functional ACTH receptors. In contrast, Gpp(NH)p does not stimulate adenylate cyclase in extracts from Escherichia coli B. Gpp(NH)p appears to be a useful probe for investigating the mechanism of hormone and nucleotide action on adenylate cyclase systems in eukaryotic cells. | en_US |
dc.language | eng | en_US |
dc.publisher | National Academy of Sciences. The Journal's web site is located at http://www.pnas.org | en_US |
dc.relation.ispartof | Proceedings of the National Academy of Sciences of the United States of America | en_US |
dc.subject.mesh | Adenylate Cyclase - Metabolism | en_US |
dc.subject.mesh | Adipose Tissue - Enzymology | en_US |
dc.subject.mesh | Adrenal Gland Neoplasms - Enzymology | en_US |
dc.subject.mesh | Adrenal Glands - Enzymology | en_US |
dc.subject.mesh | Allosteric Regulation - Drug Effects | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Anura | en_US |
dc.subject.mesh | Cattle | en_US |
dc.subject.mesh | Cell Line | en_US |
dc.subject.mesh | Cell Membrane - Enzymology | en_US |
dc.subject.mesh | Enzyme Activation - Drug Effects | en_US |
dc.subject.mesh | Epinephrine - Pharmacology | en_US |
dc.subject.mesh | Erythrocytes - Enzymology | en_US |
dc.subject.mesh | Escherichia Coli - Enzymology | en_US |
dc.subject.mesh | Fluorides - Pharmacology | en_US |
dc.subject.mesh | Glucagon - Pharmacology | en_US |
dc.subject.mesh | Guanine Nucleotides - Pharmacology | en_US |
dc.subject.mesh | Guanosine Triphosphate - Pharmacology | en_US |
dc.subject.mesh | Liver - Enzymology | en_US |
dc.subject.mesh | Propranolol - Pharmacology | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Salmon | en_US |
dc.subject.mesh | Stimulation, Chemical | en_US |
dc.subject.mesh | Thyroid Gland - Enzymology | en_US |
dc.title | 5'-Guanylylimidodiphosphate, a potent activator of adenylate cyclase systems in eukaryotic cells | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lin, MC:mcllin@hkucc.hku.hk | en_US |
dc.identifier.authority | Lin, MC=rp00746 | en_US |
dc.description.nature | link_to_OA_fulltext | en_US |
dc.identifier.doi | 10.1073/pnas.71.8.3087 | - |
dc.identifier.pmid | 4607368 | - |
dc.identifier.pmcid | PMC388626 | - |
dc.identifier.scopus | eid_2-s2.0-0016264821 | en_US |
dc.identifier.volume | 71 | en_US |
dc.identifier.issue | 8 | en_US |
dc.identifier.spage | 3087 | en_US |
dc.identifier.epage | 3090 | en_US |
dc.identifier.isi | WOS:A1974U269600037 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Londos, C=7005200523 | en_US |
dc.identifier.scopusauthorid | Salomon, Y=7006956498 | en_US |
dc.identifier.scopusauthorid | Lin, MC=7404816359 | en_US |
dc.identifier.issnl | 0027-8424 | - |