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Article: [16] An automated chromatographic assay for adenylate cyclase and cyclic 3′,5′-AMP phosphodiesterase

Title[16] An automated chromatographic assay for adenylate cyclase and cyclic 3′,5′-AMP phosphodiesterase
Authors
Issue Date1974
PublisherAcademic Press. The Journal's web site is located at http://www.sciencedirect.com/science/bookseries/00766879
Citation
Methods In Enzymology, 1974, v. 38 C, p. 125-135 How to Cite?
AbstractThe establishment of cyclic 3', 5'-AMP (cAMP) as a second messenger in numerous hormonal regulations has increased the need for efficient assays for adenylate cyclase and for cyclic 3', 5'-AMP phosphodiesterase. Adenylate cyclase preparations normally contain various enzymatic activities, such as ATPase, phosphodiesterase, and other phosphatases; therefore, numerous by-products other than cAMP are formed during the assay. The success of the procedure depends on the complete separation of cAMP, which often amounts to as low as 0.01 to 0.1% of the ATP substrate, from other adenine nucleotides. Adenylate cyclase was first measured by an assay which employed coupled enzyme systems. The automated procedure described in this chapter was developed to provide a convenient means for assaying adenylate cyclase activity in “solubilized” preparation from bovine brain; the same technique has proved adaptable for the assay of phosphodiesterase activity.
Persistent Identifierhttp://hdl.handle.net/10722/167428
ISSN
2014 Impact Factor: 2.088
2015 SCImago Journal Rankings: 1.501

 

DC FieldValueLanguage
dc.contributor.authorLin, MCen_US
dc.date.accessioned2012-10-08T03:06:50Z-
dc.date.available2012-10-08T03:06:50Z-
dc.date.issued1974en_US
dc.identifier.citationMethods In Enzymology, 1974, v. 38 C, p. 125-135en_US
dc.identifier.issn0076-6879en_US
dc.identifier.urihttp://hdl.handle.net/10722/167428-
dc.description.abstractThe establishment of cyclic 3', 5'-AMP (cAMP) as a second messenger in numerous hormonal regulations has increased the need for efficient assays for adenylate cyclase and for cyclic 3', 5'-AMP phosphodiesterase. Adenylate cyclase preparations normally contain various enzymatic activities, such as ATPase, phosphodiesterase, and other phosphatases; therefore, numerous by-products other than cAMP are formed during the assay. The success of the procedure depends on the complete separation of cAMP, which often amounts to as low as 0.01 to 0.1% of the ATP substrate, from other adenine nucleotides. Adenylate cyclase was first measured by an assay which employed coupled enzyme systems. The automated procedure described in this chapter was developed to provide a convenient means for assaying adenylate cyclase activity in “solubilized” preparation from bovine brain; the same technique has proved adaptable for the assay of phosphodiesterase activity.-
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.sciencedirect.com/science/bookseries/00766879en_US
dc.relation.ispartofMethods in Enzymologyen_US
dc.title[16] An automated chromatographic assay for adenylate cyclase and cyclic 3′,5′-AMP phosphodiesteraseen_US
dc.typeArticleen_US
dc.identifier.emailLin, MC:mcllin@hkucc.hku.hken_US
dc.identifier.authorityLin, MC=rp00746en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0076-6879(74)38019-6en_US
dc.identifier.scopuseid_2-s2.0-0016182372en_US
dc.identifier.volume38en_US
dc.identifier.issueCen_US
dc.identifier.spage125en_US
dc.identifier.epage135en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLin, MC=7404816359en_US

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