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Article: Expression of placental leptin and leptin receptors in preeclampsia

TitleExpression of placental leptin and leptin receptors in preeclampsia
Authors
Issue Date2004
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.intjgynpathology.com
Citation
International Journal Of Gynecological Pathology, 2004, v. 23 n. 4, p. 378-385 How to Cite?
AbstractThis study investigated the expression profile of placental leptin and leptin receptor isoforms in preeclampsia, using placental tissue from normal pregnancies that were matched in gestational age and birth weight as controls. A total of 29 cases of preeclampsia were studied by immunohistochemistry, including 16 severe and 13 mild preeclampsia cases. Reverse transcriptase- polymerase chain reaction (RT-PCR) was further performed using RNA extracted from frozen tissue (10 severe preeclampsia, 10 mild preeclampsia, and 20 normal third trimester placentas). In all tissue sections, immunostaining signal was shown in the cytoplasmic compartment of the trophoblastic cells. Both the severe and mild preeclampsia groups showed significantly higher immunostaining for leptin compared with normal controls (p < 0.05), but there was no significant difference between the severe and mild preeclampsia groups (p > 0.05). There was no significant difference in immunostaining for leptin receptor between both severe and mild preeclampsia compared with controls (p > 0.05). RT-PCR showed significantly higher levels of mRNA transcripts of leptin in severe preeclampsia (p < 0.05), but not mild preeclampsia (p > 0.05), compared with normal controls. No significant difference in expression of all the receptor isoforms was demonstrated between both severe and mild preeclampsia groups compared with controls (p > 0.05). In conclusion, we confirmed an up-regulated expression of leptin in placental tissue in preeclampsia. However, there was no difference in the expression of all leptin receptor isoforms in placental tissue between preeclamptic and normal pregnancies. The leptin signal probably does not play a major primary role in the pathogenesis of preeclampsia.
Persistent Identifierhttp://hdl.handle.net/10722/167090
ISSN
2015 Impact Factor: 1.437
2015 SCImago Journal Rankings: 0.723
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, RHWen_US
dc.contributor.authorPoon, SCSen_US
dc.contributor.authorYu, MYen_US
dc.contributor.authorWong, YFen_US
dc.date.accessioned2012-09-28T04:03:38Z-
dc.date.available2012-09-28T04:03:38Z-
dc.date.issued2004en_US
dc.identifier.citationInternational Journal Of Gynecological Pathology, 2004, v. 23 n. 4, p. 378-385en_US
dc.identifier.issn0277-1691en_US
dc.identifier.urihttp://hdl.handle.net/10722/167090-
dc.description.abstractThis study investigated the expression profile of placental leptin and leptin receptor isoforms in preeclampsia, using placental tissue from normal pregnancies that were matched in gestational age and birth weight as controls. A total of 29 cases of preeclampsia were studied by immunohistochemistry, including 16 severe and 13 mild preeclampsia cases. Reverse transcriptase- polymerase chain reaction (RT-PCR) was further performed using RNA extracted from frozen tissue (10 severe preeclampsia, 10 mild preeclampsia, and 20 normal third trimester placentas). In all tissue sections, immunostaining signal was shown in the cytoplasmic compartment of the trophoblastic cells. Both the severe and mild preeclampsia groups showed significantly higher immunostaining for leptin compared with normal controls (p < 0.05), but there was no significant difference between the severe and mild preeclampsia groups (p > 0.05). There was no significant difference in immunostaining for leptin receptor between both severe and mild preeclampsia compared with controls (p > 0.05). RT-PCR showed significantly higher levels of mRNA transcripts of leptin in severe preeclampsia (p < 0.05), but not mild preeclampsia (p > 0.05), compared with normal controls. No significant difference in expression of all the receptor isoforms was demonstrated between both severe and mild preeclampsia groups compared with controls (p > 0.05). In conclusion, we confirmed an up-regulated expression of leptin in placental tissue in preeclampsia. However, there was no difference in the expression of all leptin receptor isoforms in placental tissue between preeclamptic and normal pregnancies. The leptin signal probably does not play a major primary role in the pathogenesis of preeclampsia.en_US
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.intjgynpathology.comen_US
dc.relation.ispartofInternational Journal of Gynecological Pathologyen_US
dc.subject.meshDna Primersen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshLeptin - Biosynthesisen_US
dc.subject.meshPlacenta - Metabolismen_US
dc.subject.meshPre-Eclampsia - Metabolismen_US
dc.subject.meshPregnancyen_US
dc.subject.meshProtein Isoforms - Biosynthesisen_US
dc.subject.meshReceptors, Cell Surface - Biosynthesisen_US
dc.subject.meshReceptors, Leptinen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.titleExpression of placental leptin and leptin receptors in preeclampsiaen_US
dc.typeArticleen_US
dc.identifier.emailLi, RHW: raymondli@hku.hken_US
dc.identifier.authorityLi, RHW=rp01649en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1097/01.pgp.0000139647.40620.c8en_US
dc.identifier.pmid15381908-
dc.identifier.scopuseid_2-s2.0-4644351726en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-4644351726&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume23en_US
dc.identifier.issue4en_US
dc.identifier.spage378en_US
dc.identifier.epage385en_US
dc.identifier.isiWOS:000224103700010-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLi, RHW=7404724295en_US
dc.identifier.scopusauthoridPoon, SCS=7102884263en_US
dc.identifier.scopusauthoridYu, MY=35076866400en_US
dc.identifier.scopusauthoridWong, YF=7403041448en_US

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