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Article: Two-dimension antigenic dendrogram and phylogenetic tree of avian influenza virus H5N1

TitleTwo-dimension antigenic dendrogram and phylogenetic tree of avian influenza virus H5N1
Authors
KeywordsAntigenic cluster
Antigenic dendrogram
Avian influenza virus H5N1
Hierarchical clustering
Monoclonal antibody
Phylogenetic tree
Issue Date2012
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=0928-8244&site=1
Citation
FEMS Immunology and Medical Microbiology, 2012, v. 64 n. 2, p. 205-211 How to Cite?
AbstractThe hemagglutination-inhibition (HI) titers of a panel of 25 mouse monoclonal antibodies (MAbs) against 44 isolates of highly pathogenic avian influenza virus H5N1 were determined. A two-dimensional antigenic dendrogram was constructed by hierarchical clustering of HI titers. Viruses with similar reactivity patterns were clustered horizontally, whereas MAbs were clustered vertically. In this 2-D dendrogram, with 40% similarity as a cut-off, four virus clusters and four MAbs clusters were identified. A phylogenetic tree based on the deduced amino acid sequence of the hemagglutinin gene of tested viruses was constructed and its topology was compared to the antigenic dendrogram. Interestingly, viruses with high genetic homology in the phylogenetic tree also had high similarity in their reactivity patterns, as indicated by their relatedness in the tree and close clustering in the dendrogram, respectively. However, the reverse and the converse were also true. Of the five pairs of viruses in the dendrogram with bootstrap values higher than 75, four pairs were in concordance with their genetic relatedness. However, one pair contained viruses belong to two distinct genetic clades. These results were discussed in the context of antigenic variation, genetic polymorphism, and the potential application of MAbs in antigenic analysis.
Persistent Identifierhttp://hdl.handle.net/10722/166782
ISSN
2014 Impact Factor: 3.078
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLai, ACKen_US
dc.contributor.authorWu, WLen_US
dc.contributor.authorLau, SYen_US
dc.contributor.authorGuan, Yen_US
dc.contributor.authorChen, Hen_US
dc.date.accessioned2012-09-20T08:48:05Z-
dc.date.available2012-09-20T08:48:05Z-
dc.date.issued2012en_US
dc.identifier.citationFEMS Immunology and Medical Microbiology, 2012, v. 64 n. 2, p. 205-211en_US
dc.identifier.issn0928-8244-
dc.identifier.urihttp://hdl.handle.net/10722/166782-
dc.description.abstractThe hemagglutination-inhibition (HI) titers of a panel of 25 mouse monoclonal antibodies (MAbs) against 44 isolates of highly pathogenic avian influenza virus H5N1 were determined. A two-dimensional antigenic dendrogram was constructed by hierarchical clustering of HI titers. Viruses with similar reactivity patterns were clustered horizontally, whereas MAbs were clustered vertically. In this 2-D dendrogram, with 40% similarity as a cut-off, four virus clusters and four MAbs clusters were identified. A phylogenetic tree based on the deduced amino acid sequence of the hemagglutinin gene of tested viruses was constructed and its topology was compared to the antigenic dendrogram. Interestingly, viruses with high genetic homology in the phylogenetic tree also had high similarity in their reactivity patterns, as indicated by their relatedness in the tree and close clustering in the dendrogram, respectively. However, the reverse and the converse were also true. Of the five pairs of viruses in the dendrogram with bootstrap values higher than 75, four pairs were in concordance with their genetic relatedness. However, one pair contained viruses belong to two distinct genetic clades. These results were discussed in the context of antigenic variation, genetic polymorphism, and the potential application of MAbs in antigenic analysis.-
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=0928-8244&site=1-
dc.relation.ispartofFEMS Immunology and Medical Microbiologyen_US
dc.rightsThe definitive version is available at www.blackwell-synergy.com-
dc.subjectAntigenic cluster-
dc.subjectAntigenic dendrogram-
dc.subjectAvian influenza virus H5N1-
dc.subjectHierarchical clustering-
dc.subjectMonoclonal antibody-
dc.subjectPhylogenetic tree-
dc.subject.meshAntigens, Viral - chemistry - immunology-
dc.subject.meshHemagglutination Inhibition Tests-
dc.subject.meshHemagglutinins, Viral - chemistry - genetics - immunology-
dc.subject.meshInfluenza A Virus, H5N1 Subtype - genetics - immunology-
dc.subject.meshInfluenza in Birds - immunology - virology-
dc.titleTwo-dimension antigenic dendrogram and phylogenetic tree of avian influenza virus H5N1en_US
dc.typeArticleen_US
dc.identifier.emailLai, ACK: lai_alexander@yahoo.comen_US
dc.identifier.emailWu, WL: hazelwu@hkucc.hku.hken_US
dc.identifier.emailLau, SY: sylau926@hkucc.hku.hken_US
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_US
dc.identifier.emailChen, H: hlchen@hku.hk-
dc.identifier.authorityGuan, Y=rp00397en_US
dc.identifier.authorityChen, H=rp00383en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1111/j.1574-695X.2011.00884.x-
dc.identifier.pmid22066850-
dc.identifier.pmcidPMC3288707-
dc.identifier.scopuseid_2-s2.0-84856951428-
dc.identifier.hkuros208504en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84856951428&selection=ref&src=s&origin=recordpage-
dc.identifier.volume64en_US
dc.identifier.issue2-
dc.identifier.spage205en_US
dc.identifier.epage211en_US
dc.identifier.isiWOS:000300050800007-
dc.publisher.placeUnited Kingdom-
dc.identifier.scopusauthoridLai, AC=36769811700-
dc.identifier.scopusauthoridWu, WL=16835478800-
dc.identifier.scopusauthoridLau, SY=35083705100-
dc.identifier.scopusauthoridGuan, Y=7202924055-
dc.identifier.scopusauthoridChen, H=26643315400-

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