File Download
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: Two-dimension antigenic dendrogram and phylogenetic tree of avian influenza virus H5N1
  • Basic View
  • Metadata View
  • XML View
TitleTwo-dimension antigenic dendrogram and phylogenetic tree of avian influenza virus H5N1
 
AuthorsLai, ACK
Wu, WL1
Lau, SY1
Guan, Y1
Chen, H1
 
KeywordsAntigenic cluster
Antigenic dendrogram
Avian influenza virus H5N1
Hierarchical clustering
Monoclonal antibody
Phylogenetic tree
 
Issue Date2012
 
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=0928-8244&site=1
 
CitationFEMS Immunology and Medical Microbiology, 2012, v. 64 n. 2, p. 205-211 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1574-695X.2011.00884.x
 
AbstractThe hemagglutination-inhibition (HI) titers of a panel of 25 mouse monoclonal antibodies (MAbs) against 44 isolates of highly pathogenic avian influenza virus H5N1 were determined. A two-dimensional antigenic dendrogram was constructed by hierarchical clustering of HI titers. Viruses with similar reactivity patterns were clustered horizontally, whereas MAbs were clustered vertically. In this 2-D dendrogram, with 40% similarity as a cut-off, four virus clusters and four MAbs clusters were identified. A phylogenetic tree based on the deduced amino acid sequence of the hemagglutinin gene of tested viruses was constructed and its topology was compared to the antigenic dendrogram. Interestingly, viruses with high genetic homology in the phylogenetic tree also had high similarity in their reactivity patterns, as indicated by their relatedness in the tree and close clustering in the dendrogram, respectively. However, the reverse and the converse were also true. Of the five pairs of viruses in the dendrogram with bootstrap values higher than 75, four pairs were in concordance with their genetic relatedness. However, one pair contained viruses belong to two distinct genetic clades. These results were discussed in the context of antigenic variation, genetic polymorphism, and the potential application of MAbs in antigenic analysis.
 
ISSN0928-8244
2013 Impact Factor: 2.554
 
DOIhttp://dx.doi.org/10.1111/j.1574-695X.2011.00884.x
 
PubMed Central IDPMC3288707
 
ISI Accession Number IDWOS:000300050800007
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLai, ACK
 
dc.contributor.authorWu, WL
 
dc.contributor.authorLau, SY
 
dc.contributor.authorGuan, Y
 
dc.contributor.authorChen, H
 
dc.date.accessioned2012-09-20T08:48:05Z
 
dc.date.available2012-09-20T08:48:05Z
 
dc.date.issued2012
 
dc.description.abstractThe hemagglutination-inhibition (HI) titers of a panel of 25 mouse monoclonal antibodies (MAbs) against 44 isolates of highly pathogenic avian influenza virus H5N1 were determined. A two-dimensional antigenic dendrogram was constructed by hierarchical clustering of HI titers. Viruses with similar reactivity patterns were clustered horizontally, whereas MAbs were clustered vertically. In this 2-D dendrogram, with 40% similarity as a cut-off, four virus clusters and four MAbs clusters were identified. A phylogenetic tree based on the deduced amino acid sequence of the hemagglutinin gene of tested viruses was constructed and its topology was compared to the antigenic dendrogram. Interestingly, viruses with high genetic homology in the phylogenetic tree also had high similarity in their reactivity patterns, as indicated by their relatedness in the tree and close clustering in the dendrogram, respectively. However, the reverse and the converse were also true. Of the five pairs of viruses in the dendrogram with bootstrap values higher than 75, four pairs were in concordance with their genetic relatedness. However, one pair contained viruses belong to two distinct genetic clades. These results were discussed in the context of antigenic variation, genetic polymorphism, and the potential application of MAbs in antigenic analysis.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationFEMS Immunology and Medical Microbiology, 2012, v. 64 n. 2, p. 205-211 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1574-695X.2011.00884.x
 
dc.identifier.doihttp://dx.doi.org/10.1111/j.1574-695X.2011.00884.x
 
dc.identifier.epage211
 
dc.identifier.hkuros208504
 
dc.identifier.isiWOS:000300050800007
 
dc.identifier.issn0928-8244
2013 Impact Factor: 2.554
 
dc.identifier.issue2
 
dc.identifier.pmcidPMC3288707
 
dc.identifier.pmid22066850
 
dc.identifier.scopuseid_2-s2.0-84856951428
 
dc.identifier.spage205
 
dc.identifier.urihttp://hdl.handle.net/10722/166782
 
dc.identifier.volume64
 
dc.languageeng
 
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=0928-8244&site=1
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofFEMS Immunology and Medical Microbiology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsThe definitive version is available at www.blackwell-synergy.com
 
dc.subject.meshAntigens, Viral - chemistry - immunology
 
dc.subject.meshHemagglutination Inhibition Tests
 
dc.subject.meshHemagglutinins, Viral - chemistry - genetics - immunology
 
dc.subject.meshInfluenza A Virus, H5N1 Subtype - genetics - immunology
 
dc.subject.meshInfluenza in Birds - immunology - virology
 
dc.subjectAntigenic cluster
 
dc.subjectAntigenic dendrogram
 
dc.subjectAvian influenza virus H5N1
 
dc.subjectHierarchical clustering
 
dc.subjectMonoclonal antibody
 
dc.subjectPhylogenetic tree
 
dc.titleTwo-dimension antigenic dendrogram and phylogenetic tree of avian influenza virus H5N1
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Lai, ACK</contributor.author>
<contributor.author>Wu, WL</contributor.author>
<contributor.author>Lau, SY</contributor.author>
<contributor.author>Guan, Y</contributor.author>
<contributor.author>Chen, H</contributor.author>
<date.accessioned>2012-09-20T08:48:05Z</date.accessioned>
<date.available>2012-09-20T08:48:05Z</date.available>
<date.issued>2012</date.issued>
<identifier.citation>FEMS Immunology and Medical Microbiology, 2012, v. 64 n. 2, p. 205-211</identifier.citation>
<identifier.issn>0928-8244</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/166782</identifier.uri>
<description.abstract>The hemagglutination-inhibition (HI) titers of a panel of 25 mouse monoclonal antibodies (MAbs) against 44 isolates of highly pathogenic avian influenza virus H5N1 were determined. A two-dimensional antigenic dendrogram was constructed by hierarchical clustering of HI titers. Viruses with similar reactivity patterns were clustered horizontally, whereas MAbs were clustered vertically. In this 2-D dendrogram, with 40% similarity as a cut-off, four virus clusters and four MAbs clusters were identified. A phylogenetic tree based on the deduced amino acid sequence of the hemagglutinin gene of tested viruses was constructed and its topology was compared to the antigenic dendrogram. Interestingly, viruses with high genetic homology in the phylogenetic tree also had high similarity in their reactivity patterns, as indicated by their relatedness in the tree and close clustering in the dendrogram, respectively. However, the reverse and the converse were also true. Of the five pairs of viruses in the dendrogram with bootstrap values higher than 75, four pairs were in concordance with their genetic relatedness. However, one pair contained viruses belong to two distinct genetic clades. These results were discussed in the context of antigenic variation, genetic polymorphism, and the potential application of MAbs in antigenic analysis.</description.abstract>
<language>eng</language>
<publisher>Blackwell Publishing Ltd. The Journal&apos;s web site is located at http://www.blackwellpublishing.com/journal.asp?ref=0928-8244&amp;site=1</publisher>
<relation.ispartof>FEMS Immunology and Medical Microbiology</relation.ispartof>
<rights>The definitive version is available at www.blackwell-synergy.com</rights>
<subject>Antigenic cluster</subject>
<subject>Antigenic dendrogram</subject>
<subject>Avian influenza virus H5N1</subject>
<subject>Hierarchical clustering</subject>
<subject>Monoclonal antibody</subject>
<subject>Phylogenetic tree</subject>
<subject.mesh>Antigens, Viral - chemistry - immunology</subject.mesh>
<subject.mesh>Hemagglutination Inhibition Tests</subject.mesh>
<subject.mesh>Hemagglutinins, Viral - chemistry - genetics - immunology</subject.mesh>
<subject.mesh>Influenza A Virus, H5N1 Subtype - genetics - immunology</subject.mesh>
<subject.mesh>Influenza in Birds - immunology - virology</subject.mesh>
<title>Two-dimension antigenic dendrogram and phylogenetic tree of avian influenza virus H5N1</title>
<type>Article</type>
<description.nature>link_to_OA_fulltext</description.nature>
<identifier.doi>10.1111/j.1574-695X.2011.00884.x</identifier.doi>
<identifier.pmid>22066850</identifier.pmid>
<identifier.pmcid>PMC3288707</identifier.pmcid>
<identifier.scopus>eid_2-s2.0-84856951428</identifier.scopus>
<identifier.hkuros>208504</identifier.hkuros>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-84856951428&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>64</identifier.volume>
<identifier.issue>2</identifier.issue>
<identifier.spage>205</identifier.spage>
<identifier.epage>211</identifier.epage>
<identifier.isi>WOS:000300050800007</identifier.isi>
<publisher.place>United Kingdom</publisher.place>
<bitstream.url>http://hub.hku.hk/bitstream/10722/166782/1/re01.htm</bitstream.url>
</item>
Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine