Article: Two-dimension antigenic dendrogram and phylogenetic tree of avian influenza virus H5N1

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Publisher Website: 10.1111/j.1574-695X.2011.00884.x
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Title	Two-dimension antigenic dendrogram and phylogenetic tree of avian influenza virus H5N1
Authors	Lai, ACK Wu, WL1 Lau, SY1 Guan, Y1 Chen, H1
Keywords	Antigenic cluster Antigenic dendrogram Avian influenza virus H5N1 Hierarchical clustering Monoclonal antibody Phylogenetic tree
Issue Date	2012
Publisher	Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=0928-8244&site=1
Citation	FEMS Immunology and Medical Microbiology, 2012, v. 64 n. 2, p. 205-211 [How to Cite?] DOI: http://dx.doi.org/10.1111/j.1574-695X.2011.00884.x
Abstract	The hemagglutination-inhibition (HI) titers of a panel of 25 mouse monoclonal antibodies (MAbs) against 44 isolates of highly pathogenic avian influenza virus H5N1 were determined. A two-dimensional antigenic dendrogram was constructed by hierarchical clustering of HI titers. Viruses with similar reactivity patterns were clustered horizontally, whereas MAbs were clustered vertically. In this 2-D dendrogram, with 40% similarity as a cut-off, four virus clusters and four MAbs clusters were identified. A phylogenetic tree based on the deduced amino acid sequence of the hemagglutinin gene of tested viruses was constructed and its topology was compared to the antigenic dendrogram. Interestingly, viruses with high genetic homology in the phylogenetic tree also had high similarity in their reactivity patterns, as indicated by their relatedness in the tree and close clustering in the dendrogram, respectively. However, the reverse and the converse were also true. Of the five pairs of viruses in the dendrogram with bootstrap values higher than 75, four pairs were in concordance with their genetic relatedness. However, one pair contained viruses belong to two distinct genetic clades. These results were discussed in the context of antigenic variation, genetic polymorphism, and the potential application of MAbs in antigenic analysis.
ISSN	0928-8244 2011 Impact Factor: 2.441 2011 SCImago Journal Rankings: 0.198
DOI	http://dx.doi.org/10.1111/j.1574-695X.2011.00884.x
ISI Accession Number ID	WOS:000300050800007
PubMed Central ID	PMC3288707
References	References in Scopus

DC Field	Value
dc.contributor.author	Lai, ACK
dc.contributor.author	Wu, WL
dc.contributor.author	Lau, SY
dc.contributor.author	Guan, Y
dc.contributor.author	Chen, H
dc.date.accessioned	2012-09-20T08:48:05Z
dc.date.available	2012-09-20T08:48:05Z
dc.date.issued	2012
dc.description.abstract	The hemagglutination-inhibition (HI) titers of a panel of 25 mouse monoclonal antibodies (MAbs) against 44 isolates of highly pathogenic avian influenza virus H5N1 were determined. A two-dimensional antigenic dendrogram was constructed by hierarchical clustering of HI titers. Viruses with similar reactivity patterns were clustered horizontally, whereas MAbs were clustered vertically. In this 2-D dendrogram, with 40% similarity as a cut-off, four virus clusters and four MAbs clusters were identified. A phylogenetic tree based on the deduced amino acid sequence of the hemagglutinin gene of tested viruses was constructed and its topology was compared to the antigenic dendrogram. Interestingly, viruses with high genetic homology in the phylogenetic tree also had high similarity in their reactivity patterns, as indicated by their relatedness in the tree and close clustering in the dendrogram, respectively. However, the reverse and the converse were also true. Of the five pairs of viruses in the dendrogram with bootstrap values higher than 75, four pairs were in concordance with their genetic relatedness. However, one pair contained viruses belong to two distinct genetic clades. These results were discussed in the context of antigenic variation, genetic polymorphism, and the potential application of MAbs in antigenic analysis.
dc.description.nature	link_to_OA_fulltext
dc.identifier.citation	FEMS Immunology and Medical Microbiology, 2012, v. 64 n. 2, p. 205-211 [How to Cite?] DOI: http://dx.doi.org/10.1111/j.1574-695X.2011.00884.x
dc.identifier.doi	http://dx.doi.org/10.1111/j.1574-695X.2011.00884.x
dc.identifier.epage	211
dc.identifier.hkuros	208504
dc.identifier.isi	WOS:000300050800007
dc.identifier.issn	0928-8244 2011 Impact Factor: 2.441 2011 SCImago Journal Rankings: 0.198
dc.identifier.issue	2
dc.identifier.pmcid	PMC3288707
dc.identifier.pmid	22066850
dc.identifier.scopus	eid_2-s2.0-84856951428
dc.identifier.spage	205
dc.identifier.uri	http://hdl.handle.net/10722/166782
dc.identifier.volume	64
dc.language	eng
dc.publisher	Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=0928-8244&site=1
dc.publisher.place	United Kingdom
dc.relation.ispartof	FEMS Immunology and Medical Microbiology
dc.relation.references	References in Scopus
dc.rights	The definitive version is available at www.blackwell-synergy.com
dc.subject.mesh	Antigens, Viral - chemistry - immunology
dc.subject.mesh	Hemagglutination Inhibition Tests
dc.subject.mesh	Hemagglutinins, Viral - chemistry - genetics - immunology
dc.subject.mesh	Influenza A Virus, H5N1 Subtype - genetics - immunology
dc.subject.mesh	Influenza in Birds - immunology - virology
dc.subject	Antigenic cluster
dc.subject	Antigenic dendrogram
dc.subject	Avian influenza virus H5N1
dc.subject	Hierarchical clustering
dc.subject	Monoclonal antibody
dc.subject	Phylogenetic tree
dc.title	Two-dimension antigenic dendrogram and phylogenetic tree of avian influenza virus H5N1
dc.type	Article

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Author Affiliations

The University of Hong Kong Li Ka Shing Faculty of Medicine